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Yorodumi- PDB-1g7t: X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1g7t | ||||||
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Title | X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDPNP | ||||||
Components | TRANSLATION INITIATION FACTOR IF2/EIF5B | ||||||
Keywords | TRANSLATION / translational GTPase | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Methanothermobacter thermautotrophicus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Roll-Mecak, A. / Cao, C. / Dever, T.E. / Burley, S.K. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2000 Title: X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding. Authors: Roll-Mecak, A. / Cao, C. / Dever, T.E. / Burley, S.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1g7t.cif.gz | 132 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1g7t.ent.gz | 99.7 KB | Display | PDB format |
PDBx/mmJSON format | 1g7t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g7/1g7t ftp://data.pdbj.org/pub/pdb/validation_reports/g7/1g7t | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 66340.391 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanothermobacter thermautotrophicus (archaea) Production host: Escherichia coli (E. coli) / References: UniProt: O26359 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-GNP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 65.12 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1M Cacodylate pH 5.6, 18% PEG4000, 0.2M Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.8 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 0.95 Å |
Detector | Type: BRANDEIS / Detector: CCD / Date: Apr 28, 2000 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95 Å / Relative weight: 1 |
Reflection | Resolution: 2→22 Å / Num. all: 61231 / Num. obs: 58931 / % possible obs: 97.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 6 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 17 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 6 % / Rmerge(I) obs: 0.17 / % possible all: 86 |
Reflection shell | *PLUS % possible obs: 86 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→22 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber Details: In REMARK 500, the covalent bonds which deviate from the dictionary values by more than 6RMSD are from residues for which the electron density is very poor beyond (and including) the CB atom.
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2→22 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||
Refine LS restraints | *PLUS
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