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- SASDF94: Insulin glulisine (Apidra), oligomeric composition -

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Basic information

Entry
Database: SASBDB / ID: SASDF94
SampleInsulin glulisine (Apidra), oligomeric composition
  • Insulin glulisine (protein)
CitationDate: 2019 Jul
Title: The quaternary structure of insulin glargine and glulisine under formulation conditions
Authors: Nagel N / Graewert M / Gao M / Heyse W / Jeffries C / Svergun D
Contact author
  • Melissa Graewert (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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Models

Model #2919
Type: atomic / Comment: monomeric unit / Chi-square value: 1.03 / P-value: 0.574548
Search similar-shape structures of this assembly by Omokage search (details)
Model #2920
Type: atomic / Comment: dimeric unit / Chi-square value: 1.03 / P-value: 0.574548
Search similar-shape structures of this assembly by Omokage search (details)
Model #2921
Type: atomic / Comment: hexameric unit / Chi-square value: 1.03 / P-value: 0.574548
Search similar-shape structures of this assembly by Omokage search (details)
Model #2922
Type: atomic / Chi-square value: 1.03 / P-value: 0.574548
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Insulin glulisine (Apidra), oligomeric composition / Specimen concentration: 3.49 mg/ml
BufferName: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20)
pH: 7.3
Entity #1567Type: protein / Description: Insulin glulisine / Formula weight: 5.811 / Num. of mol.: 6
Sequence:
GIVEQCCTSI CSLYQLENYC NFVKQHLCGS HLVEALYLVC GERGFFYTPE T

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.1241 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: Insulin glulisine (Apidra), oligomeric composition / Measurement date: Apr 20, 2017 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/A /
MinMax
Q0.0095 0.5034
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1225 /
MinMax
Q0.0097679 0.346554
P(R) point1 1225
R0 76.22
Result
Type of curve: single_conc
Comments: Here, Apidra measured at 3.49 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions in placebo have been deposited in addition. Mixture analysis of the scattering ...Comments: Here, Apidra measured at 3.49 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions in placebo have been deposited in addition. Mixture analysis of the scattering profiles was performed with the program Oligomer. The SAXS data show that Apidra® primarily consists of hexamers, which dissociate into monomers upon dilution. The presence of dimers was not required to fit the experimental data, and their volume fraction was always essentially zero. A small volume fraction is, however, also made up by larger dodecameric species. The distribution between monomer, dimer, hexamer and dodecamer is documented in the logfile. Calculation of the form factors is based on an internal model (Apidra.pdb) for hexamers and 3W80.pdb for dodecamers.
ExperimentalStandardStandard error
MW34 kDa34 kDa5

P(R)GuinierGuinier error
Forward scattering, I062680 62549.3 147.88
Radius of gyration, Rg2.33 nm2.31 nm0.2

MinMax
D-7.62
Guinier point2 171

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