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- SASDAC8: SDS hydrolase SdsA1 (Pseudomonas aeruginosa SDS hydrolase SdsA1) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDAC8
SampleSDS hydrolase SdsA1
  • Pseudomonas aeruginosa SDS hydrolase SdsA1 (protein), Pseudomonas aeruginosa
Function / homology
Function and homology information


linear primary-alkylsulfatase / linear primary-alkylsulfatase activity / dodecyl sulfate metabolic process / outer membrane-bounded periplasmic space / protein dimerization activity / identical protein binding / metal ion binding
Similarity search - Function
Alkyl/aryl-sulfatase Bds1/ SdsA1 , MBL-fold / Alkyl sulfatase dimerisation domain / Alkyl sulfatase, C-terminal / Alkyl/aryl-sulfatase, dimerisation domain superfamily / Alkyl sulfatase dimerisation / Alkyl sulfatase C-terminal / SCP2 sterol-binding domain superfamily / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase / Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
Similarity search - Domain/homology
Linear primary-alkylsulfatase
Similarity search - Component
Biological speciesPseudomonas aeruginosa (bacteria)
CitationDate: 2015 Oct 1
Title: SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques
Authors: De la Mora E / Flores-Hernández E / Jakoncic J / Stojanoff V / Siliqi D / Sánchez-Puig N
Contact author
  • Eugenio De la Mora
  • Dritan Siliqi

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #320
Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 7.697
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: SDS hydrolase SdsA1 / Specimen concentration: 12.2 mg/ml
BufferName: 50 mM HEPES / Concentration: 50.00 mM / pH: 7
Entity #185Type: protein / Description: Pseudomonas aeruginosa SDS hydrolase SdsA1 / Formula weight: 72.591 / Num. of mol.: 2 / Source: Pseudomonas aeruginosa / References: UniProt: Q9I5I9
Sequence: MSRLLALLAL APLLAGAAET TAPKPPSAFT VEAQRRVEAE LPFADRADFE RADRGLIRRP ERLLIRNPDG SVAWQLGGYD FLLDGKPRDS INPSLQRQAL LNLKYGLFEV AEGIYQVRGF DLANITFIRG DSGWIVVDTL TTPATARAAY ELVSRELGER PIRTVIYSHA ...Sequence:
MSRLLALLAL APLLAGAAET TAPKPPSAFT VEAQRRVEAE LPFADRADFE RADRGLIRRP ERLLIRNPDG SVAWQLGGYD FLLDGKPRDS INPSLQRQAL LNLKYGLFEV AEGIYQVRGF DLANITFIRG DSGWIVVDTL TTPATARAAY ELVSRELGER PIRTVIYSHA HADHFGGVRG LVEPQQVASG AVQIIAPAGF MEAAIKENVL AGNAMMRRAT YQYGTQLPKG PQGQVDMAIG KGLARGPLSL LAPTRLIEGE GEDLVLDGVP FTFQNTPGTE SPAEMNIWLP RQKALLMAEN VVGTLHNLYT LRGAEVRDAL GWSKYINQAL HRFGRQAEVM FAVHNWPRWG NAEIVEVLEK QRDLYGYLHD QTLHLANQGV TIGQVHNRLR LPPSLDQEWY DRGYHGSVSH NARAVLNRYL GYYDGNPATL DPLSPEDSAG RYVEYMGGAE RLLEQARASY ARGEYRWVVE VVNRLVFAEP DNRAARELQA DALEQLGYQA ENAGWRNSYL SAAYELRHGV PRDQPTMKAG SADALAAMDT GLLFDYLGVR LDAGAAEGKA LSINLRLPDI GENYLLELKN SHLNNLRGVQ SEDAGQTVSI DRADLNRLLL KEVSAVRLVF EGKLKSSGNP LLLGQLFGML GDFDFWFDIV TPAAKSEG

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Experimental information

BeamInstrument name: National Synchrotron Light Source (NSLS) X9A
City: Brookhaven, NY / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.091 Å / Dist. spec. to detc.: 3.4 mm
DetectorName: Pilatus 300K / Type: 20Hz
Scan
Title: SDS hydrolase SdsA1 / Measurement date: Jul 22, 2013 / Storage temperature: 20 °C / Exposure time: 30 sec. / Number of frames: 6 / Unit: 1/nm /
MinMax
Q0.08 19.6
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 71 /
MinMax
Q0.18 1.65
P(R) point1 71
R0 16.91
Result
D max: 16.9 / Type of curve: single_conc /
ExperimentalPorod
MW140 kDa153 kDa
Volume-261 nm3

P(R)GuinierGuinier error
Forward scattering, I01607 1590 -
Radius of gyration, Rg3.66 nm3.6 nm0.89

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