+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-14722 | |||||||||
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タイトル | Cryo-EM structure of USP1-UAF1 bound to FANCI and mono-ubiquitinated FANCD2 with ML323 (consensus reconstruction) | |||||||||
マップデータ | Globally sharpened map | |||||||||
試料 |
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キーワード | Deubiquitinase / Complex / Enzyme-Substrate / Inhibitor / HYDROLASE | |||||||||
機能・相同性 | 機能・相同性情報 positive regulation of error-prone translesion synthesis / regulation of protein monoubiquitination / Signaling by cytosolic PDGFRA and PDGFRB fusion proteins / regulation of CD40 signaling pathway / regulation of regulatory T cell differentiation / monoubiquitinated protein deubiquitination / double-strand break repair involved in meiotic recombination / homologous chromosome pairing at meiosis / gamete generation / neuronal stem cell population maintenance ...positive regulation of error-prone translesion synthesis / regulation of protein monoubiquitination / Signaling by cytosolic PDGFRA and PDGFRB fusion proteins / regulation of CD40 signaling pathway / regulation of regulatory T cell differentiation / monoubiquitinated protein deubiquitination / double-strand break repair involved in meiotic recombination / homologous chromosome pairing at meiosis / gamete generation / neuronal stem cell population maintenance / brain morphogenesis / deubiquitinase activator activity / DNA repair complex / protein deubiquitination / mitotic intra-S DNA damage checkpoint signaling / skeletal system morphogenesis / skin development / condensed chromosome / seminiferous tubule development / homeostasis of number of cells / positive regulation of double-strand break repair via homologous recombination / single fertilization / embryonic organ development / regulation of DNA repair / interstrand cross-link repair / DNA polymerase binding / response to UV / positive regulation of epithelial cell proliferation / Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Membrane binding and targetting of GAG proteins / Regulation of TBK1, IKKε-mediated activation of IRF3, IRF7 upon TLR3 ligation / Endosomal Sorting Complex Required For Transport (ESCRT) / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Negative regulation of FLT3 / Constitutive Signaling by NOTCH1 HD Domain Mutants / Regulation of FZD by ubiquitination / TICAM1,TRAF6-dependent induction of TAK1 complex / TICAM1-dependent activation of IRF3/IRF7 / NOTCH2 Activation and Transmission of Signal to the Nucleus / APC/C:Cdc20 mediated degradation of Cyclin B / p75NTR recruits signalling complexes / Downregulation of ERBB4 signaling / NOTCH3 Activation and Transmission of Signal to the Nucleus / APC-Cdc20 mediated degradation of Nek2A / TRAF6-mediated induction of TAK1 complex within TLR4 complex / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / PINK1-PRKN Mediated Mitophagy / Pexophagy / Regulation of innate immune responses to cytosolic DNA / InlA-mediated entry of Listeria monocytogenes into host cells / VLDLR internalisation and degradation / Regulation of pyruvate metabolism / Downregulation of ERBB2:ERBB3 signaling / NF-kB is activated and signals survival / Regulation of PTEN localization / NRIF signals cell death from the nucleus / Regulation of BACH1 activity / Activated NOTCH1 Transmits Signal to the Nucleus / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Translesion synthesis by REV1 / MAP3K8 (TPL2)-dependent MAPK1/3 activation / Translesion synthesis by POLK / TICAM1, RIP1-mediated IKK complex recruitment / Downregulation of TGF-beta receptor signaling / EGFR downregulation / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / Josephin domain DUBs / Regulation of activated PAK-2p34 by proteasome mediated degradation / InlB-mediated entry of Listeria monocytogenes into host cell / IKK complex recruitment mediated by RIP1 / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / TNFR1-induced NF-kappa-B signaling pathway / Autodegradation of Cdh1 by Cdh1:APC/C / ubiquitin binding / positive regulation of protein ubiquitination / APC/C:Cdc20 mediated degradation of Securin / skeletal system development / Asymmetric localization of PCP proteins / TCF dependent signaling in response to WNT / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / Ubiquitin-dependent degradation of Cyclin D / Regulation of NF-kappa B signaling / AUF1 (hnRNP D0) binds and destabilizes mRNA 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) / synthetic construct (人工物) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.85 Å | |||||||||
データ登録者 | Rennie ML / Walden H | |||||||||
資金援助 | European Union, 英国, 2件
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引用 | ジャーナル: Sci Adv / 年: 2022 タイトル: Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site. 著者: Martin L Rennie / Connor Arkinson / Viduth K Chaugule / Helen Walden / 要旨: Repair of DNA damage is critical to genomic integrity and frequently disrupted in cancers. Ubiquitin-specific protease 1 (USP1), a nucleus-localized deubiquitinase, lies at the interface of multiple ...Repair of DNA damage is critical to genomic integrity and frequently disrupted in cancers. Ubiquitin-specific protease 1 (USP1), a nucleus-localized deubiquitinase, lies at the interface of multiple DNA repair pathways and is a promising drug target for certain cancers. Although multiple inhibitors of this enzyme, including one in phase 1 clinical trials, have been established, their binding mode is unknown. Here, we use cryo-electron microscopy to study an assembled enzyme-substrate-inhibitor complex of USP1 and the well-established inhibitor, ML323. Achieving 2.5-Å resolution, with and without ML323, we find an unusual binding mode in which the inhibitor disrupts part of the hydrophobic core of USP1. The consequent conformational changes in the secondary structure lead to subtle rearrangements in the active site that underlie the mechanism of inhibition. These structures provide a platform for structure-based drug design targeting USP1. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_14722.map.gz | 118.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-14722-v30.xml emd-14722.xml | 24.6 KB 24.6 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_14722_fsc.xml | 11.9 KB | 表示 | FSCデータファイル |
画像 | emd_14722.png | 179.7 KB | ||
マスクデータ | emd_14722_msk_1.map | 125 MB | マスクマップ | |
Filedesc metadata | emd-14722.cif.gz | 8.1 KB | ||
その他 | emd_14722_half_map_1.map.gz emd_14722_half_map_2.map.gz | 116 MB 116 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-14722 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14722 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_14722_validation.pdf.gz | 826.5 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_14722_full_validation.pdf.gz | 826.1 KB | 表示 | |
XML形式データ | emd_14722_validation.xml.gz | 19 KB | 表示 | |
CIF形式データ | emd_14722_validation.cif.gz | 24.2 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14722 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-14722 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_14722.map.gz / 形式: CCP4 / 大きさ: 125 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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注釈 | Globally sharpened map | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
ファイル | emd_14722_msk_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_14722_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_14722_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
-全体 : USP1(C90S)-UAF1 bound to FANCI and mono-ubiquitinated FANCD2 with...
全体 | 名称: USP1(C90S)-UAF1 bound to FANCI and mono-ubiquitinated FANCD2 with dsDNA and ML323 |
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要素 |
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-超分子 #1: USP1(C90S)-UAF1 bound to FANCI and mono-ubiquitinated FANCD2 with...
超分子 | 名称: USP1(C90S)-UAF1 bound to FANCI and mono-ubiquitinated FANCD2 with dsDNA and ML323 タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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-超分子 #2: Fanconi anemia group I protein, Fanconi anemia group D2 protein w...
超分子 | 名称: Fanconi anemia group I protein, Fanconi anemia group D2 protein with ubiquitin conjugated to K561, Ubiquitin carboxyl-terminal hydrolase 1, WD repeat-containing protein 48 タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: all |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
-分子 #1: Fanconi anemia group I protein
分子 | 名称: Fanconi anemia group I protein / タイプ: protein_or_peptide / ID: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) |
配列 | 文字列: MHHHHHHMDQ KILSLAAEKT ADKLQEFLQT LREGDLTNLL QNQAVKGKVA GALLRAIFKG SPCSEEAGTL RRRKIYTCCI QLVESGDLQK EIASEIIGLL MLEAHHFPGP LLVELANEFI SAVREGSLVN GKSLELLPII LTALATKKEN LAYGKGVLSG EECKKQLINT ...文字列: MHHHHHHMDQ KILSLAAEKT ADKLQEFLQT LREGDLTNLL QNQAVKGKVA GALLRAIFKG SPCSEEAGTL RRRKIYTCCI QLVESGDLQK EIASEIIGLL MLEAHHFPGP LLVELANEFI SAVREGSLVN GKSLELLPII LTALATKKEN LAYGKGVLSG EECKKQLINT LCSGRWDQQY VIQLTSMFKD VPLTAEEVEF VVEKALSMFS KMNLQEIPPL VYQLLVLSSK GSRKSVLEGI IAFFSALDKQ HNEEQSGDEL LDVVTVPSGE LRHVEGTIIL HIVFAIKLDY ELGRELVKHL KVGQQGDSNN NLSPFSIALL LSVTRIQRFQ DQVLDLLKTS VVKSFKDLQL LQGSKFLQNL VPHRSYVSTM ILEVVKNSVH SWDHVTQGLV ELGFILMDSY GPKKVLDGKT IETSPSLSRM PNQHACKLGA NILLETFKIH EMIRQEILEQ VLNRVVTRAS SPISHFLDLL SNIVMYAPLV LQSCSSKVTE AFDYLSFLPL QTVQRLLKAV QPLLKVSMSM RDCLILVLRK AMFANQLDAR KSAVAGFLLL LKNFKVLGSL SSSQCSQSLS VSQVHVDVHS HYNSVANETF CLEIMDSLRR CLSQQADVRL MLYEGFYDVL RRNSQLANSV MQTLLSQLKQ FYEPKPDLLP PLKLEACILT QGDKISLQEP LDYLLCCIQH CLAWYKNTVI PLQQGEEEEE EEEAFYEDLD DILESITNRM IKSELEDFEL DKSADFSQST SIGIKNNICA FLVMGVCEVL IEYNFSISSF SKNRFEDILS LFMCYKKLSD ILNEKAGKAK TKMANKTSDS LLSMKFVSSL LTALFRDSIQ SHQESLSVLR SSNEFMRYAV NVALQKVQQL KETGHVSGPD GQNPEKIFQN LCDITRVLLW RYTSIPTSVE ESGKKEKGKS ISLLCLEGLQ KIFSAVQQFY QPKIQQFLRA LDVTDKEGEE REDADVSVTQ RTAFQIRQFQ RSLLNLLSSQ EEDFNSKEAL LLVTVLTSLS KLLEPSSPQF VQMLSWTSKI CKENSREDAL FCKSLMNLLF SLHVSYKSPV ILLRDLSQDI HGHLGDIDQD VEVEKTNHFA IVNLRTAAPT VCLLVLSQAE KVLEEVDWLI TKLKGQVSQE TLSEEASSQA TLPNQPVEKA IIMQLGTLLT FFHELVQTAL PSGSCVDTLL KDLCKMYTTL TALVRYYLQV CQSSGGIPKN MEKLVKLSGS HLTPLCYSFI SYVQNKSKSL NYTGEKKEKP AAVATAMARV LRETKPIPNL IFAIEQYEKF LIHLSKKSKV NLMQHMKLST SRDFKIKGNI LDMVLREDGE DENEEGTASE HGGQNKEPAK KKRKK UniProtKB: Fanconi anemia group I protein |
-分子 #2: Fanconi anemia group D2 protein
分子 | 名称: Fanconi anemia group D2 protein / タイプ: protein_or_peptide / ID: 2 / 詳細: Ubiquitin conjugated to K561 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) |
配列 | 文字列: GPGSMVSKRR LSKSEDKESL TEDASKTRKQ PLSKKTKKSH IANEVEENDS IFVKLLKISG IILKTGESQN QLAVDQIAFQ KKLFQTLRRH PSYPKIIEEF VSGLESYIED EDSFRNCLLS CERLQDEEAS MGASYSKSLI KLLLGIDILQ PAIIKTLFEK LPEYFFENKN ...文字列: GPGSMVSKRR LSKSEDKESL TEDASKTRKQ PLSKKTKKSH IANEVEENDS IFVKLLKISG IILKTGESQN QLAVDQIAFQ KKLFQTLRRH PSYPKIIEEF VSGLESYIED EDSFRNCLLS CERLQDEEAS MGASYSKSLI KLLLGIDILQ PAIIKTLFEK LPEYFFENKN SDEINIPRLI VSQLKWLDRV VDGKDLTTKI MQLISIAPEN LQHDIITSLP EILGDSQHAD VGKELSDLLI ENTSLTVPIL DVLSSLRLDP NFLLKVRQLV MDKLSSIRLE DLPVIIKFIL HSVTAMDTLE VISELREKLD LQHCVLPSRL QASQVKLKSK GRASSSGNQE SSGQSCIILL FDVIKSAIRY EKTISEAWIK AIENTASVSE HKVFDLVMLF IIYSTNTQTK KYIDRVLRNK IRSGCIQEQL LQSTFSVHYL VLKDMCSSIL SLAQSLLHSL DQSIISFGSL LYKYAFKFFD TYCQQEVVGA LVTHICSGNE AEVDTALDVL LELVVLNPSA MMMNAVFVKG ILDYLDNISP QQIRKLFYVL STLAFSKQNE ASSHIQDDMH LVIRKQLSST VFKYKLIGII GAVTMAGIMA ADRSESPSLT QERANLSDEQ CTQVTSLLQL VHSCSEQSPQ ASALYYDEFA NLIQHEKLDP KALEWVGHTI CNDFQDAFVV DSCVVPEGDF PFPVKALYGL EEYDTQDGIA INLLPLLFSQ DFAKDGGPVT SQESGQKLVS PLCLAPYFRL LRLCVERQHN GNLEEIDGLL DCPIFLTDLE PGEKLESMSA KERSFMCSLI FLTLNWFREI VNAFCQETSP EMKGKVLTRL KHIVELQIIL EKYLAVTPDY VPPLGNFDVE TLDITPHTVT AISAKIRKKG KIERKQKTDG SKTSSSDTLS EEKNSECDPT PSHRGQLNKE FTGKEEKTSL LLHNSHAFFR ELDIEVFSIL HCGLVTKFIL DTEMHTEATE VVQLGPPELL FLLEDLSQKL ESMLTPPIAR RVPFLKNKGS RNIGFSHLQQ RSAQEIVHCV FQLLTPMCNH LENIHNYFQC LAAENHGVVD GPGVKVQEYH IMSSCYQRLL QIFHGLFAWS GFSQPENQNL LYSALHVLSS RLKQGEHSQP LEELLSQSVH YLQNFHQSIP SFQCALYLIR LLMVILEKST ASAQNKEKIA SLARQFLCRV WPSGDKEKSN ISNDQLHALL CIYLEHTESI LKAIEEIAGV GVPELINSPK DASSSTFPTL TRHTFVVFFR VMMAELEKTV KKIEPGTAAD SQQIHEEKLL YWNMAVRDFS ILINLIKVFD SHPVLHVCLK YGRLFVEAFL KQCMPLLDFS FRKHREDVLS LLETFQLDTR LLHHLCGHSK IHQDTRLTQH VPLLKKTLEL LVCRVKAMLT LNNCREAFWL GNLKNRDLQG EEIKSQNSQE STADESEDDM SSQASKSKAT EDGEEDEVSA GEKEQDSDES YDDSD UniProtKB: Fanconi anemia group D2 protein |
-分子 #3: Polyubiquitin-C
分子 | 名称: Polyubiquitin-C / タイプ: protein_or_peptide / ID: 3 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Escherichia coli BL21(DE3) (大腸菌) |
配列 | 文字列: GPGSMQIFVK TLTGKTITLE VEPSDTIENV KAKIQDKEGI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST LHLVLRLRGG |
-分子 #4: Ubiquitin carboxyl-terminal hydrolase 1
分子 | 名称: Ubiquitin carboxyl-terminal hydrolase 1 / タイプ: protein_or_peptide / ID: 4 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) |
