SASDAU8: Immunosuppressive virulence protein YopM (Yersinia outer protein ...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAU8

試料

Immunosuppressive virulence protein YopM

• Yersinia outer protein M (34-481) (protein), YopM, Yersinia enterocolitica

• 生物種: Yersinia enterocolitica (腸炎エルシニア)
• 引用: ジャーナル: PLoS Pathog / 年: 2016; タイトル: Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.; 著者: Laura Berneking / Marie Schnapp / Andreas Rumm / Claudia Trasak / Klaus Ruckdeschel / Malik Alawi / Adam Grundhoff / Alexey G Kikhney / Friedrich Koch-Nolte / Friedrich Buck / Markus Perbandt / Christian Betzel / Dmitri I Svergun / Moritz Hentschke / Martin Aepfelbacher / ; 要旨: Yersinia outer protein M (YopM) is a crucial immunosuppressive effector of the plaque agent Yersinia pestis and other pathogenic Yersinia species. YopM enters the nucleus of host cells but neither the mechanisms governing its nucleocytoplasmic shuttling nor its intranuclear activities are known. Here we identify the DEAD-box helicase 3 (DDX3) as a novel interaction partner of Y. enterocolitica YopM and present the three-dimensional structure of a YopM:DDX3 complex. Knockdown of DDX3 or inhibition of the exportin chromosomal maintenance 1 (CRM1) increased the nuclear level of YopM suggesting that YopM exploits DDX3 to exit the nucleus via the CRM1 export pathway. Increased nuclear YopM levels caused enhanced phosphorylation of Ribosomal S6 Kinase 1 (RSK1) in the nucleus. In Y. enterocolitica infected primary human macrophages YopM increased the level of Interleukin-10 (IL-10) mRNA and this effect required interaction of YopM with RSK and was enhanced by blocking YopM's nuclear export. We propose that the DDX3/CRM1 mediated nucleocytoplasmic shuttling of YopM determines the extent of phosphorylation of RSK in the nucleus to control transcription of immunosuppressive cytokines.

登録者

• Al Kikhney (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #344: ; タイプ: atomic / ソフトウェア: SASREF (7) / 対称性: P2 / カイ2乗値: 2.317; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #347: ; タイプ: dummy / ソフトウェア: GASBOR (2.3i) / ダミー原子の半径: 1.90 A / 対称性: P2 / カイ2乗値: 2.72; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Immunosuppressive virulence protein YopM / 試料濃度: 0.65-10.44
• バッファ: 名称: HEPES / 濃度: 50.00 mM / pH: 7.5 / 組成: 150 mM NaCl

要素 #207

名称: YopM / タイプ: protein / 記述: Yersinia outer protein M (34-481) / 分子量: 50.321 / 分子数: 2 / 由来: Yersinia enterocolitica
配列:

KSKTEYYNAW SEWERNAPPG NGEQREMAVS RLRDCLDRQA HELELNNLGL SSLPELPPHL ERLVASCNSL TELPELPQSL KSLEVYENNL KALPDLPPLL VDLRVFNNQL EELPELQNLP FLTEIYANNN SLKTLPDLPP SLVDLNVREN YLTALPELPQ SLIFLDISDN ILSGLSELPP NLSCLDASRN GIRSLCDLPP SLVYLDVRDN QLIELPALPS GLERLIASFN HLAELPELPP NLYYLDASRN EISSLCDLPP SLVDLNVRKN QLIELPALPP DLERLIASFN HLAELPELPP NLSYLDASRN EISSLCDLPP SLVDLNVRKN QLIELPALPP DLERLIASFN HLAELPELPP NLSYLDASRN EISSLCDLPP SLVELDVRDN QLIELPALPP HLERLIASLN HLAEVPELPQ NLKQLHVEHN ALREFPDIPE SVEDLRMD

実験情報

• ビーム: 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.12 Å / スペクトロメータ・検出器間距離: 3.1 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: YopM is dimer in solution / 測定日: 2013年11月13日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 0.05 sec. / フレーム数: 20 / 単位: 1/nm /

	Min	Max
Q	0.0817	4.4911

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 779 /

	Min	Max
Q	0.10545	2.15471
P(R) point	1	779
R	0	11.5

結果

カーブのタイプ: extrapolated / コメント: YopM_34-481 is a dimer in solution

	実験値	Experimental error	Porod	Porod error
分子量	108 kDa	11	96 kDa	10
体積	-	-	153.8 nm3	-

	Guinier	P(R)	Guinier error
前方散乱 I0	16939.3	-	-
慣性半径, Rg	3.88 nm	3.855 nm	0.11

	Min	Max	Error
D	-	11.5	1.2
Guinier point	1	85	-