登録情報 データベース : EMDB / ID : EMD-43482 ダウンロードとリンクタイトル Density map for gamma tubulin ring complex capped microtubule マップデータDensity map for gamma tubulin ring complex capped microtubule. 詳細 試料複合体 : Gamma tubulin ring complex bound to microtubule minus endタンパク質・ペプチド : Tubulin alpha-1B chainタンパク質・ペプチド : Tubulin beta-3 chainタンパク質・ペプチド : TUBGCP6 proteinタンパク質・ペプチド : Gamma-tubulin complex component 3タンパク質・ペプチド : Mitotic-spindle organizing protein 1タンパク質・ペプチド : Actin, cytoplasmic 1 アクチンタンパク質・ペプチド : Isoform 3 of Gamma-tubulin complex component 2タンパク質・ペプチド : Isoform 2 of Gamma-tubulin complex component 4タンパク質・ペプチド : Gamma-tubulin complex component 5タンパク質・ペプチド : Tubulin gamma-1 chain 残り6件を表示 表示を減らす 詳細 キーワード Microtubule nucleation complex bound to a microtubule / CELL CYCLE (細胞周期)機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
netrin receptor binding / microtubule nucleation by interphase microtubule organizing center / gamma-tubulin complex localization / positive regulation of norepinephrine uptake / equatorial microtubule organizing center / Post-chaperonin tubulin folding pathway / cellular response to cytochalasin B / dorsal root ganglion development / Carboxyterminal post-translational modifications of tubulin / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane ... netrin receptor binding / microtubule nucleation by interphase microtubule organizing center / gamma-tubulin complex localization / positive regulation of norepinephrine uptake / equatorial microtubule organizing center / Post-chaperonin tubulin folding pathway / cellular response to cytochalasin B / dorsal root ganglion development / Carboxyterminal post-translational modifications of tubulin / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / bBAF complex / mitotic spindle microtubule / Cilium Assembly / interphase microtubule organizing center / gamma-tubulin ring complex / npBAF complex / postsynaptic actin cytoskeleton organization / regulation of transepithelial transport / polar microtubule / brahma complex / nBAF complex / structural constituent of postsynaptic actin cytoskeleton / gamma-tubulin complex / morphogenesis of a polarized epithelium / meiotic spindle organization / cytoskeleton-dependent intracellular transport / GBAF complex / postsynaptic actin cytoskeleton / protein localization to adherens junction / Intraflagellar transport / Sealing of the nuclear envelope (NE) by ESCRT-III / Formation of annular gap junctions / regulation of G0 to G1 transition / dense body / Gap junction degradation / Tat protein binding / Cell-extracellular matrix interactions / Folding of actin by CCT/TriC / Gap junction assembly / microtubule nucleation / regulation of double-strand break repair / Formation of tubulin folding intermediates by CCT/TriC / regulation of nucleotide-excision repair / RSC-type complex / COPI-independent Golgi-to-ER retrograde traffic / apical protein localization / Prefoldin mediated transfer of substrate to CCT/TriC / non-motile cilium / adherens junction assembly / RHOF GTPase cycle / gamma-tubulin binding / Adherens junctions interactions / Kinesins / Assembly and cell surface presentation of NMDA receptors / 密着結合 / Sensory processing of sound by outer hair cells of the cochlea / SWI/SNF complex / Interaction between L1 and Ankyrins / Sensory processing of sound by inner hair cells of the cochlea / regulation of mitotic metaphase/anaphase transition / regulation of norepinephrine uptake / positive regulation of double-strand break repair / 微小管形成中心 / COPI-dependent Golgi-to-ER retrograde traffic / positive regulation of T cell differentiation / NuA4 histone acetyltransferase complex / regulation of synaptic vesicle endocytosis / apical junction complex / establishment or maintenance of cell polarity / maintenance of blood-brain barrier / 細胞結合 / positive regulation of stem cell population maintenance / positive regulation of double-strand break repair via homologous recombination / cortical cytoskeleton / 中心体マトリックス / nitric-oxide synthase binding / single fertilization / regulation of cyclin-dependent protein serine/threonine kinase activity / Recycling pathway of L1 / cell leading edge / regulation of G1/S transition of mitotic cell cycle / mitotic sister chromatid segregation / negative regulation of cell differentiation / 刷子縁 / RHOH GTPase cycle / kinesin binding / ヘルト萼状シナプス / EPH-ephrin mediated repulsion of cells / mitotic spindle assembly / RHO GTPases Activate WASPs and WAVEs / RHO GTPases activate IQGAPs / spindle assembly / positive regulation of myoblast differentiation / Hedgehog 'off' state / cytoplasmic microtubule / microtubule-based process / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / Activation of AMPK downstream of NMDARs / cytoplasmic microtubule organization 類似検索 - 分子機能 Mitotic-spindle organizing protein 1 / Mitotic-spindle organizing gamma-tubulin ring associated / Gamma-tubulin complex component 6, N-terminal / Gamma-tubulin complex component 6 N-terminus / チューブリン / Gamma tubulin complex component, C-terminal / Gamma-tubulin complex component, C-terminal domain superfamily / Gamma tubulin complex component C-terminal / Gamma-tubulin complex component protein / Gamma tubulin complex component protein, N-terminal ... Mitotic-spindle organizing protein 1 / Mitotic-spindle organizing gamma-tubulin ring associated / Gamma-tubulin complex component 6, N-terminal / Gamma-tubulin complex component 6 N-terminus / チューブリン / Gamma tubulin complex component, C-terminal / Gamma-tubulin complex component, C-terminal domain superfamily / Gamma tubulin complex component C-terminal / Gamma-tubulin complex component protein / Gamma tubulin complex component protein, N-terminal / Gamma tubulin complex component N-terminal / Actins signature 1. / Actin, conserved site / Actins signature 2. / Actin/actin-like conserved site / Actins and actin-related proteins signature. / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / アクチン / Actin family / アクチン / チューブリン / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / ATPase, nucleotide binding domain 類似検索 - ドメイン・相同性 TUBGCP6 protein / Tubulin gamma-1 chain / Actin, cytoplasmic 1 / Tubulin alpha-1B chain / Mitotic-spindle organizing protein 1 / Tubulin beta-3 chain / Gamma-tubulin complex component 3 / Gamma-tubulin complex component 5 / Gamma-tubulin complex component 2 / Gamma-tubulin complex component 4 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 7.7 Å 詳細 データ登録者Aher A / Urnavicius L / Kapoor TM 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) GM130234 米国
引用ジャーナル : Nat Struct Mol Biol / 年 : 2024タイトル : Structure of the γ-tubulin ring complex-capped microtubule.著者 : Amol Aher / Linas Urnavicius / Allen Xue / Kasahun Neselu / Tarun M Kapoor / 要旨 : Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules ... Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge components and establish lateral and longitudinal interactions between γ-tubulins and α-tubulins. Our structures suggest that γ-TuRC, an inefficient nucleation template owing to its splayed conformation, can transform into a compacted cap at the microtubule minus end and set the lattice architecture of cellular microtubules. 履歴 登録 2024年1月22日 - ヘッダ(付随情報) 公開 2024年3月27日 - マップ公開 2024年3月27日 - 更新 2024年4月24日 - 現状 2024年4月24日 処理サイト : RCSB / 状態 : 公開
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