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Yorodumi- PDB-1gdt: CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RES... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1gdt | ||||||
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Title | CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE | ||||||
Components |
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Keywords | DNA BINDING PROTEIN/DNA / PROTEIN-DNA COMPLEX / DOUBLE HELIX / OVERHANGING BASE / DNA BINDING PROTEIN-DNA COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3 Å | ||||||
Authors | Yang, W. / Steitz, T.A. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 1995 Title: Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site. Authors: Yang, W. / Steitz, T.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gdt.cif.gz | 118.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gdt.ent.gz | 89.4 KB | Display | PDB format |
PDBx/mmJSON format | 1gdt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gd/1gdt ftp://data.pdbj.org/pub/pdb/validation_reports/gd/1gdt | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 6774.416 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||||||||
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#2: DNA chain | Mass: 3966.597 Da / Num. of mol.: 2 / Source method: obtained synthetically #3: DNA chain | | Mass: 6445.209 Da / Num. of mol.: 1 / Source method: obtained synthetically #4: Protein | Mass: 20394.465 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: STRAINS THAT CONTAIN F EPISOME / Gene: TNPR / Plasmid: MGH285 / Gene (production host): TNPR / Production host: Escherichia coli (E. coli) / Strain (production host): AR120 / References: UniProt: P03012 #5: Water | ChemComp-HOH / | Compound details | RESOLVASE GENE IS PUT UNDER THE CONTROL OF THE LAMBDA PROMOTER AND INDUCED BY NALIDIXIC ACID. | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 54 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 54 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Detector | Date: Apr 1, 1994 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 13255 / % possible obs: 94.2 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.077 |
Reflection | *PLUS Highest resolution: 3 Å / Lowest resolution: 30 Å / % possible obs: 94.2 % / Observed criterion σ(I): 0 |
-Processing
Software |
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Refinement | Resolution: 3→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 26.69 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3 Å / Lowest resolution: 10 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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