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- SASDBB4: Plakin domain fragment of Human plectin (C-terminal region spectr... -

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Basic information

Entry
Database: SASBDB / ID: SASDBB4
SamplePlakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9)
  • Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 (protein), Plectin, Homo sapiens
Function / homologyIsoform 2 of Plectin
Function and homology information
Biological speciesHomo sapiens (human)
CitationJournal: J Biol Chem / Year: 2016
Title: The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.
Authors: Esther Ortega / José A Manso / Rubén M Buey / Ana M Carballido / Arturo Carabias / Arnoud Sonnenberg / José M de Pereda /
Abstract: Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins ...Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins contain a plakin domain formed by up to nine spectrin repeats (SR1-SR9) and an SH3 domain. The plakin domains of plectin and other plakins harbor binding sites for junctional proteins. We have combined x-ray crystallography with small angle x-ray scattering (SAXS) to elucidate the structure of the plakin domain of plectin, extending our previous analysis of the SR1 to SR5 region. Two crystal structures of the SR5-SR6 region allowed us to characterize its uniquely wide inter-repeat conformational variability. We also report the crystal structures of the SR7-SR8 region, refined to 1.8 Å, and the SR7-SR9 at lower resolution. The SR7-SR9 region, which is conserved in all other plakin domains, forms a rigid segment stabilized by uniquely extensive inter-repeat contacts mediated by unusually long helices in SR8 and SR9. Using SAXS we show that in solution the SR3-SR6 and SR7-SR9 regions are rod-like segments and that SR3-SR9 of plectin has an extended shape with a small central kink. Other plakins, such as bullous pemphigoid antigen 1 and microtubule and actin cross-linking factor 1, are likely to have similar extended plakin domains. In contrast, desmoplakin has a two-segment structure with a central flexible hinge. The continuous versus segmented structures of the plakin domains of plectin and desmoplakin give insight into how different plakins might respond to tension and transmit mechanical signals.
Contact author
  • Jose M de Pereda (USAL, La Universidad de Salamanca, Salamanca, Spain)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #495
Type: dummy / Software: DAMMIF / Radius of dummy atoms: 3.60 A / Chi-square value: 0.955 / P-value: 0.667500
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Plakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9)
Specimen concentration: 1.50-12.30
BufferName: sodium phosphate / Concentration: 20.00 mM / pH: 7.5 / Composition: 150 mM NaCl, 5% glycerol, 3 mM DTT
Entity #320Name: Plectin / Type: protein
Description: Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9
Formula weight: 42.825 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q15149-2
Sequence: GSHMQEESRC QRCISELKDI RLQLEACETR TVHRLRLPLD KEPARECAQR IAEQQKAQAE VEGLGKGVAR LSAEAEKVLA LPEPSPAAPT LRSELELTLG KLEQVRSLSA IYLEKLKTIS LVIRGTQGAE EVLRAHEEQL KEAQAVPATL PELEATKASL KKLRAQAEAQ ...Sequence:
GSHMQEESRC QRCISELKDI RLQLEACETR TVHRLRLPLD KEPARECAQR IAEQQKAQAE VEGLGKGVAR LSAEAEKVLA LPEPSPAAPT LRSELELTLG KLEQVRSLSA IYLEKLKTIS LVIRGTQGAE EVLRAHEEQL KEAQAVPATL PELEATKASL KKLRAQAEAQ QPTFDALRDE LRGAQEVGER LQQRHGERDV EVERWRERVA QLLERWQAVL AQTDVRQREL EQLGRQLRYY RESADPLGAW LQDARRRQEQ IQAMPLADSQ AVREQLRQEQ ALLEEIERHG EKVEECQRFA KQYINAIKDY ELQLVTYKAQ LEPVASPAKK PKVQSGSESV IQEYVDLRTH YSELTTLTSQ YIKFISETLR RME

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: Plakin domain fragment of Human plectin / Measurement date: Nov 26, 2013 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0539 4.4504
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1126 /
MinMax
Q0.0538541 3.01385
P(R) point1 1126
R0 17
Result
D max: 17 / Type of curve: extrapolated /
ExperimentalPorod
MW38 kDa35.9 kDa
Volume-57.4 nm3

P(R)Guinier
Forward scattering, I0878 873
Radius of gyration, Rg4.41 nm4.23 nm

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