+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2n27 | ||||||
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タイトル | Competitive inhibition of TRPV1 calmodulin interaction by vanilloids | ||||||
要素 | Calmodulinカルモジュリン | ||||||
キーワード | METAL BINDING PROTEIN / calmodulin (カルモジュリン) / capsaicin (カプサイシン) | ||||||
機能・相同性 | 機能・相同性情報 : / establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / CAM型光合成 / Cam-PDE 1 activation / Sodium/Calcium exchangers / Calmodulin induced events / Reduction of cytosolic Ca++ levels / Activation of Ca-permeable Kainate Receptor / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde ...: / establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / CAM型光合成 / Cam-PDE 1 activation / Sodium/Calcium exchangers / Calmodulin induced events / Reduction of cytosolic Ca++ levels / Activation of Ca-permeable Kainate Receptor / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / regulation of synaptic vesicle endocytosis / Loss of phosphorylation of MECP2 at T308 / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation / negative regulation of high voltage-gated calcium channel activity / regulation of synaptic vesicle exocytosis / CaMK IV-mediated phosphorylation of CREB / Glycogen breakdown (glycogenolysis) / negative regulation of calcium ion export across plasma membrane / organelle localization by membrane tethering / Activation of RAC1 downstream of NMDARs / regulation of cardiac muscle cell action potential / mitochondrion-endoplasmic reticulum membrane tethering / CLEC7A (Dectin-1) induces NFAT activation / autophagosome membrane docking / response to corticosterone / positive regulation of ryanodine-sensitive calcium-release channel activity / Negative regulation of NMDA receptor-mediated neuronal transmission / nitric-oxide synthase binding / regulation of cell communication by electrical coupling involved in cardiac conduction / Unblocking of NMDA receptors, glutamate binding and activation / negative regulation of peptidyl-threonine phosphorylation / Synthesis of IP3 and IP4 in the cytosol / Phase 0 - rapid depolarisation / protein phosphatase activator activity / RHO GTPases activate PAKs / positive regulation of cyclic-nucleotide phosphodiesterase activity / positive regulation of phosphoprotein phosphatase activity / 長期増強 / Ion transport by P-type ATPases / Uptake and function of anthrax toxins / adenylate cyclase binding / Calcineurin activates NFAT / Regulation of MECP2 expression and activity / catalytic complex / DARPP-32 events / detection of calcium ion / negative regulation of ryanodine-sensitive calcium-release channel activity / Smooth Muscle Contraction / RHO GTPases activate IQGAPs / regulation of cardiac muscle contraction / calcium channel inhibitor activity / cellular response to interferon-beta / positive regulation of DNA binding / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Protein methylation / enzyme regulator activity / voltage-gated potassium channel complex / phosphatidylinositol 3-kinase binding / Activation of AMPK downstream of NMDARs / eNOS activation / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / regulation of calcium-mediated signaling / positive regulation of protein dephosphorylation / titin binding / regulation of ryanodine-sensitive calcium-release channel activity / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / Ion homeostasis / positive regulation of protein autophosphorylation / sperm midpiece / response to amphetamine / calcium channel complex / activation of adenylate cyclase activity / substantia nigra development / adenylate cyclase activator activity / Ras activation upon Ca2+ influx through NMDA receptor / regulation of heart rate / nitric-oxide synthase regulator activity / protein serine/threonine kinase activator activity / sarcomere / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / VEGFR2 mediated vascular permeability / positive regulation of peptidyl-threonine phosphorylation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / VEGFR2 mediated cell proliferation / regulation of cytokinesis / positive regulation of nitric-oxide synthase activity / Translocation of SLC2A4 (GLUT4) to the plasma membrane / spindle microtubule / ミトコンドリア / RAF activation / positive regulation of receptor signaling pathway via JAK-STAT / positive regulation of protein serine/threonine kinase activity / Transcriptional activation of mitochondrial biogenesis / Stimuli-sensing channels / synaptic vesicle membrane / 紡錘体 / cellular response to type II interferon / response to calcium ion 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / molecular dynamics | ||||||
データ登録者 | Hetenyi, A. / Nemeth, L. / Weber, E. / Szakonyi, G. / Winter, Z. / Josvay, K. / Bartus, E. / Olah, Z. / Martinek, T.A. | ||||||
引用 | ジャーナル: Febs Lett. / 年: 2016 タイトル: Competitive inhibition of TRPV1-calmodulin interaction by vanilloids. 著者: Hetenyi, A. / Nemeth, L. / Weber, E. / Szakonyi, G. / Winter, Z. / Josvay, K. / Bartus, E. / Olah, Z. / Martinek, T.A. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2n27.cif.gz | 895.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2n27.ent.gz | 753.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2n27.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n2/2n27 ftp://data.pdbj.org/pub/pdb/validation_reports/n2/2n27 | HTTPS FTP |
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-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 16721.350 Da / 分子数: 1 / 断片: UNP residues 2-149 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: pET28a(+) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P62158, UniProt: P0DP23*PLUS | ||
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#2: 化合物 | ChemComp-CA / #3: 化合物 | ChemComp-4DY / ( | |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | イオン強度: 30 / pH: 7.0 / 圧: ambient / 温度: 303 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker Avance / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: molecular dynamics / ソフトェア番号: 1 | ||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 519 / NOE intraresidue total count: 82 / NOE long range total count: 50 / NOE medium range total count: 57 / NOE sequential total count: 90 / Protein chi angle constraints total count: 0 / Protein phi angle constraints total count: 103 / Protein psi angle constraints total count: 103 | ||||||||||||||||||||||||||||
代表構造 | 選択基準: fewest violations | ||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: back calculated data agree with experimental NOESY spectrum 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |