+Open data
-Basic information
Entry | Database: PDB / ID: 1eyo | ||||||
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Title | SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA | ||||||
Components | CONOTOXIN TVIIA | ||||||
Keywords | TOXIN / cystine knot motif | ||||||
Function / homology | Conotoxin TVIIAGS / Conotoxin TVIIA/GS family / sodium channel inhibitor activity / : / toxin activity / extracellular region / Conotoxin TVIIA Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Hill, J.M. / Alewood, P.F. / Craik, D.J. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2000 Title: Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure. Authors: Hill, J.M. / Alewood, P.F. / Craik, D.J. #1: Journal: Eur.J.Biochem. / Year: 2000 Title: Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 1. Isolation, characterisation and chemical synthesis Authors: Hill, J.M. / Atkins, A.R. / Loughnan, M.L. / Jones, A. / Adams, D.A. / Martin, R. / Lewis, R.J. / Craik, D.J. / Alewood, P.F. #2: Journal: Structure / Year: 1997 Title: Solution structure of the sodium channel antagonist conotoxin GS: a new molecular caliper for probing sodium channel geometry Authors: Hill, J.M. / Alewood, P.F. / Craik, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eyo.cif.gz | 168.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eyo.ent.gz | 146.1 KB | Display | PDB format |
PDBx/mmJSON format | 1eyo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ey/1eyo ftp://data.pdbj.org/pub/pdb/validation_reports/ey/1eyo | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3221.803 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: THIS PEPTIDE WAS CHEMICALLY SYNTHESIZED. THIS SEQUENCE OCCURS NATURALLY IN CONUS TULIPA (TULIP CONE) References: UniProt: P58923 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 2mM conotoxin TVIIA / Solvent system: 90% H2O/10% D2O or 99.99% D2O |
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Sample conditions | Ionic strength: 0 / pH: 3.0 / Pressure: ambient / Temperature: 283 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: X-PLOR / Version: 3.1 / Developer: Brunger / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 562 interproton distance restraints, and 18 backbone and 9 side chain dihedral angle restraints derived from spin-spin coupling constants. |
NMR representative | Selection criteria: fewest violations,lowest energy |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |