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- SASDB95: Shigella outer membrane protein IcsA autotransporter -

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Basic information

Entry
Database: SASBDB / ID: SASDB95
SampleShigella outer membrane protein IcsA autotransporter
  • Outer membrane protein IcsA (53-758) (protein), IcsA, Shigella flexneri
Function / homology
Function and homology information


cell tip / cell outer membrane / periplasmic space / cell adhesion / cell surface / extracellular region
Similarity search - Function
Autochaperone Domain Type 1 / Autochaperone domain type 1 / Autotransporter-associated beta strand repeat / Passenger-associated-transport-repeat / Autotransporter beta-domain / Outer membrane autotransporter barrel / Autotransporter beta-domain / Autotransporter beta-domain profile. / Autotransporter beta-domain / Autotransporter beta-domain superfamily ...Autochaperone Domain Type 1 / Autochaperone domain type 1 / Autotransporter-associated beta strand repeat / Passenger-associated-transport-repeat / Autotransporter beta-domain / Outer membrane autotransporter barrel / Autotransporter beta-domain / Autotransporter beta-domain profile. / Autotransporter beta-domain / Autotransporter beta-domain superfamily / Autotransporter, pectate lyase C-like domain superfamily / Pectin lyase fold/virulence factor
Similarity search - Domain/homology
Outer membrane autotransporter IcsA
Similarity search - Component
Biological speciesShigella flexneri (bacteria)
CitationDate: 2016 Nov 23
Title: The Shigella Virulence Factor IcsA Relieves N-WASP Autoinhibition by Displacing the VCA Domain
Authors: Mauricio R / Jeffries C / Svergun D
Contact author
  • Cy M Jeffries
  • Janet Deane
  • Rui Mauricio

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #558
Type: dummy / Software: DAMMIN / Radius of dummy atoms: 3.40 A / Chi-square value: 1.858 / P-value: 0.841000
Search similar-shape structures of this assembly by Omokage search (details)
Model #559
Type: dummy / Software: GASBOR / Radius of dummy atoms: 1.90 A / Chi-square value: 1.86 / P-value: 0.841000
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Shigella outer membrane protein IcsA autotransporter
BufferName: Tris / Concentration: 50.00 mM / pH: 7.4 / Composition: 150 mM NaCl, 10 mM CaCl2, 3% v/v glycerol
Entity #385Name: IcsA / Type: protein / Description: Outer membrane protein IcsA (53-758) / Formula weight: 72.476 / Num. of mol.: 1 / Source: Shigella flexneri / References: UniProt: Q7BCK4
Sequence: TPLSGTQELH FSEDNYEKLL TPVDGLSPLG AGEDGMDAWY ITSSNPSHAS RTKLRINSDI MISAGHGGAG DNNDGNSCGG NGGDSITGSD LSIINQGMIL GGSGGSGADH NGDGGEAVTG DNLFIINGEI ISGGHGGDSY SDSDGGNGGD AVTGVNLPII NKGTISGGNG ...Sequence:
TPLSGTQELH FSEDNYEKLL TPVDGLSPLG AGEDGMDAWY ITSSNPSHAS RTKLRINSDI MISAGHGGAG DNNDGNSCGG NGGDSITGSD LSIINQGMIL GGSGGSGADH NGDGGEAVTG DNLFIINGEI ISGGHGGDSY SDSDGGNGGD AVTGVNLPII NKGTISGGNG GNNYGEGDGG NGGDAITGSS LSVINKGTFA GGNGGAAYGY GYDGYGGNAI TGDNLSVINN GAILGGNGGH WGDAINGSNM TIANSGYIIS GKEDDGTQNV AGNAIHITGG NNSLILHEGS VITGDVQVNN SSILKIINND YTGTTPTIEG DLCAGDCTTV SLSGNKFTVS GDVSFGENSS LNLAGISSLE ASGNMSFGNN VKVEAIINNW AQKDYKLLSA DKGITGFSVS NISIINPLLT TGAIDYTKSY ISDQNKLIYG LSWNDTDGDS HGEFNLKENA ELTVSTILAD NLSHHNINSW DGKSLTKSGE GTLILAEKNT YSGFTNINAG ILKMGTVEAM TRTAGVIVNK GATLNFSGMN QTVNTLLNSG TVLINNINAP FLPDPVIVTG NMTLEKNGHV ILNNSSSNVG QTYVQKGNWH GKGGILSLGA VLGNDNSKTD RLEIAGHASG ITYVAVTNEG GSGDKTLEGV QIISTDSSDK NAFIQKGRIV AGSYDYRLKQ GTVSGLNTNK WYLTSQMDNQ ESKQMSNQES TQMSSR

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: Outer membrane protein IcsA autotransporter / Measurement date: May 18, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 119 / Unit: 1/nm /
MinMax
Q0.076 3.0088
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 932 /
MinMax
Q0.0760164 2.67152
P(R) point1 932
R0 13.22
Result
Type of curve: other
Comments: The bead models displayed for IcsA are individual/representative low-resolution structural examples calculated using DAMMIN or GASBOR. A cohort of individual GASBOR and DAMMIN models are ...Comments: The bead models displayed for IcsA are individual/representative low-resolution structural examples calculated using DAMMIN or GASBOR. A cohort of individual GASBOR and DAMMIN models are supplied in the full entry zip archive and include the average DAMMIN-based spatial representation of IcsA in solution (damfilt.pdb; NSD = 0.7). Individual reduced SEC-SAXS data frames/blocks and Rg/I(0) analysis through the IcsA elution peak are included in the full entry zip archive.
ExperimentalPorod
MW65 kDa65 kDa
Volume-103 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.7582 0.0022 0.75 0.0022
Radius of gyration, Rg3.76 nm0.02 3.65 nm0.02

MinMax
D-13.22
Guinier point6 102

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