配列 | 文字列: GMPGVIPSES NGLSRGSPSK KNRLSLKFFQ KKETKRALDF TDSQENEEKA SEYRASEIDQ VVPAAQSSPI NCEKRENLLP FVGLNNLGNT SYLNSILQVL YFCPGFKSGV KHLFNIISRK KEALKDEANQ KDKGNCKEDS LASYELICSL QSLIISVEQL QASFLLNPEK ...文字列: GMPGVIPSES NGLSRGSPSK KNRLSLKFFQ KKETKRALDF TDSQENEEKA SEYRASEIDQ VVPAAQSSPI NCEKRENLLP FVGLNNLGNT SYLNSILQVL YFCPGFKSGV KHLFNIISRK KEALKDEANQ KDKGNCKEDS LASYELICSL QSLIISVEQL QASFLLNPEK YTDELATQPR RLLNTLRELN PMYEGYLQHD AQEVLQCILG NIQETCQLLK KEEVKNVAEL PTKVEEIPHP KEEMNGINSI EMDSMRHSED FKEKLPKGNG KRKSDTEFGN MKKKVKLSKE HQSLEENQRQ TRSKRKATSD TLESPPKIIP KYISENESPR PSQKKSRVKI NWLKSATKQP SILSKFCSLG KITTNQGVKG QSKENECDPE EDLGKCESDN TTNGCGLESP GNTVTPVNVN EVKPINKGEE QIGFELVEKL FQGQLVLRTR CLECESLTER REDFQDISVP VQEDELSKVE ESSEISPEPK TEMKTLRWAI SQFASVERIV GEDKYFCENC HHYTEAERSL LFDKMPEVIT IHLKCFAASG LEFDCYGGGL SKINTPLLTP LKLSLEEWST KPTNDSYGLF AVVMHSGITI SSGHYTASVK VTDLNSLELD KGNFVVDQMC EIGKPEPLNE EEARGVVENY NDEEVSIRVG GNTQPSKVLN KKNVEAIGLL GGQKSKADYE LYNKASNPDK VASTAFAENR NSETSDTTGT HESDRNKESS DQTGINISGF ENKISYVVQS LKEYEGKWLL FDDSEVKVTE EKDFLNSLSP STSPTSTPYL LFYKKL UniProtKB: Ubiquitin carboxyl-terminal hydrolase 1 |
-分子 #5: WD repeat-containing protein 48
分子 | 名称: WD repeat-containing protein 48 / タイプ: protein_or_peptide / ID: 5 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
組換発現 | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) |
配列 | 文字列: MHHHHHHLEV LFQGPGSMAA HHRQNTAGRR KVQVSYVIRD EVEKYNRNGV NALQLDPALN RLFTAGRDSI IRIWSVNQHK QDPYIASMEH HTDWVNDIVL CCNGKTLISA SSDTTVKVWN AHKGFCMSTL RTHKDYVKAL AYAKDKELVA SAGLDRQIFL WDVNTLTALT ...文字列: MHHHHHHLEV LFQGPGSMAA HHRQNTAGRR KVQVSYVIRD EVEKYNRNGV NALQLDPALN RLFTAGRDSI IRIWSVNQHK QDPYIASMEH HTDWVNDIVL CCNGKTLISA SSDTTVKVWN AHKGFCMSTL RTHKDYVKAL AYAKDKELVA SAGLDRQIFL WDVNTLTALT ASNNTVTTSS LSGNKDSIYS LAMNQLGTII VSGSTEKVLR VWDPRTCAKL MKLKGHTDNV KALLLNRDGT QCLSGSSDGT IRLWSLGQQR CIATYRVHDE GVWALQVNDA FTHVYSGGRD RKIYCTDLRN PDIRVLICEE KAPVLKMELD RSADPPPAIW VATTKSTVNK WTLKGIHNFR ASGDYDNDCT NPITPLCTQP DQVIKGGASI IQCHILNDKR HILTKDTNNN VAYWDVLKAC KVEDLGKVDF EDEIKKRFKM VYVPNWFSVD LKTGMLTITL DESDCFAAWV SAKDAGFSSP DGSDPKLNLG GLLLQALLEY WPRTHVNPMD EEENEVNHVN GEQENRVQKG NGYFQVPPHT PVIFGEAGGR TLFRLLCRDS GGETESMLLN ETVPQWVIDI TVDKNMPKFN KIPFYLQPHA SSGAKTLKKD RLSASDMLQV RKVMEHVYEK IINLDNESQT TSSSNNEKPG EQEKEEDIAV LAEEKIELLC QDQVLDPNMD LRTVKHFIWK SGGDLTLHYR QKST UniProtKB: WD repeat-containing protein 48 |
-分子 #6: DNA (61-MER)
分子 | 名称: DNA (61-MER) / タイプ: dna / ID: 6 / 分類: DNA |
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由来(天然) | 生物種: synthetic construct (人工物) |
配列 | 文字列: TGATCAGAGG TCATTTGAAT TCATGGCTTC GAGCTTCATG TAGAGTCGAC GGTGCTGGGA T |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 8 |
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グリッド | モデル: UltrAuFoil R1.2/1.3 / メッシュ: 300 |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 288 K / 詳細: blotted for 3.0 secs before plunging. |
詳細 | 9.3 uM USP1-UAF1, 1.8 uM FANCI-FANCD2Ub, 2.2 uM dsDNA (61 base-pairs), 18 uM ML323 |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 40.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
+画像解析
-原子モデル構築 1
精密化 | 温度因子: 95.3 |
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得られたモデル | PDB-8a9k: |