+Open data
-Basic information
Entry | Database: PDB / ID: 1bg1 | ||||||
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Title | TRANSCRIPTION FACTOR STAT3B/DNA COMPLEX | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / TRANSCRIPTION FACTOR / PROTEIN-DNA COMPLEX / CYTOKINE ACTIVATION / COMPLEX (TRANSCRIPTION FACTOR-DNA) / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | Function and homology information STAT3 nuclear events downstream of ALK signaling / Interleukin-10 signaling / MET activates STAT3 / Interleukin-37 signaling / Signaling by ALK / PTK6 Activates STAT3 / Interleukin-21 signaling / RNA sequestering activity / Interleukin-9 signaling / Interleukin-4 and Interleukin-13 signaling ...STAT3 nuclear events downstream of ALK signaling / Interleukin-10 signaling / MET activates STAT3 / Interleukin-37 signaling / Signaling by ALK / PTK6 Activates STAT3 / Interleukin-21 signaling / RNA sequestering activity / Interleukin-9 signaling / Interleukin-4 and Interleukin-13 signaling / Interleukin-15 signaling / Interleukin-20 family signaling / Interleukin-27 signaling / Interleukin-23 signaling / retinal rod cell differentiation / Interleukin-6 signaling / radial glial cell differentiation / Interleukin-35 Signalling / Downstream signal transduction / negative regulation of primary miRNA processing / Interleukin-7 signaling / Signaling by SCF-KIT / negative regulation of neuron migration / leptin-mediated signaling pathway / T-helper 17 type immune response / PKR-mediated signaling / negative regulation of hydrogen peroxide biosynthetic process / negative regulation of inflammatory response to wounding / primary miRNA binding / response to leptin / interleukin-11-mediated signaling pathway / CCR5 chemokine receptor binding / sexual reproduction / regulation of feeding behavior / T-helper 17 cell lineage commitment / cellular response to interleukin-17 / interleukin-9-mediated signaling pathway / regulation of cellular response to hypoxia / nuclear glucocorticoid receptor binding / interleukin-2-mediated signaling pathway / positive regulation of growth factor dependent skeletal muscle satellite cell proliferation / acetylation-dependent protein binding / negative regulation of stem cell differentiation / interleukin-15-mediated signaling pathway / cellular response to leptin stimulus / astrocyte differentiation / postsynapse to nucleus signaling pathway / positive regulation of cytokine production involved in inflammatory response / negative regulation of glycolytic process / regulation of mitochondrial membrane permeability / positive regulation of vascular endothelial cell proliferation / eating behavior / temperature homeostasis / interleukin-6-mediated signaling pathway / positive regulation of ATP biosynthetic process / positive regulation of Notch signaling pathway / cellular response to cytokine stimulus / somatic stem cell population maintenance / cell surface receptor signaling pathway via JAK-STAT / positive regulation of interleukin-10 production / cellular response to organic cyclic compound / regulation of multicellular organism growth / positive regulation of vascular endothelial growth factor production / growth hormone receptor signaling pathway via JAK-STAT / signaling adaptor activity / energy homeostasis / phosphatidylinositol 3-kinase/protein kinase B signal transduction / phosphorylation / negative regulation of autophagy / transforming growth factor beta receptor signaling pathway / positive regulation of erythrocyte differentiation / positive regulation of miRNA transcription / positive regulation of interleukin-1 beta production / response to cytokine / acute-phase response / positive regulation of interleukin-8 production / mRNA transcription by RNA polymerase II / response to ischemia / negative regulation of inflammatory response / modulation of chemical synaptic transmission / Schaffer collateral - CA1 synapse / chromatin DNA binding / RNA polymerase II transcription regulator complex / response to peptide hormone / nuclear receptor activity / defense response / cytokine-mediated signaling pathway / positive regulation of interleukin-6 production / protein import into nucleus / positive regulation of angiogenesis / positive regulation of tumor necrosis factor production / glucose homeostasis / response to estradiol / regulation of cell population proliferation / gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / protein phosphatase binding / DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.25 Å | ||||||
Authors | Becker, S. / Groner, B. / Muller, C.W. | ||||||
Citation | Journal: Nature / Year: 1998 Title: Three-dimensional structure of the Stat3beta homodimer bound to DNA. Authors: Becker, S. / Groner, B. / Muller, C.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bg1.cif.gz | 135.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bg1.ent.gz | 107.5 KB | Display | PDB format |
PDBx/mmJSON format | 1bg1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bg1_validation.pdf.gz | 380 KB | Display | wwPDB validaton report |
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Full document | 1bg1_full_validation.pdf.gz | 398.1 KB | Display | |
Data in XML | 1bg1_validation.xml.gz | 14.5 KB | Display | |
Data in CIF | 1bg1_validation.cif.gz | 22.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bg/1bg1 ftp://data.pdbj.org/pub/pdb/validation_reports/bg/1bg1 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 5441.540 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: 18-MER CHEMICALLY SYNTHESIZED, ACTIVE FORM IS 17-MER DUPLEX WITH OVERHANGING ENDS |
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#2: Protein | Mass: 68215.109 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P42227 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.07 Å3/Da / Density % sol: 69.77 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: protein-DNA solution was mixed with an equal volume of reservoir solution | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID2 / Wavelength: 0.987 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 15, 1997 / Details: MIRRORS |
Radiation | Monochromator: SI 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→20 Å / Num. all: 56005 / Num. obs: 56005 / % possible obs: 99.4 % / Redundancy: 4.9 % / Rmerge(I) obs: 0.053 / Net I/σ(I): 16.4 |
Reflection shell | Resolution: 2.25→2.33 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.3 / % possible all: 99.3 |
Reflection | *PLUS Num. measured all: 273455 |
Reflection shell | *PLUS % possible obs: 99.3 % |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.25→20 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.25→20 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.246 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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