+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDB95 |
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Sample | Shigella outer membrane protein IcsA autotransporter
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Function / homology | Function and homology information cell tip / cell outer membrane / periplasmic space / cell adhesion / cell surface / extracellular region Similarity search - Function |
Biological species | Shigella flexneri (bacteria) |
Citation | Date: 2016 Nov 23 Title: The Shigella Virulence Factor IcsA Relieves N-WASP Autoinhibition by Displacing the VCA Domain Authors: Mauricio R / Jeffries C / Svergun D |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #558 | Type: dummy / Software: DAMMIN / Radius of dummy atoms: 3.40 A / Chi-square value: 1.858 / P-value: 0.841000 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #559 | Type: dummy / Software: GASBOR / Radius of dummy atoms: 1.90 A / Chi-square value: 1.86 / P-value: 0.841000 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Shigella outer membrane protein IcsA autotransporter |
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Buffer | Name: Tris / Concentration: 50.00 mM / pH: 7.4 / Composition: 150 mM NaCl, 10 mM CaCl2, 3% v/v glycerol |
Entity #385 | Name: IcsA / Type: protein / Description: Outer membrane protein IcsA (53-758) / Formula weight: 72.476 / Num. of mol.: 1 / Source: Shigella flexneri / References: UniProt: Q7BCK4 Sequence: TPLSGTQELH FSEDNYEKLL TPVDGLSPLG AGEDGMDAWY ITSSNPSHAS RTKLRINSDI MISAGHGGAG DNNDGNSCGG NGGDSITGSD LSIINQGMIL GGSGGSGADH NGDGGEAVTG DNLFIINGEI ISGGHGGDSY SDSDGGNGGD AVTGVNLPII NKGTISGGNG ...Sequence: TPLSGTQELH FSEDNYEKLL TPVDGLSPLG AGEDGMDAWY ITSSNPSHAS RTKLRINSDI MISAGHGGAG DNNDGNSCGG NGGDSITGSD LSIINQGMIL GGSGGSGADH NGDGGEAVTG DNLFIINGEI ISGGHGGDSY SDSDGGNGGD AVTGVNLPII NKGTISGGNG GNNYGEGDGG NGGDAITGSS LSVINKGTFA GGNGGAAYGY GYDGYGGNAI TGDNLSVINN GAILGGNGGH WGDAINGSNM TIANSGYIIS GKEDDGTQNV AGNAIHITGG NNSLILHEGS VITGDVQVNN SSILKIINND YTGTTPTIEG DLCAGDCTTV SLSGNKFTVS GDVSFGENSS LNLAGISSLE ASGNMSFGNN VKVEAIINNW AQKDYKLLSA DKGITGFSVS NISIINPLLT TGAIDYTKSY ISDQNKLIYG LSWNDTDGDS HGEFNLKENA ELTVSTILAD NLSHHNINSW DGKSLTKSGE GTLILAEKNT YSGFTNINAG ILKMGTVEAM TRTAGVIVNK GATLNFSGMN QTVNTLLNSG TVLINNINAP FLPDPVIVTG NMTLEKNGHV ILNNSSSNVG QTYVQKGNWH GKGGILSLGA VLGNDNSKTD RLEIAGHASG ITYVAVTNEG GSGDKTLEGV QIISTDSSDK NAFIQKGRIV AGSYDYRLKQ GTVSGLNTNK WYLTSQMDNQ ESKQMSNQES TQMSSR |
-Experimental information
Beam | Instrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||||||
Scan | Title: Outer membrane protein IcsA autotransporter / Measurement date: May 18, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 119 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 932 /
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Result | Type of curve: other Comments: The bead models displayed for IcsA are individual/representative low-resolution structural examples calculated using DAMMIN or GASBOR. A cohort of individual GASBOR and DAMMIN models are ...Comments: The bead models displayed for IcsA are individual/representative low-resolution structural examples calculated using DAMMIN or GASBOR. A cohort of individual GASBOR and DAMMIN models are supplied in the full entry zip archive and include the average DAMMIN-based spatial representation of IcsA in solution (damfilt.pdb; NSD = 0.7). Individual reduced SEC-SAXS data frames/blocks and Rg/I(0) analysis through the IcsA elution peak are included in the full entry zip archive.
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