Staphylococcus aureus phage 80alpha-derived SaPI1 mature capsid. Sharpened map used for model fitting and refinement. Map sharpened with bsoft by application of inverse Bfactor = 800.
試料
ウイルス: Staphylococcus phage 80alpha (ファージ)
タンパク質・ペプチド: Major head protein
キーワード
major capsid protein / HK97-like fold / mature capsid / VIRUS (ウイルス)
機能・相同性
Phage capsid / Phage capsid family / Major capsid protein
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
R01AI083255
米国
引用
ジャーナル: Viruses / 年: 2017 タイトル: Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids. 著者: James L Kizziah / Keith A Manning / Altaira D Dearborn / Erin A Wall / Laura Klenow / Rosanne L L Hill / Michael S Spilman / Scott M Stagg / Gail E Christie / Terje Dokland / 要旨: In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of ...In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. bacteriophage 80α is capable of high frequency mobilization of mobile genetic elements called pathogenicity islands (SaPIs), such as SaPI1. SaPI1 redirects the assembly pathway of 80α to form capsids that are smaller than those normally made by the phage alone. Both CP and SP of 80α are N-terminally processed by a host-encoded protease, Prp. We have analyzed phage mutants that express pre-cleaved or uncleavable versions of CP or SP, and show that the N-terminal sequence in SP is absolutely required for assembly, but does not need to be cleaved in order to produce viable capsids. Mutants with pre-cleaved or uncleavable CP display normal viability. We have used cryo-EM to solve the structures of mature capsids from an 80α mutant expressing uncleavable CP, and from wildtype SaPI1. Comparisons with structures of 80α and SaPI1 procapsids show that capsid maturation involves major conformational changes in CP, consistent with a release of the CP N-arm by SP. The hexamers reorganize during maturation to accommodate the different environments in the 80α and SaPI1 capsids.
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (2)
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マップ
ファイル
ダウンロード / ファイル: emd_7333.map.gz / 形式: CCP4 / 大きさ: 92.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Staphylococcus aureus phage 80alpha-derived SaPI1 mature capsid. Sharpened map used for model fitting and refinement. Map sharpened with bsoft by application of inverse Bfactor = 800.
ボクセルのサイズ
X=Y=Z: 2.048 Å
密度
表面レベル
登録者による: 2.0 / ムービー #1: 2
最小 - 最大
-6.673943 - 10.581618000000001
平均 (標準偏差)
-0.000000003075934 (±1.0)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
289
289
289
Spacing
289
289
289
セル
A=B=C: 591.872 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.048
2.048
2.048
M x/y/z
289
289
289
origin x/y/z
0.000
0.000
0.000
length x/y/z
591.872
591.872
591.872
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
0
0
0
NX/NY/NZ
28
11
56
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
289
289
289
D min/max/mean
-6.674
10.582
-0.000
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添付データ
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試料の構成要素
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全体 : Staphylococcus phage 80alpha
全体
名称: Staphylococcus phage 80alpha (ファージ)
要素
ウイルス: Staphylococcus phage 80alpha (ファージ)
タンパク質・ペプチド: Major head protein
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超分子 #1: Staphylococcus phage 80alpha
超分子
名称: Staphylococcus phage 80alpha / タイプ: virus / ID: 1 / 親要素: 0 / 含まれる分子: all / NCBI-ID: 53369 / 生物種: Staphylococcus phage 80alpha / ウイルスタイプ: VIRION / ウイルス・単離状態: SPECIES / ウイルス・エンベロープ: No / ウイルス・中空状態: No