+Open data
-Basic information
Entry | Database: PDB / ID: 1cwn | ||||||
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Title | CRYSTAL STRUCTURE OF PORCINE ALDEHYDE REDUCTASE HOLOENZYME | ||||||
Components | ALDEHYDE REDUCTASE | ||||||
Keywords | OXIDOREDUCTASE / TIM-BARREL / NADP | ||||||
Function / homology | Function and homology information glucuronate reductase / glucuronolactone reductase / glucuronolactone reductase activity / S-nitrosoglutathione reductase (NADPH) activity / Oxidoreductases; Acting on NADH or NADPH / methylglyoxal reductase (NADPH) (acetol producing) activity / alcohol dehydrogenase (NADP+) / D-glucuronate catabolic process / aldehyde catabolic process / cellular detoxification of aldehyde ...glucuronate reductase / glucuronolactone reductase / glucuronolactone reductase activity / S-nitrosoglutathione reductase (NADPH) activity / Oxidoreductases; Acting on NADH or NADPH / methylglyoxal reductase (NADPH) (acetol producing) activity / alcohol dehydrogenase (NADP+) / D-glucuronate catabolic process / aldehyde catabolic process / cellular detoxification of aldehyde / L-glucuronate reductase activity / glycerol dehydrogenase (NADP+) activity / D/L-glyceraldehyde reductase / allyl-alcohol dehydrogenase / allyl-alcohol dehydrogenase activity / L-ascorbic acid biosynthetic process / aldose reductase (NADPH) activity / lipid metabolic process / apical plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2 Å | ||||||
Authors | El-Kabbani, O. | ||||||
Citation | Journal: Protein Pept.Lett. / Year: 1996 Title: Crystal structure of porcine aldehyde reductase at 2.0 angstrom resolution: Modeling an inhibitor in the active site of the enzyme. Authors: ElKabbani, O. / Carper, D.A. / McGowan, M.H. / Ginell, S.L. #1: Journal: Nat.Struct.Biol. / Year: 1995 Title: Structure of Porcine Aldehyde Reductase Holoenzyme Authors: El-Kabbani, O. / Judge, K. / Ginell, S.L. / Myles, D.A. / Delucas, L.J. / Flynn, T.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cwn.cif.gz | 25.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cwn.ent.gz | 12.4 KB | Display | PDB format |
PDBx/mmJSON format | 1cwn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1cwn_validation.pdf.gz | 387.6 KB | Display | wwPDB validaton report |
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Full document | 1cwn_full_validation.pdf.gz | 388.2 KB | Display | |
Data in XML | 1cwn_validation.xml.gz | 1.6 KB | Display | |
Data in CIF | 1cwn_validation.cif.gz | 4.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cw/1cwn ftp://data.pdbj.org/pub/pdb/validation_reports/cw/1cwn | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36452.691 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: KIDNEY / Tissue: MEDULLA, CORTEX / References: UniProt: P50578, alcohol dehydrogenase (NADP+) |
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#2: Chemical | ChemComp-NAP / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 45 % |
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-Data collection
Diffraction | Mean temperature: 108 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X8C / Wavelength: 1.00615 |
Detector | Detector: CCD / Date: Jun 15, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.00615 Å / Relative weight: 1 |
Reflection | Num. obs: 21648 / % possible obs: 92 % / Observed criterion σ(I): 0 / Redundancy: 1.9 % / Rmerge(I) obs: 0.03 |
-Processing
Software |
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Refinement | Resolution: 2→6 Å / σ(F): 4 /
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Displacement parameters | Biso mean: 16.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.22 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→6 Å
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Refine LS restraints |
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