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- PDB-3rw0: Crystal structure of the NavAb voltage-gated sodium channel (Met2... -

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Basic information

Entry
Database: PDB / ID: 3rw0
TitleCrystal structure of the NavAb voltage-gated sodium channel (Met221Cys, 2.95 A)
ComponentsIon transport proteinIon transporter
KeywordsMETAL TRANSPORT / tetrameric ion channel / voltage-gated sodium-selective ion channel / membrane
Function / homology
Function and homology information


monoatomic cation channel activity / identical protein binding / plasma membrane
Similarity search - Function
Voltage-gated potassium channels. Chain C / Voltage-gated cation channel calcium and sodium / Helix Hairpins - #70 / Voltage-dependent channel domain superfamily / Four Helix Bundle (Hemerythrin (Met), subunit A) / Ion transport domain / Ion transport protein / Helix Hairpins / Up-down Bundle / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / Ion transport protein
Similarity search - Component
Biological speciesArcobacter butzleri (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.95 Å
AuthorsPayandeh, J. / Scheuer, T. / Zheng, N. / Catterall, W.A.
CitationJournal: Nature / Year: 2011
Title: The crystal structure of a voltage-gated sodium channel.
Authors: Payandeh, J. / Scheuer, T. / Zheng, N. / Catterall, W.A.
History
DepositionMay 6, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 13, 2011Provider: repository / Type: Initial release
Revision 1.1Jul 27, 2011Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Ion transport protein
B: Ion transport protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)74,08912
Polymers67,3002
Non-polymers6,78910
Water362
1
A: Ion transport protein
B: Ion transport protein
hetero molecules

A: Ion transport protein
B: Ion transport protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)148,17824
Polymers134,5994
Non-polymers13,57920
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_455-x-1,-y,z1
Buried area21710 Å2
ΔGint-183 kcal/mol
Surface area44460 Å2
MethodPISA
Unit cell
Length a, b, c (Å)125.495, 125.815, 191.824
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number23
Space group name H-MI222

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Components

#1: Protein Ion transport protein / Ion transporter


Mass: 33649.789 Da / Num. of mol.: 2 / Mutation: M221C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arcobacter butzleri (bacteria) / Strain: RM4018 / Gene: Abu_1752 / Plasmid: pFastBac Dual / Production host: Trichoplusia ni (cabbage looper) / Strain (production host): Hi5 / References: UniProt: A8EVM5
#2: Chemical
ChemComp-PX4 / 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / Dimyristoylphosphatidylcholine


Mass: 678.940 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C36H73NO8P / Comment: DMPC, phospholipid*YM
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 5.63 Å3/Da / Density % sol: 78.13 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.75
Details: CHAPSO:DMPC bicelles, 2 M ammonium sulphate, 0.1 M Na-citrate pH 4.75, 28% glucose, nicotinic acid (sat.), VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 0.99994 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 2, 2010
RadiationMonochromator: DOUBLE CRYSTAL, SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.99994 Å / Relative weight: 1
ReflectionResolution: 2.95→50 Å / Num. all: 32343 / Num. obs: 31353 / % possible obs: 96.9 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 0 / Redundancy: 5.6 % / Rmerge(I) obs: 0.051 / Net I/σ(I): 31.79
Reflection shellResolution: 2.95→3.02 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.635 / Mean I/σ(I) obs: 1.53 / % possible all: 85.6

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Processing

Software
NameClassification
HKL-2000data collection
CNSrefinement
HKL-2000data reduction
SCALEPACKdata scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.95→50 Å / σ(F): 1 / Stereochemistry target values: Engh & Huber
Details: built using SAD data sets from Hg and SeMet crystals
RfactorNum. reflectionSelection details
Rfree0.2716 1554 random
Rwork0.2589 --
all0.2716 32343 -
obs0.2589 31350 -
Refinement stepCycle: LAST / Resolution: 2.95→50 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3652 0 127 2 3781
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_angle_d0.00946
X-RAY DIFFRACTIONc_angle_deg1.33729
LS refinement shellResolution: 2.95→2.98 Å
RfactorNum. reflection
Rfree0.3094 20
Rwork0.4326 -
obs-452

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