+Open data
-Basic information
Entry | Database: PDB / ID: 1im1 | ||||||
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Title | NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1, 20 STRUCTURES | ||||||
Components | ALPHA-CONOTOXIN IM1 | ||||||
Keywords | PEPTIDE TOXIN / NEUROTOXIN / NICOTINIC ACETYLCHOLINE RECEPTOR ANTAGONIST / ALPHA-CONOTOXIN | ||||||
Function / homology | Conotoxin, alpha-type, conserved site / Alpha-conotoxin family signature. / host cell postsynaptic membrane / acetylcholine receptor inhibitor activity / ion channel regulator activity / toxin activity / extracellular region / Alpha-conotoxin ImI Function and homology information | ||||||
Biological species | Conus imperialis (invertebrata) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, RESTRAINED ENERGY REFINEMENT | ||||||
Authors | Rogers, J.P. / Luginbuhl, P. / Shen, G.S. / Mccabe, R.T. / Stevens, R.C. / Wemmer, D.E. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: NMR solution structure of alpha-conotoxin ImI and comparison to other conotoxins specific for neuronal nicotinic acetylcholine receptors. Authors: Rogers, J.P. / Luginbuhl, P. / Shen, G.S. / McCabe, R.T. / Stevens, R.C. / Wemmer, D.E. #1: Journal: Mol.Pharmacol. / Year: 1995 Title: Alpha-Conotoxin Imi Exhibits Subtype-Specific Nicotinic Acetylcholine Receptor Blockade: Preferential Inhibition of Homomeric Alpha 7 and Alpha 9 Receptors Authors: Johnson, D.S. / Martinez, J. / Elgoyhen, A.B. / Heinemann, S.F. / Mcintosh, J.M. #2: Journal: J.Biol.Chem. / Year: 1994 Title: A Nicotinic Acetylcholine Receptor Ligand of Unique Specificity, Alpha-Conotoxin Imi Authors: Mcintosh, J.M. / Yoshikami, D. / Mahe, E. / Nielsen, D.B. / Rivier, J.E. / Gray, W.R. / Olivera, B.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1im1.cif.gz | 60.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1im1.ent.gz | 45 KB | Display | PDB format |
PDBx/mmJSON format | 1im1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/im/1im1 ftp://data.pdbj.org/pub/pdb/validation_reports/im/1im1 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1358.594 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: NEUROTOXIN / Source: (gene. exp.) Conus imperialis (invertebrata) / References: UniProt: P50983 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 90% H2O/10% D2O OR 100% D2O |
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Sample conditions | pH: 3.0 / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: DISTANCE GEOMETRY, RESTRAINED ENERGY REFINEMENT / Software ordinal: 1 Details: FOR THE PRESENT STRUCTURES THE NMR DISTANCE CONSTRAINTS WERE WEIGHTED SUCH THAT A VIOLATION OF AN UPPER DISTANCE LIMIT OF 0.1 ANGSTROM CORRESPONDS TO AN ENERGY OF KT/2. THE CONSTRAINTS ON ...Details: FOR THE PRESENT STRUCTURES THE NMR DISTANCE CONSTRAINTS WERE WEIGHTED SUCH THAT A VIOLATION OF AN UPPER DISTANCE LIMIT OF 0.1 ANGSTROM CORRESPONDS TO AN ENERGY OF KT/2. THE CONSTRAINTS ON DIHEDRAL ANGLES RESULTING FROM MEASUREMENTS OF VICINAL COUPLING CONSTANTS WERE WEIGHTED SUCH THAT A VIOLATION OF 2.5 DEGREES CORRESPONDS TO AN ENERGY OF KT/2. | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST RESIDUAL TARGET FUNCTION Conformers calculated total number: 20 / Conformers submitted total number: 20 |