[English] 日本語
Yorodumi- PDB-1e74: NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1 POINT MUTATION VARI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1.0E+74 | ||||||
---|---|---|---|---|---|---|---|
Title | NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1 POINT MUTATION VARIANT R11E | ||||||
Components | ALPHA-CONOTOXIN IM1(R11E) | ||||||
Keywords | PEPTIDE TOXIN / NEUROTOXIN / NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR ANTAGONIST / ALPHA-CONOTOXIN / NMR SOLUTION STRUCTURE | ||||||
Function / homology | Conotoxin, alpha-type, conserved site / Alpha-conotoxin family signature. / host cell postsynaptic membrane / acetylcholine receptor inhibitor activity / ion channel regulator activity / toxin activity / extracellular region / Alpha-conotoxin ImI Function and homology information | ||||||
Biological species | CONUS IMPERIALIS (invertebrata) | ||||||
Method | SOLUTION NMR / TORSION ANGLE DYNAMICS, RESTRAINED ENERGY MINIMISATION | ||||||
Authors | Rogers, J.P. / Luginbuhl, P. / Pemberton, K. / Harty, P. / Wemmer, D.E. / Stevens, R.C. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Structure-Activity Relationships in a Peptidic Alpha7 Nicotinic Acetylcholine Receptor Antagonist Authors: Rogers, J.P. / Luginbuhl, P. / Pemberton, K. / Harty, P. / Wemmer, D.E. / Stevens, R.C. #1: Journal: Biochemistry / Year: 1999 Title: NMR Solution Structure of Alpha-Conotoxin Imi and Comparison to Other Conotoxins Specific for Neuronal Nicotinic Acetylcholine Receptors Authors: Rogers, J.P. / Luginbuhl, P. / Shen, G.S. / Mccabe, R.T. / Stevens, R.C. / Wemmer, D.E. #2: Journal: Mol.Pharmacol. / Year: 1995 Title: Alpha-Conotoxin Imi Exhibits Subtype-Specific Nicotinic Acetylcholine Receptor Blockade: Preferential Inhibition of Homomeric Alpha7 and Alpha9 Receptors Authors: Johnson, D.S. / Martinez, J. / Elgoyhen, A.B. / Heinemann, S.F. / Mcintosh, J.M. #3: Journal: J.Biol.Chem. / Year: 1994 Title: A Nicotinic Acetylcholine Receptor Ligand of Unique Specificity, Alpha-Conotoxin Imi Authors: Mcintosh, J.M. / Yoshikami, D. / Mahe, E. / Nielsen, D.B. / Rivier, J.E. / Gray, W.R. / Olivera, B.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1e74.cif.gz | 60.2 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1e74.ent.gz | 42.8 KB | Display | PDB format |
PDBx/mmJSON format | 1e74.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e7/1e74 ftp://data.pdbj.org/pub/pdb/validation_reports/e7/1e74 | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 1328.521 Da / Num. of mol.: 1 / Mutation: YES / Source method: obtained synthetically Details: SYNTHESIZED USING STANDARD FMOC CHEMISTRY. IM1 SEQUENCE FOUND NATURALLY IN CONUS IMPERIALIS VENOM Source: (synth.) CONUS IMPERIALIS (invertebrata) / References: UniProt: P50983 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||
NMR details | Text: DATA CONSIST OF 61 UPPER LIMITS ON DISTANCES OBTAINED FROM NOE MEASUREMENTS AND 26 ANGLE CONSTRAINTS OBTAINED FROM NOE MEASUREMENTS AND COUPLING CONSTANT MEASUREMENTS. THESE INPUT DATA ARE ALSO ...Text: DATA CONSIST OF 61 UPPER LIMITS ON DISTANCES OBTAINED FROM NOE MEASUREMENTS AND 26 ANGLE CONSTRAINTS OBTAINED FROM NOE MEASUREMENTS AND COUPLING CONSTANT MEASUREMENTS. THESE INPUT DATA ARE ALSO AVAILABLE FROM THE PROTEIN DATA BANK. ONE STEREOSPECIFIC RESONANCE ASSIGNMENT HAS BEEN MADE. TORSION ANGLE DYNAMICS CALCULATIONS WERE PERFORMED WITH THE PROGRAM DYANA (P. GUNTERT, C. MUMENTHALER, K. WUTHRICH, J. MOL. BIOL. (1997) VOL. 273, 283-298). FOR THE RESTRAINED ENERGY MINIMIZATION THE PROGRAM OPAL (P. LUGINBUHL, P. GUNTERT, M. BILLETER, K. WUTHRICH, J. BIOMOL. NMR (1996) VOL. 8, 136-146) WAS USED. DEPOSITED COORDINATES ARE THOSE OF CONFORMERS 1-20 IN THE PAPER CITED ON *JRNL* RECORDS ABOVE. NO VIOLATIONS OF DISTANCE CONSTRAINTS FROM NOES EXCEED 0.10 ANGSTROMS, AND NO VIOLATIONS OF ANGLE CONSTRAINTS EXCEED 2.0 DEGREES. REPRESENTATIVE CONFORMER HAS THE SMALLEST RMSD TO THE MEAN STRUCTURE UPON SUPERPOSITION OF THE BACKBONE ATOMS N, CA, AND C' OF RESIDUES 2-11. |
-Sample preparation
Sample conditions | pH: 3.1 / Temperature: 298 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 400 MHz |
---|
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: TORSION ANGLE DYNAMICS, RESTRAINED ENERGY MINIMISATION Software ordinal: 1 Details: FOR THE PRESENT STRUCTURES THE NMR DISTANCE CONSTRAINTS WERE WEIGHTED SUCH THAT A VIOLATION OF AN UPPER DISTANCE LIMIT OF 0.1 ANGSTROM CORRESPONDS TO AN ENERGY OF KT/2. THE CONSTRAINTS ON ...Details: FOR THE PRESENT STRUCTURES THE NMR DISTANCE CONSTRAINTS WERE WEIGHTED SUCH THAT A VIOLATION OF AN UPPER DISTANCE LIMIT OF 0.1 ANGSTROM CORRESPONDS TO AN ENERGY OF KT/2. THE CONSTRAINTS ON DIHEDRAL ANGLES RESULTING FROM MEASUREMENTS OF VICINAL COUPLING CONSTANTS WERE WEIGHTED SUCH THAT A VIOLATION OF 2.5 DEGREES CORRESPONDS TO AN ENERGY OF KT/2. | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST RESIDUAL TARGET FUNCTION Conformers calculated total number: 40 / Conformers submitted total number: 20 |