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-基本情報
登録情報 | データベース: PDB / ID: 5jco | ||||||||||||
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タイトル | Structure and dynamics of single-isoform recombinant neuronal human tubulin | ||||||||||||
要素 |
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キーワード | STRUCTURAL PROTEIN (タンパク質) / microtubules (微小管) / tubulin (チューブリン) / single isoform / recombinant / dynamic instability | ||||||||||||
機能・相同性 | 機能・相同性情報 netrin receptor binding / Post-chaperonin tubulin folding pathway / axonemal microtubule / Carboxyterminal post-translational modifications of tubulin / dorsal root ganglion development / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / organelle transport along microtubule / glial cell differentiation / cytoskeleton-dependent intracellular transport ...netrin receptor binding / Post-chaperonin tubulin folding pathway / axonemal microtubule / Carboxyterminal post-translational modifications of tubulin / dorsal root ganglion development / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / organelle transport along microtubule / glial cell differentiation / cytoskeleton-dependent intracellular transport / forebrain morphogenesis / Intraflagellar transport / Sealing of the nuclear envelope (NE) by ESCRT-III / neuron projection arborization / Gap junction assembly / Formation of tubulin folding intermediates by CCT/TriC / cerebellar cortex morphogenesis / dentate gyrus development / COPI-independent Golgi-to-ER retrograde traffic / pyramidal neuron differentiation / Prefoldin mediated transfer of substrate to CCT/TriC / Kinesins / Assembly and cell surface presentation of NMDA receptors / centrosome cycle / COPI-dependent Golgi-to-ER retrograde traffic / motor behavior / response to L-glutamate / smoothened signaling pathway / regulation of synapse organization / locomotory exploration behavior / 細胞結合 / startle response / 微小管 / Recycling pathway of L1 / RHO GTPases activate IQGAPs / Hedgehog 'off' state / response to tumor necrosis factor / microtubule-based process / COPI-mediated anterograde transport / Activation of AMPK downstream of NMDARs / response to mechanical stimulus / Mitotic Prometaphase / homeostasis of number of cells within a tissue / EML4 and NUDC in mitotic spindle formation / condensed chromosome / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Resolution of Sister Chromatid Cohesion / Recruitment of NuMA to mitotic centrosomes / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / Anchoring of the basal body to the plasma membrane / MHC class II antigen presentation / cellular response to calcium ion / adult locomotory behavior / AURKA Activation by TPX2 / filopodium / cell periphery / RHO GTPases Activate Formins / Translocation of SLC2A4 (GLUT4) to the plasma membrane / 軸索誘導 / synapse organization / peptide binding / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / intracellular protein transport / neuron migration / neuromuscular junction / visual learning / PKR-mediated signaling / structural constituent of cytoskeleton / cytoplasmic ribonucleoprotein granule / 紡錘体 / cerebral cortex development / 記憶 / microtubule cytoskeleton organization / recycling endosome / Aggrephagy / HCMV Early Events / Separation of Sister Chromatids / The role of GTSE1 in G2/M progression after G2 checkpoint / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / lamellipodium / mitotic cell cycle / 遺伝子発現 / 成長円錐 / neuron apoptotic process / 微小管 / hydrolase activity / protein heterodimerization activity / 細胞分裂 / 神経繊維 / GTPase activity / 樹状突起 / neuronal cell body / protein-containing complex binding / GTP binding / structural molecule activity / extracellular exosome / identical protein binding 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||
手法 | 電子顕微鏡法 / らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 4 Å | ||||||||||||
データ登録者 | Vemu, A. / Atherton, J. / Spector, J.O. / Szyk, A. / Moores, C.A. / Roll-Mecak, A. | ||||||||||||
資金援助 | 英国, 米国, 3件
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引用 | ジャーナル: J Biol Chem / 年: 2016 タイトル: Structure and Dynamics of Single-isoform Recombinant Neuronal Human Tubulin. 著者: Annapurna Vemu / Joseph Atherton / Jeffrey O Spector / Agnieszka Szyk / Carolyn A Moores / Antonina Roll-Mecak / 要旨: Microtubules are polymers that cycle stochastically between polymerization and depolymerization, i.e. they exhibit "dynamic instability." This behavior is crucial for cell division, motility, and ...Microtubules are polymers that cycle stochastically between polymerization and depolymerization, i.e. they exhibit "dynamic instability." This behavior is crucial for cell division, motility, and differentiation. Although studies in the last decade have made fundamental breakthroughs in our understanding of how cellular effectors modulate microtubule dynamics, analysis of the relationship between tubulin sequence, structure, and dynamics has been held back by a lack of dynamics measurements with and structural characterization of homogeneous isotypically pure engineered tubulin. Here, we report for the first time the cryo-EM structure and in vitro dynamics parameters of recombinant isotypically pure human tubulin. α1A/βIII is a purely neuronal tubulin isoform. The 4.2-Å structure of post-translationally unmodified human α1A/βIII microtubules shows overall similarity to that of heterogeneous brain microtubules, but it is distinguished by subtle differences at polymerization interfaces, which are hot spots for sequence divergence between tubulin isoforms. In vitro dynamics assays show that, like mosaic brain microtubules, recombinant homogeneous microtubules undergo dynamic instability, but they polymerize slower and have fewer catastrophes. Interestingly, we find that epitaxial growth of α1A/βIII microtubules from heterogeneous brain seeds is inefficient but can be fully rescued by incorporating as little as 5% of brain tubulin into the homogeneous α1A/βIII lattice. Our study establishes a system to examine the structure and dynamics of mammalian microtubules with well defined tubulin species and is a first and necessary step toward uncovering how tubulin genetic and chemical diversity is exploited to modulate intrinsic microtubule dynamics. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 5jco.cif.gz | 994.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb5jco.ent.gz | 818.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 5jco.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jc/5jco ftp://data.pdbj.org/pub/pdb/validation_reports/jc/5jco | HTTPS FTP |
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-関連構造データ
関連構造データ | 8150MC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | |
電子顕微鏡画像生データ | EMPIAR-10071 (タイトル: Structure and Dynamics of Single-isoform Recombinant Neuronal Human Tubulin Data size: 487.7 Data #1: Unaligned frame stacks of GMPCPP-bound alpha1a beta3 recombinant tubulin microtubules [micrographs - multiframe]) |
-リンク
-集合体
登録構造単位 |
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非結晶学的対称性 (NCS) | NCSドメイン:
NCSドメイン領域: Component-ID: 0 / Beg auth comp-ID: MET / Beg label comp-ID: MET / Refine code: 0
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