+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2fn2 | ||||||
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タイトル | SOLUTION NMR STRUCTURE OF THE GLYCOSYLATED SECOND TYPE TWO MODULE OF FIBRONECTIN, 20 STRUCTURES | ||||||
要素 | FIBRONECTINフィブロネクチン | ||||||
キーワード | GLYCOPROTEIN (糖タンパク質) / FIBRONECTIN (フィブロネクチン) / TYPE TWO MODULE / GLYCOSYLATED PROTEIN (グリコシル化) / COLLAGEN (コラーゲン) | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking ...negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking / integrin activation / ALK mutants bind TKIs / cell-substrate junction assembly / biological process involved in interaction with symbiont / Molecules associated with elastic fibres / proteoglycan binding / extracellular matrix structural constituent / MET activates PTK2 signaling / Syndecan interactions / p130Cas linkage to MAPK signaling for integrins / endodermal cell differentiation / GRB2:SOS provides linkage to MAPK signaling for Integrins / Non-integrin membrane-ECM interactions / Signaling by ALK fusions and activated point mutants / 基底膜 / ECM proteoglycans / endoplasmic reticulum-Golgi intermediate compartment / positive regulation of axon extension / Integrin cell surface interactions / Nuclear events stimulated by ALK signaling in cancer / collagen binding / 細胞外マトリックス / Degradation of the extracellular matrix / Integrin signaling / substrate adhesion-dependent cell spreading / cell-matrix adhesion / regulation of ERK1 and ERK2 cascade / platelet alpha granule lumen / integrin-mediated signaling pathway / Post-translational protein phosphorylation / acute-phase response / Cell surface interactions at the vascular wall / regulation of protein phosphorylation / Signaling by high-kinase activity BRAF mutants / wound healing / MAP2K and MAPK activation / response to wounding / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / GPER1 signaling / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / positive regulation of fibroblast proliferation / Signaling by BRAF and RAF1 fusions / integrin binding / Platelet degranulation / heparin binding / nervous system development / heart development / regulation of cell shape / collagen-containing extracellular matrix / 血管新生 / blood microparticle / Interleukin-4 and Interleukin-13 signaling / protease binding / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / 細胞接着 / apical plasma membrane / 小胞体 / signaling receptor binding / positive regulation of cell population proliferation / positive regulation of gene expression / extracellular space / extracellular exosome / extracellular region / identical protein binding / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS | ||||||
データ登録者 | Sticht, H. / Pickford, A.R. / Potts, J.R. / Campbell, I.D. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 1998 タイトル: Solution structure of the glycosylated second type 2 module of fibronectin. 著者: Sticht, H. / Pickford, A.R. / Potts, J.R. / Campbell, I.D. #1: ジャーナル: Structure / 年: 1997 タイトル: Solution Structure of a Type 2 Module from Fibronectin: Implications for the Structure and Function of the Gelatin-Binding Domain 著者: Pickford, A.R. / Potts, J.R. / Bright, J.R. / Phan, I. / Campbell, I.D. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2fn2.cif.gz | 357.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2fn2.ent.gz | 298.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2fn2.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/fn/2fn2 ftp://data.pdbj.org/pub/pdb/validation_reports/fn/2fn2 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 6703.393 Da / 分子数: 1 / 断片: RESIDUES 375 - 433 / 由来タイプ: 組換発現 / 詳細: GLYCOSYLATED SECOND TYPE II MODULE / 由来: (組換発現) Homo sapiens (ヒト) / 細胞内の位置: EXTRACELLULARGlossary of biology / 発現宿主: Pichia pastoris (菌類) / 株 (発現宿主): GS115 (HIS4-) / 参照: UniProt: P02751 |
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#2: 糖 | ChemComp-NAG / |
-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験 | タイプ: TWO-DIMENSIONAL 1H-1H |
-試料調製
詳細 | 内容: H2O/D2O(9:1) OR D2O |
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試料状態 | イオン強度: 0 mM / pH: 4.5 / 圧: 10E+5 PA atm / 温度: 298 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Home-built OMEGA 500 / 製造業者: Home-built / モデル: OMEGA 500 / 磁場強度: 500 MHz |
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-解析
ソフトウェア |
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NMR software |
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精密化 | 手法: SIMULATED ANNEALING, RESTRAINED MOLECULAR DYNAMICS / ソフトェア番号: 1 | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: ENERGY, AGREEMENT WITH EXPERIMENTAL DATA 計算したコンフォーマーの数: 80 / 登録したコンフォーマーの数: 20 |