+Open data
-Basic information
Entry | Database: PDB / ID: 1msc | ||||||
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Title | CRYSTAL STRUCTURE OF MS2 COAT PROTEIN DIMER | ||||||
Components | BACTERIOPHAGE MS2 COAT PROTEIN | ||||||
Keywords | VIRAL PROTEIN / TRANSLATION REPRESSOR | ||||||
Function / homology | Function and homology information negative regulation of viral translation / T=3 icosahedral viral capsid / regulation of translation / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | Enterobacterio phage MS2 (virus) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Ni, C.-Z. / Kodandapani, R. / Ely, K.R. | ||||||
Citation | Journal: Structure / Year: 1995 Title: Crystal structure of the MS2 coat protein dimer: implications for RNA binding and virus assembly. Authors: Ni, C.Z. / Syed, R. / Kodandapani, R. / Wickersham, J. / Peabody, D.S. / Ely, K.R. #1: Journal: Protein Sci. / Year: 1995 Title: Crystallization of the MS2 Translational Repressor Alone and Complexed to Bromouridine Authors: Ni, C.-Z. / Hettinga, B.S. / Wickersham, J. / Mitchell, R.S. / Williamson, M.M. / Celikel, R. / Prange, T. / Fourme, R. / Krapcho, K.J. / Thulin, C. / Talbot, P. / Gesteland, R.F. / Ely, K.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1msc.cif.gz | 40 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1msc.ent.gz | 27.9 KB | Display | PDB format |
PDBx/mmJSON format | 1msc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1msc_validation.pdf.gz | 366 KB | Display | wwPDB validaton report |
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Full document | 1msc_full_validation.pdf.gz | 387.7 KB | Display | |
Data in XML | 1msc_validation.xml.gz | 7.5 KB | Display | |
Data in CIF | 1msc_validation.cif.gz | 10.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ms/1msc ftp://data.pdbj.org/pub/pdb/validation_reports/ms/1msc | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13709.448 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacterio phage MS2 (virus) / Genus: Levivirus / Species: Enterobacteria phage MS2 / Gene: MS2 COAT GENE / Gene (production host): MS2 COAT GENE / Production host: Escherichia coli (E. coli) / References: UniProt: P03612 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.02 % | ||||||||||||||||||||
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Crystal grow | Details: COMPND MOLECULE: MS2 UNASSEMBLED COAT PROTEIN DIMER. THIS BACTERIOPHAGE COAT PROTEIN WAS CRYSTALLIZED AS AN UNASSEMBLED DIMER; IT DID NOT FORM VIRAL CAPSIDS. | ||||||||||||||||||||
Crystal grow | *PLUS pH: 6.2 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Detector | Date: Dec 7, 1993 |
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Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.03→55 Å / Num. obs: 7447 / % possible obs: 90 % / Redundancy: 3.2 % / Rmerge(I) obs: 0.031 |
Reflection | *PLUS Num. measured all: 23935 / Rmerge(I) obs: 0.031 |
Reflection shell | *PLUS Highest resolution: 2.03 Å / Lowest resolution: 2.19 Å / % possible obs: 65 % / Num. unique obs: 1033 / Rmerge(I) obs: 0.107 / Mean I/σ(I) obs: 5.7 |
-Processing
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Refinement | Resolution: 2→8 Å / σ(F): 3 /
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Displacement parameters | Biso mean: 34.47 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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