+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDA99 |
---|---|
Sample | Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor
|
Function / homology | Function and homology information interleukin-2 receptor binding / positive regulation of plasma cell differentiation / leukocyte activation involved in immune response / negative regulation of B cell apoptotic process / positive regulation of immunoglobulin production / positive regulation of activated T cell proliferation / positive regulation of interleukin-17 production / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / cytokine activity ...interleukin-2 receptor binding / positive regulation of plasma cell differentiation / leukocyte activation involved in immune response / negative regulation of B cell apoptotic process / positive regulation of immunoglobulin production / positive regulation of activated T cell proliferation / positive regulation of interleukin-17 production / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / cytokine activity / growth factor activity / adaptive immune response / extracellular space Similarity search - Function |
Biological species | Orf virus Ovis aries (sheep) |
Citation | Date: 2016 Nov 7 Title: Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF Authors: Felix J / Kandiah E / De Munck S / Bloch Y / van Zundert G / Pauwels K / Dansercoer A / Novanska K / Read R / Bonvin A / Vergauwen B / Verstraete K / Gutsche I |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Data source
SASBDB page | SASDA99 |
---|
-Related structure data
Related structure data | C: citing same article (ref.) |
---|---|
Similar structure data |
-External links
Related items in Molecule of the Month |
---|
-Models
Model #550 | Type: mix / Software: FoxS / Radius of dummy atoms: 1.90 A / Chi-square value: 35.9502544171 Search similar-shape structures of this assembly by Omokage search (details) |
---|
-Sample
Sample | Name: Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor Purity method: SEC-SAXS / Specimen concentration: 8.8 mg/ml / Entity id: 209 / 212 |
---|---|
Buffer | Name: HEPES / Concentration: 20.00 mM / pH: 7.4 / Composition: 150 mM NaCl |
Entity #209 | Type: protein / Description: GM-CSF/IL-2 inhibition factor / Formula weight: 29.924 / Num. of mol.: 4 / Source: Orf virus / References: UniProt: Q9J5U5 Sequence: MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID ...Sequence: MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID YPHTGMEFLQ HVLRPNRRFA PAKLHMDLEV DHRCVSAVHV KAFLQDACSA RKARTPLYFA GHGCNHPDRR PKNPVPRPQH VSSPISRKCS MQTAR |
Entity #212 | Type: protein / Description: Interleukin-2 / Formula weight: 15.56 / Num. of mol.: 1 / Source: Ovis aries / References: UniProt: P19114 Sequence: APTSSSTGNT MKEVKSLLLD LQLLLEKVKN PENLKLSRMH TFNFYMPKVN ATELKHLKCL LEELKLLEEV LDLAPSKNLN TREIKDSMDN IKRIVLELQG SETRFTCEYD DATVKAVEFL NKWITFCQSI YSTMT |
-Experimental information
Beam | Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.82 mm | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: AVIEX / Type: CCD | ||||||||||||||||||||||||||||||||||||
Scan | Title: complex between ovine IL-2 and GM-CSF/IL-2 inhibit / Measurement date: Sep 10, 2014 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 12 / Unit: 1/nm /
| ||||||||||||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 472 /
| ||||||||||||||||||||||||||||||||||||
Result | Type of curve: other Comments: Modeling of SAXS data: A model of the GIF:IL-2 complex, based on the fit in a low-resolution (24 Å) map obtained by Negative-Stain Electron Microscopy, was used as an input for the ...Comments: Modeling of SAXS data: A model of the GIF:IL-2 complex, based on the fit in a low-resolution (24 Å) map obtained by Negative-Stain Electron Microscopy, was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked glycosylation. During each Allosmod-FoXS run, data up to a scattering angle of 0.5 Å-1 was used. Fits to the experimental SAXS data were calculated using the FoXS software. Note that the exact orientation of IL-2 is uncertain due to the low resolution of the EM map.
|