SASDBY6: Leishmania braziliensis Mitochondrial heat shock protein 70 (Lbmt...

Basic information

• Entry: Database: SASBDB / ID: SASDBY6

Sample

Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70)

• Mitochondrial heat shock protein 70 (protein), LbmtHsp70, Leishmania braziliensis

• Biological species: Leishmania braziliensis (eukaryote)
• Citation: Journal: Arch Biochem Biophys / Year: 2017; Title: Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues.; Authors: Paulo R Dores-Silva / Letícia S Nishimura / Vanessa T R Kiraly / Júlio C Borges / ; Abstract: Heat shock protein 70 kDa (Hsp70) is a conserved molecular chaperone family involved in several functions related to protein homeostasis. In eukaryotes, Hsp70 homologues are found in all cell compartments. The mitochondrial Hsp70 isoform (mtHsp70) is involved in import of mitochondrial matrix proteins as well as their folding and maturation. Moreover, mtHsp70 has the propensity to self-aggregate, and it depends on the action of the co-chaperone Hsp70-escort protein 1 (Hep1) to be produced functional. Here, we analyze the solution structure and function of mtHsp70 of Leishmania braziliensis (LbmtHsp70). This recombinant protein was obtained folded, in the monomeric state and it has an elongated shape. We observed that LbmtHsp70 suffers thermal aggregation that depends on the protein concentration and is composed of domains with different thermal stabilities. LbmtHsp70 interacted with adenosine nucleotides with a thermodynamic signature different from those reported for human orthologues and interacted, driven by both enthalpy and entropy, with L. braziliensis Hep1 (LbHep1) with a nanomolar dissociation constant. Moreover, LbHep1 stimulated the LbmtHsp70 ATPase activity. Since little is known about mitochondrial Hsp70, particularly in protozoa, we believe that our data are of interest for understanding protozoan Hsp70 machinery.

Contact author

• Júlio Borges (Instituto de Química de São Carlos, São Carlos - SP - Brasil)

Models

• Model #641: ; Type: dummy / Software: DAMMIN / Radius of dummy atoms: 3.25 A / Chi-square value: 1.507 / P-value: 0.401000; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70); Specimen concentration: 0.50-0.50
• Buffer: Name: Tris-HCl 25 mM, 50 mM NacL, 5 mM KCl, 5 mM sodium phosphate, 2 mM B-mercaptoethanol; pH: 7.5

Entity #410

Name: LbmtHsp70 / Type: protein / Description: Mitochondrial heat shock protein 70 / Formula weight: 70.902 / Num. of mol.: 1 / Source: Leishmania braziliensis
Sequence:

MGSSHHHHHH SSGLVPRGSH MESQKVTGDV IGVDLGTTYS CVATMDGDKA RVLENSEGFR TTPSVVAFKG SEKLVGLAAK RQAITNPQST FYAVKRLIGR RFEDEHIQRD IKNVPYKIVR AGNGDAWVQD GNGKQYSPSQ VGAFVLEKMK ETAQNFLGHT VSNAVVTCPA YFNDAQRQAT KDAGTIAGLN VIRVVNEPTA AALAYGMDKT KDSLIAVYDL GGGTFDISVL EIAGGVFEVK ATNGDTHLGG EDFDLALSDY ILEEFRKTSG IDLSKERMAL QRVREAAEKA KCELSSAMET EVNLPFITAN ADGAQHIQMH ISRSKFEGIT TRLIERSIAP CKQCIKDAGV ELKEINDVVL VGGMTRMPKV VEEVKRFFQK EPFRGVNPDE AVALGAATLG GVLRGDVKGL VLLDVTPLSL GIETLGGVFT RMIPKNTTIP TKKSQTFSTA ADNQTQVGIK VFQGEREMAA DNQMMGQFDL VGIPPAPRGV PQVEVTFDID ANGICHVTAK DKATGKTQNI TITAHGGLSK EQIEQMVRDS EQHAEADRVK RELVEARNNA ETQLTTAERQ LGEWKYVSDA EKENVKTHVA ELRKAMENPN VAKDDLVAAT DKLQKAVMEC GRTEYQQAAA ANSGSSSNSG EQQQQQQQSS EKN

Experimental information

• Beam: Instrument name: Brazilian Synchrotron Light Laboratory SAXS1 Beamline; City: Campinas / 国: Brazil / Type of source: X-ray synchrotron / Wavelength: 0.1488 Å / Dist. spec. to detc.: 1 mm
• Detector: Name: Pilatus 300K / Type: 20Hz

Scan

Title: Leishmania braziliensis Mitochondrial heat shock p / Measurement date: Jun 22, 2012 / Storage temperature: 4 °C / Cell temperature: 25 °C / Exposure time: 60 sec. / Number of frames: 5 / Unit: 1/A /

	Min	Max
Q	0.0193	0.3508

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 265 /

	Min	Max
Q	0.01934	0.3508
P(R) point	1	265
R	0	140

Result

Type of curve: single_conc

	Experimental	Standard	Standard error	Porod
MW	76 kDa	76 kDa	8	74 kDa
Volume	-	-	-	118 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.02592	0.0001413	0.025262101	0.0025
Radius of gyration, Rg	3.779 nm	0.032	3.63 nm	0.2

	Min	Max	Error
D	-	14	1
Guinier point	1	14	-