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Yorodumi- SASDBQ3: Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid ... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDBQ3 |
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Sample | Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164)
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Function / homology | Function and homology information |
Biological species | Middle East respiratory syndrome coronavirus |
Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2016 Title: Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering. Authors: Nicolas Papageorgiou / Julie Lichière / Amal Baklouti / François Ferron / Marion Sévajol / Bruno Canard / Bruno Coutard / Abstract: The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N-terminal part is composed of an intrinsically disordered region (IDR) followed by a ...The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N-terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N-terminal domain (NTD). In this study, the structure determination of the N-terminal region of the MERS-CoV N protein via X-ray diffraction measurements is reported at a resolution of 2.4 Å. Since the first 30 amino acids were not resolved by X-ray diffraction, the structural study was completed by a SAXS experiment to propose a structural model including the IDR. This model presents the N-terminal region of the MERS-CoV as a monomer that displays structural features in common with other coronavirus NTDs. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #453 | Type: dummy / Software: DAMMIF (r4556) / Radius of dummy atoms: 1.70 A / Chi-square value: 0.408 / P-value: 0.015000 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #454 | Type: mix / Software: EOM / Radius of dummy atoms: 1.90 A / Chi-square value: 0.509 Search similar-shape structures of this assembly by Omokage search (details) |
Model #455 | Type: mix / Software: EOM / Radius of dummy atoms: 1.90 A / Chi-square value: 0.509 Search similar-shape structures of this assembly by Omokage search (details) |
Model #456 | Type: mix / Software: EOM / Radius of dummy atoms: 1.90 A / Chi-square value: 0.509 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) Specimen concentration: 0.21-3.06 |
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Buffer | Name: HEPES / Concentration: 10.00 mM / pH: 7.5 / Composition: 300 mM NaCl |
Entity #303 | Name: MERS N-Protein / Type: protein Description: Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) Formula weight: 17.788 / Num. of mol.: 1 / Source: Middle East respiratory syndrome coronavirus / References: UniProt: T2B9R0 Sequence: MASPAAPRAV SFADNNDITN TNLSRGRGRN PKPRAAPNNT VSWYTGLTQH GKVPLTFPPG QGVPLNANST PAQNAGYWRR QDRKINTGNG IKQLAPRWYF YYTGTGPEAA LPFRAVKDGI VWVHEDGATD APSTFGTRNP NNDSAIVTQF APGTKLPKNF HIEG |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.93 Å / Dist. spec. to detc.: 2.43 mm | |||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M | |||||||||||||||||||||||||||
Scan | Title: MERS N-Protein / Measurement date: Oct 5, 2015 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 974 /
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Result | Type of curve: merged /
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