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Yorodumi- PDB-1k19: NMR Solution Structure of the Chemosensory Protein CSP2 from Moth... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1k19 | ||||||
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Title | NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae | ||||||
Components | Chemosensory Protein CSP2 | ||||||
Keywords | LIPID TRANSPORT / Chemosensory / Pheromone | ||||||
Function / homology | Antennal chemosensory protein a6 / Insect odorant-binding protein A10/Ejaculatory bulb-specific protein 3 / Insect odorant-binding protein A10/Ejaculatory bulb-specific protein 3 / Insect odorant-binding protein A10/Ejaculatory bulb-specific protein 3 superfamily / Insect pheromone-binding family, A10/OS-D / Orthogonal Bundle / Mainly Alpha / Chemosensory protein Function and homology information | ||||||
Biological species | Mamestra brassicae (cabbage moth) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Mosbah, A. / Campanacci, V. / Lartigue, A. / Tegoni, M. / Cambillau, C. / Darbon, H. | ||||||
Citation | Journal: BIOCHEM.J. / Year: 2003 Title: Solution structure of a chemosensory protein from the moth Mamestra brassicae Authors: Mosbah, A. / Campanacci, V. / Lartigue, A. / Tegoni, M. / Cambillau, C. / Darbon, H. #1: Journal: Eur.J.Biochem. / Year: 2001 Title: Chemosensory Protein from the moth Mamestra brassicae. Expression and secondary structure from 1H and 15N NMR Authors: Campanacci, V. / Mosbah, A. / Bornet, O. / Wechselberger, R. / Jacquin-Joly, E. / Cambillau, C. / Darbon, H. / Tegoni, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1k19.cif.gz | 706.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1k19.ent.gz | 611.3 KB | Display | PDB format |
PDBx/mmJSON format | 1k19.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1k19_validation.pdf.gz | 353.8 KB | Display | wwPDB validaton report |
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Full document | 1k19_full_validation.pdf.gz | 574.7 KB | Display | |
Data in XML | 1k19_validation.xml.gz | 69.1 KB | Display | |
Data in CIF | 1k19_validation.cif.gz | 92.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k1/1k19 ftp://data.pdbj.org/pub/pdb/validation_reports/k1/1k19 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13094.840 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mamestra brassicae (cabbage moth) / Production host: Escherichia coli (E. coli) / References: UniProt: Q9NG96 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques combined with 3D Heteronuclear techniques. |
-Sample preparation
Details |
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Sample conditions | pH: 6.9 / Pressure: ambient / Temperature: 296 K | ||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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NMR representative | Selection criteria: fewest violations,lowest energy | |||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry, structures with favorable non-bond energy, structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 50 / Conformers submitted total number: 20 |