+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1znj | ||||||
---|---|---|---|---|---|---|---|
タイトル | INSULIN, MONOCLINIC CRYSTAL FORM | ||||||
要素 | (INSULINインスリン) x 2 | ||||||
キーワード | HORMONE (ホルモン) / METABOLIC ROLE / CHEMICAL ACTIVITY (活量) / INSULIN GLUCOSE METABOLISM / DIABETES (糖尿病) | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of dendritic spine maintenance / alpha-beta T cell activation / negative regulation of acute inflammatory response / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / fatty acid homeostasis / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / positive regulation of lipid biosynthetic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / positive regulation of nitric oxide mediated signal transduction / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of lipid catabolic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / negative regulation of reactive oxygen species biosynthetic process / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / Regulation of insulin secretion / positive regulation of nitric-oxide synthase activity / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of protein secretion / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of glucose import / negative regulation of proteolysis / regulation of synaptic plasticity / wound healing / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of neuron projection development / hormone activity / 認識 / Golgi lumen / vasodilation / positive regulation of protein localization to nucleus / glucose metabolic process / regulation of protein localization / glucose homeostasis / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 分子置換 / 解像度: 2 Å | ||||||
データ登録者 | Turkenburg, M.G.W. / Whittingham, J.L. / Turkenburg, J.P. / Dodson, G.G. / Derewenda, U. / Smith, G.D. / Dodson, E.J. / Derewenda, Z.S. / Xiao, B. | ||||||
引用 | ジャーナル: To be Published タイトル: Structure Determination and Refinement of Two Crystal Forms of Native Insulins 著者: Turkenburg, J.P. / Whittingham, J.L. / Derewenda, U. / Derewenda, Z.S. / Dodson, E.J. / Dodson, G.G. / Smith, G.D. / Xiao, B. #1: ジャーナル: Biopolymers / 年: 1992 タイトル: The Structure of a Rhombohedral R6 Insulin Hexamer that Binds Phenol 著者: Smith, G.D. / Dodson, G.G. #2: ジャーナル: Nature / 年: 1989 タイトル: Phenol Stabilizes More Helix in a New Symmetrical Zinc Insulin Hexamer 著者: Derewenda, U. / Derewenda, Z. / Dodson, E.J. / Dodson, G.G. / Reynolds, C.D. / Smith, G.D. / Sparks, C. / Swenson, D. #3: ジャーナル: Daresbury Lab.[Rep.]Dl/Sci/R / 年: 1985 タイトル: Molecular Replacement: The Method and its Problems (in: Molecular Replacement. Proceedings of the Daresbury Study Weekend, 15-16 February, 1985. Compiled by P.A.Machin) 著者: Dodson, E.J. | ||||||
履歴 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1znj.cif.gz | 77.7 KB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb1znj.ent.gz | 61.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1znj.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/zn/1znj ftp://data.pdbj.org/pub/pdb/validation_reports/zn/1znj | HTTPS FTP |
---|
-関連構造データ
関連構造データ | 4insS S: 精密化の開始モデル |
---|---|
類似構造データ |
-リンク
-集合体
登録構造単位 |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
単位格子 |
|
-要素
-タンパク質・ペプチド , 2種, 12分子 ACEGIKBDFHJL
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 6 / 由来タイプ: 組換発現 / 詳細: HEXAMER IN ASYMMETRIC UNIT, MONOCLINIC CRYSTAL FORM / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 6 / 由来タイプ: 組換発現 / 詳細: HEXAMER IN ASYMMETRIC UNIT, MONOCLINIC CRYSTAL FORM / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 |
---|
-非ポリマー , 4種, 342分子
#3: 化合物 | ChemComp-IPH / #4: 化合物 | #5: 化合物 | #6: 水 | ChemComp-HOH / | |
---|
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
---|
-試料調製
結晶 | マシュー密度: 2.43 Å3/Da / 溶媒含有率: 49.45 % |
---|---|
結晶化 | 手法: batch method / pH: 7.44 詳細: BATCH, 1.7MG/ML INSULIN, 0.02 ZINC ACETATE, 0.7% (V/V) PHENOL, 0.34M SODIUM CHLORIDE, pH 7.44, batch method |
-データ収集
回折 | 平均測定温度: 293 K |
---|---|
放射光源 | 由来: 回転陽極 / タイプ: RIGAKU RUH2R / 波長: 1.5418 |
検出器 | タイプ: RIGAKU / 検出器: IMAGE PLATE / 日付: 1992年9月1日 |
放射 | モノクロメーター: GRAPHITE(002) / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2→30 Å / Num. obs: 22429 / % possible obs: 98.6 % / 冗長度: 3.1 % / Rmerge(I) obs: 0.079 / Net I/σ(I): 9.3 |
反射 シェル | 解像度: 2→2.07 Å / 冗長度: 2.5 % / Rmerge(I) obs: 0.227 / Mean I/σ(I) obs: 3.3 / % possible all: 90.1 |
-解析
ソフトウェア |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 構造決定の手法: 分子置換 開始モデル: 2ZINC INSULIN DIMER (PDB ENTRY 4INS) 解像度: 2→8 Å / σ(F): 0
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2→8 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 |
|