SASDBY6: Leishmania braziliensis Mitochondrial heat shock protein 70 (Lbmt...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDBY6

試料

Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70)

• Mitochondrial heat shock protein 70 (protein), LbmtHsp70, Leishmania braziliensis

• 生物種: Leishmania braziliensis (ブラジルリーシュマニア)
• 引用: ジャーナル: Arch Biochem Biophys / 年: 2017; タイトル: Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues.; 著者: Paulo R Dores-Silva / Letícia S Nishimura / Vanessa T R Kiraly / Júlio C Borges / ; 要旨: Heat shock protein 70 kDa (Hsp70) is a conserved molecular chaperone family involved in several functions related to protein homeostasis. In eukaryotes, Hsp70 homologues are found in all cell compartments. The mitochondrial Hsp70 isoform (mtHsp70) is involved in import of mitochondrial matrix proteins as well as their folding and maturation. Moreover, mtHsp70 has the propensity to self-aggregate, and it depends on the action of the co-chaperone Hsp70-escort protein 1 (Hep1) to be produced functional. Here, we analyze the solution structure and function of mtHsp70 of Leishmania braziliensis (LbmtHsp70). This recombinant protein was obtained folded, in the monomeric state and it has an elongated shape. We observed that LbmtHsp70 suffers thermal aggregation that depends on the protein concentration and is composed of domains with different thermal stabilities. LbmtHsp70 interacted with adenosine nucleotides with a thermodynamic signature different from those reported for human orthologues and interacted, driven by both enthalpy and entropy, with L. braziliensis Hep1 (LbHep1) with a nanomolar dissociation constant. Moreover, LbHep1 stimulated the LbmtHsp70 ATPase activity. Since little is known about mitochondrial Hsp70, particularly in protozoa, we believe that our data are of interest for understanding protozoan Hsp70 machinery.

登録者

• Júlio Borges (Instituto de Química de São Carlos, São Carlos - SP - Brasil)

モデル

• モデル #641: ; タイプ: dummy / ソフトウェア: DAMMIN / ダミー原子の半径: 3.25 A / カイ2乗値: 1.507 / P-value: 0.401000; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70); 試料濃度: 0.50-0.50
• バッファ: 名称: Tris-HCl 25 mM, 50 mM NacL, 5 mM KCl, 5 mM sodium phosphate, 2 mM B-mercaptoethanol; pH: 7.5

要素 #410

名称: LbmtHsp70 / タイプ: protein / 記述: Mitochondrial heat shock protein 70 / 分子量: 70.902 / 分子数: 1 / 由来: Leishmania braziliensis
配列:

MGSSHHHHHH SSGLVPRGSH MESQKVTGDV IGVDLGTTYS CVATMDGDKA RVLENSEGFR TTPSVVAFKG SEKLVGLAAK RQAITNPQST FYAVKRLIGR RFEDEHIQRD IKNVPYKIVR AGNGDAWVQD GNGKQYSPSQ VGAFVLEKMK ETAQNFLGHT VSNAVVTCPA YFNDAQRQAT KDAGTIAGLN VIRVVNEPTA AALAYGMDKT KDSLIAVYDL GGGTFDISVL EIAGGVFEVK ATNGDTHLGG EDFDLALSDY ILEEFRKTSG IDLSKERMAL QRVREAAEKA KCELSSAMET EVNLPFITAN ADGAQHIQMH ISRSKFEGIT TRLIERSIAP CKQCIKDAGV ELKEINDVVL VGGMTRMPKV VEEVKRFFQK EPFRGVNPDE AVALGAATLG GVLRGDVKGL VLLDVTPLSL GIETLGGVFT RMIPKNTTIP TKKSQTFSTA ADNQTQVGIK VFQGEREMAA DNQMMGQFDL VGIPPAPRGV PQVEVTFDID ANGICHVTAK DKATGKTQNI TITAHGGLSK EQIEQMVRDS EQHAEADRVK RELVEARNNA ETQLTTAERQ LGEWKYVSDA EKENVKTHVA ELRKAMENPN VAKDDLVAAT DKLQKAVMEC GRTEYQQAAA ANSGSSSNSG EQQQQQQQSS EKN

実験情報

• ビーム: 設備名称: Brazilian Synchrotron Light Laboratory SAXS1 Beamline; 地域: Campinas / 国: Brazil / 線源: X-ray synchrotron / 波長: 0.1488 Å / スペクトロメータ・検出器間距離: 1 mm
• 検出器: 名称: Pilatus 300K / タイプ: 20Hz

スキャン

タイトル: Leishmania braziliensis Mitochondrial heat shock p
測定日: 2012年6月22日 / 保管温度: 4 °C / セル温度: 25 °C / 照射時間: 60 sec. / フレーム数: 5 / 単位: 1/A /

	Min	Max
Q	0.0193	0.3508

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 265 /

	Min	Max
Q	0.01934	0.3508
P(R) point	1	265
R	0	140

結果

カーブのタイプ: single_conc

	実験値	Standard	Standard error	Porod
分子量	76 kDa	76 kDa	8	74 kDa
体積	-	-	-	118 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.02592	0.0001413	0.025262101	0.0025
慣性半径, Rg	3.779 nm	0.032	3.63 nm	0.2

	Min	Max	Error
D	-	14	1
Guinier point	1	14	-