+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 5wua | |||||||||
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タイトル | Structure of a Pancreatic ATP-sensitive Potassium Channel | |||||||||
要素 |
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キーワード | TRANSPORT PROTEIN / KATP / channel / ABC transporter / Kir | |||||||||
機能・相同性 | 機能・相同性情報 ATP sensitive Potassium channels / response to resveratrol / ATP-activated inward rectifier potassium channel activity / inward rectifying potassium channel / cell body fiber / Regulation of insulin secretion / sulfonylurea receptor activity / ventricular cardiac muscle tissue development / CAMKK-AMPK signaling cascade / ABC-family proteins mediated transport ...ATP sensitive Potassium channels / response to resveratrol / ATP-activated inward rectifier potassium channel activity / inward rectifying potassium channel / cell body fiber / Regulation of insulin secretion / sulfonylurea receptor activity / ventricular cardiac muscle tissue development / CAMKK-AMPK signaling cascade / ABC-family proteins mediated transport / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / response to stress / Ion homeostasis / inward rectifier potassium channel activity / ATPase-coupled monoatomic cation transmembrane transporter activity / nervous system process / regulation of monoatomic ion transmembrane transport / inorganic cation transmembrane transport / ankyrin binding / response to ATP / response to testosterone / potassium ion import across plasma membrane / action potential / potassium ion binding / intercalated disc / axolemma / ABC-type transporter activity / cellular response to nutrient levels / negative regulation of insulin secretion / T-tubule / heat shock protein binding / positive regulation of protein localization to plasma membrane / potassium ion transport / acrosomal vesicle / response to ischemia / determination of adult lifespan / cellular response to glucose stimulus / sarcolemma / cellular response to nicotine / glucose metabolic process / nuclear envelope / response to estradiol / cellular response to tumor necrosis factor / presynaptic membrane / transmembrane transporter binding / endosome / response to hypoxia / response to xenobiotic stimulus / neuronal cell body / glutamatergic synapse / apoptotic process / ATP hydrolysis activity / ATP binding / plasma membrane / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Mus musculus (ハツカネズミ) synthetic construct (人工物) Mesocricetus auratus (ネズミ) | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.6 Å | |||||||||
データ登録者 | Li, N. / Wu, J.-X. / Chen, L. / Gao, N. | |||||||||
資金援助 | 中国, 2件
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引用 | ジャーナル: Cell / 年: 2017 タイトル: Structure of a Pancreatic ATP-Sensitive Potassium Channel. 著者: Ningning Li / Jing-Xiang Wu / Dian Ding / Jiaxuan Cheng / Ning Gao / Lei Chen / 要旨: ATP-sensitive potassium channels (K) couple intracellular ATP levels with membrane excitability. These channels play crucial roles in many essential physiological processes and have been implicated ...ATP-sensitive potassium channels (K) couple intracellular ATP levels with membrane excitability. These channels play crucial roles in many essential physiological processes and have been implicated extensively in a spectrum of metabolic diseases and disorders. To gain insight into the mechanism of K, we elucidated the structure of a hetero-octameric pancreatic K channel in complex with a non-competitive inhibitor glibenclamide by single-particle cryoelectron microscopy to 5.6-Å resolution. The structure shows that four SUR1 regulatory subunits locate peripherally and dock onto the central Kir6.2 channel tetramer through the SUR1 TMD0-L0 fragment. Glibenclamide-bound SUR1 uses TMD0-L0 fragment to stabilize Kir6.2 channel in a closed conformation. In another structural population, a putative co-purified phosphatidylinositol 4,5-bisphosphate (PIP) molecule uncouples Kir6.2 from glibenclamide-bound SUR1. These structural observations suggest a molecular mechanism for K regulation by anti-diabetic sulfonylurea drugs, intracellular adenosine nucleotide concentrations, and PIP lipid. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 5wua.cif.gz | 954.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb5wua.ent.gz | 701.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 5wua.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 5wua_validation.pdf.gz | 1.1 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 5wua_full_validation.pdf.gz | 1.2 MB | 表示 | |
XML形式データ | 5wua_validation.xml.gz | 171.9 KB | 表示 | |
CIF形式データ | 5wua_validation.cif.gz | 260.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/wu/5wua ftp://data.pdbj.org/pub/pdb/validation_reports/wu/5wua | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
#1: タンパク質 | 分子量: 76339.242 Da / 分子数: 4 / 由来タイプ: 組換発現 詳細: ATP-sensitive inward rectifier potassium channel 11 with C-terminal synthetic superfolder GFP and affinity tags added 由来: (組換発現) Mus musculus (ハツカネズミ), (組換発現) synthetic construct (人工物) 遺伝子: Kcnj11 / 細胞株 (発現宿主): HEK / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: Q61743 #2: タンパク質 | 分子量: 177296.578 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Mesocricetus auratus (ネズミ) / 細胞株 (発現宿主): HEK / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: A0A1S4NYG1*PLUS |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 |
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分子量 | 値: 880 kDa/nm / 実験値: NO | ||||||||||||||||||||||||
由来(天然) |
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由来(組換発現) |
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緩衝液 | pH: 7.5 | ||||||||||||||||||||||||
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 40 e/Å2 / 検出モード: INTEGRATING フィルム・検出器のモデル: FEI FALCON II (4k x 4k) |
-解析
EMソフトウェア | 名称: relion / バージョン: 2 / カテゴリ: 3次元再構成 |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
対称性 | 点対称性: C4 (4回回転対称) |
3次元再構成 | 解像度: 5.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 34500 / 対称性のタイプ: POINT |