+Open data
-Basic information
Entry | Database: PDB / ID: 1bsl | ||||||
---|---|---|---|---|---|---|---|
Title | STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN | ||||||
Components | BACTERIAL LUCIFERASE | ||||||
Keywords | FLAVOPROTEIN / PHOTOPROTEIN / LUMINESCENCE / OXIDOREDUCTASE / MONOOXYGENASE / FMN | ||||||
Function / homology | Function and homology information bacterial luciferase / alkanal monooxygenase (FMN-linked) activity / bioluminescence / cytosol Similarity search - Function | ||||||
Biological species | Vibrio harveyi (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.95 Å | ||||||
Authors | Rayment, I. / Holden, H.M. / Thoden, J.B. / Baldwin, T.O. | ||||||
Citation | Journal: Protein Sci. / Year: 1997 Title: Structure of the beta 2 homodimer of bacterial luciferase from Vibrio harveyi: X-ray analysis of a kinetic protein folding trap. Authors: Thoden, J.B. / Holden, H.M. / Fisher, A.J. / Sinclair, J.F. / Wesenberg, G. / Baldwin, T.O. / Rayment, I. #1: Journal: J.Biol.Chem. / Year: 1996 Title: The 1.5-A Resolution Crystal Structure of Bacterial Luciferase in Low Salt Conditions Authors: Fisher, A.J. / Thompson, T.B. / Thoden, J.B. / Baldwin, T.O. / Rayment, I. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1bsl.cif.gz | 160.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1bsl.ent.gz | 126.5 KB | Display | PDB format |
PDBx/mmJSON format | 1bsl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bsl_validation.pdf.gz | 379.5 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1bsl_full_validation.pdf.gz | 419.4 KB | Display | |
Data in XML | 1bsl_validation.xml.gz | 18.9 KB | Display | |
Data in CIF | 1bsl_validation.cif.gz | 33.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bs/1bsl ftp://data.pdbj.org/pub/pdb/validation_reports/bs/1bsl | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 36384.684 Da / Num. of mol.: 2 / Fragment: BETA SUBUNIT Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vibrio harveyi (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P07739, bacterial luciferase #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.98 % | |||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Wavelength: 1.5418 |
---|---|
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Jun 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 55442 / % possible obs: 93.7 % / Redundancy: 2.2 % / Rmerge(I) obs: 0.041 |
Reflection | *PLUS Highest resolution: 1.95 Å / Lowest resolution: 30 Å / Num. measured all: 125700 |
Reflection shell | *PLUS Highest resolution: 1.95 Å / % possible obs: 85.1 % / Num. unique obs: 6873 / Num. measured obs: 9563 / Rmerge(I) obs: 0.18 |
-Processing
Software |
| ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.95→30 Å /
| ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→30 Å
| ||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||
Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.188 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|