+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1bsr | ||||||
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タイトル | BOVINE SEMINAL RIBONUCLEASE STRUCTURE AT 1.9 ANGSTROMS RESOLUTION | ||||||
要素 | BOVINE SEMINAL RIBONUCLEASE | ||||||
キーワード | HYDROLASE(PHOSPHORIC DIESTER / RNA) | ||||||
機能・相同性 | 機能・相同性情報 pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Bos taurus (ウシ) | ||||||
手法 | X線回折 / 解像度: 1.9 Å | ||||||
データ登録者 | Mazzarella, L. | ||||||
引用 | ジャーナル: Acta Crystallogr.,Sect.D / 年: 1993 タイトル: Bovine seminal ribonuclease: structure at 1.9 A resolution. 著者: Mazzarella, L. / Capasso, S. / Demasi, D. / Di Lorenzo, G. / Mattia, C.A. / Zagari, A. #1: ジャーナル: Gazz.Chim.Ital. / 年: 1987 タイトル: Composite Active Sites in Bovine Seminal Ribonuclease 著者: Mazzarella, L. / Mattia, C.A. / Capasso, S. / Di Lorenzo, G. #2: ジャーナル: Biopolymers / 年: 1983 タイトル: Refinement of the Structure of Bovine Seminal Ribonuclease 著者: Capasso, S. / Giordano, F. / Mattia, C.A. / Mazzarella, L. / Zagari, A. #3: ジャーナル: Gazz.Chim.Ital. / 年: 1979 タイトル: Low-Resolution Structure of Bovine Seminal Ribonuclease: A Covalent Dimeric Protein 著者: Capasso, S. / Giordano, F. / Mattia, C.A. / Mazzarella, L. / Zagari, A. | ||||||
履歴 |
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Remark 700 | SHEET THE TWO BETA SHEETS OF EACH CHAIN OF THIS STRUCTURE ARE VERY CLOSE TO THOSE OF RNASE A (5RSA, ...SHEET THE TWO BETA SHEETS OF EACH CHAIN OF THIS STRUCTURE ARE VERY CLOSE TO THOSE OF RNASE A (5RSA, REMARK 5), AND A SIMILAR NOTATION HAS BEEN USED, MODIFIED ONLY TO TAKE INTO ACCOUNT THE PRESENCE OF TWO CHAINS. THEREFORE, DUE TO A BULGE OF RESIDUES 88 - 89, THE FIRST SHEET OF CHAIN A (B), COMPOSED OF 3 STRANDS, CONSISTS OF TWO PARTS DENOTED *S1A* AND *S2A* (*S1B* AND *S2B*), HAVING STRAND 1 AND 3 IDENTICAL. FOR CHAIN A (B), ALSO THE SECOND SHEET, FORMED BY 4 STRANDS, IS COMPOSED BY TWO PARTS DENOTED *S3A* AND *S4A* (*S3B* AND *S4B*), HAVING STRANDS 1, 2 AND 3 IDENTICAL. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1bsr.cif.gz | 62.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1bsr.ent.gz | 46.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1bsr.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1bsr_validation.pdf.gz | 382.2 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1bsr_full_validation.pdf.gz | 383.5 KB | 表示 | |
XML形式データ | 1bsr_validation.xml.gz | 6.4 KB | 表示 | |
CIF形式データ | 1bsr_validation.cif.gz | 10.2 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/bs/1bsr ftp://data.pdbj.org/pub/pdb/validation_reports/bs/1bsr | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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2 |
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単位格子 |
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Atom site foot note | 1: CIS PROLINE - PRO A 93 / 2: CIS PROLINE - PRO A 114 / 3: CIS PROLINE - PRO B 93 / 4: CIS PROLINE - PRO B 114 | ||||||||
Components on special symmetry positions |
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-要素
#1: タンパク質 | 分子量: 13632.640 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Bos taurus (ウシ) / 参照: UniProt: P00669 #2: 化合物 | ChemComp-SO4 / #3: 水 | ChemComp-HOH / | 構成要素の詳細 | FEATURES OF THE STRUCTURE: RESIDUES 1 - 15 OF ONE CHAIN FOLD AGAINST RESIDUES 23 - 124 OF THE OTHER ...FEATURES OF THE STRUCTURE: RESIDUES 1 - 15 OF ONE CHAIN FOLD AGAINST RESIDUES 23 - 124 OF THE OTHER CHAIN. THEREFORE, IN THE DIMER, THE TWO CHAINS PRESENT THEIR N-TERMINI INTERCHANG | 非ポリマーの詳細 | A TOTAL OF 113 WATER MOLECULES, AS WELL AS 7 SULFATE ANIONS, WERE INCLUDED AND REFINED AS PART OF ...A TOTAL OF 113 WATER MOLECULES, AS WELL AS 7 SULFATE ANIONS, WERE INCLUDED AND REFINED AS PART OF THE STRUCTURE. | |
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-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 2.4 Å3/Da / 溶媒含有率: 48.72 % | |||||||||||||||||||||||||
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結晶化 | *PLUS pH: 5.1 / 手法: batch method | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | *PLUS 最高解像度: 1.9 Å / 最低解像度: 9999 Å / Num. obs: 17217 / % possible obs: 90 % / Observed criterion σ(I): 3 / Num. measured all: 59869 / Rmerge(I) obs: 0.067 / Biso Wilson estimate: 9.2 Å2 |
-解析
ソフトウェア |
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精密化 | Rfactor Rwork: 0.177 / Rfactor obs: 0.177 / 最高解像度: 1.9 Å 詳細: A SECOND POSITION HAS BEEN LOCATED AND REFINED FOR THE SIDE CHAINS OF HIS A 119 AND ARG B 71. IN BOTH CHAINS, GLN 60 HAS A CONFORMATION WHICH FALLS OUTSIDE THE ALLOWED REGIONS OF THE ...詳細: A SECOND POSITION HAS BEEN LOCATED AND REFINED FOR THE SIDE CHAINS OF HIS A 119 AND ARG B 71. IN BOTH CHAINS, GLN 60 HAS A CONFORMATION WHICH FALLS OUTSIDE THE ALLOWED REGIONS OF THE RAMACHANDRAN MAP, AS OBSERVED IN RNASE A. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 最高解像度: 1.9 Å
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拘束条件 |
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精密化 | *PLUS 最高解像度: 1.9 Å / 最低解像度: 6 Å / Num. reflection obs: 16492 / σ(I): 3 / Rfactor obs: 0.177 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS タイプ: x_angle_d / Dev ideal: 3.7 |