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- SASDED9: Relaxase domain of TraI (Relaxase (Tra_2) domain of TraI, Tra_2) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDED9
SampleRelaxase domain of TraI
  • Relaxase (Tra_2) domain of TraI (protein), Tra_2, Neisseria gonorrhoeae
Function / homologyUncharacterised domain, helicase/relaxase, putative / Putative helicase / Putative conjugal transfer nickase/helicase TraI, C-terminal / Putative conjugal transfer nickase/helicase TraI C-term / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / TraI
Function and homology information
Biological speciesNeisseria gonorrhoeae (bacteria)
CitationDate: 2019 Jul 5
Title: DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island
Authors: Heilers J / Reiners J / Heller E / Golzer A / Smits S
Contact author
  • Jens Reiners (Heinrich Heine University Düsseldorf, Düsseldorf, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2744
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 3.12 / P-value: 0.000001
Search similar-shape structures of this assembly by Omokage search (details)
Model #2745
Type: atomic / Symmetry: P1 / Chi-square value: 91.005
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Relaxase domain of TraI / Specimen concentration: 19.7 mg/ml
BufferName: 50 mM TRIS-HCl 100 mM NaCl / pH: 8
Entity #1426Name: Tra_2 / Type: protein / Description: Relaxase (Tra_2) domain of TraI / Formula weight: 46.027 / Num. of mol.: 1 / Source: Neisseria gonorrhoeae / References: UniProt: Q5EPC7
Sequence: MHHHHHHSTD SVSHNDEVAH INQNIVNDRF RILNAHELIQ VLDLSPQISG IKMNLGLSDE NWSKDALPFL EKYIAFVQRL PASESHHHAG DGGLVRHTLD VAALALVAST SQSWPPNAKT EEIAKKTAVW RYGIMCAALL HDVGKTVTGF QVELFDSAIS LEKLLWLPDT ...Sequence:
MHHHHHHSTD SVSHNDEVAH INQNIVNDRF RILNAHELIQ VLDLSPQISG IKMNLGLSDE NWSKDALPFL EKYIAFVQRL PASESHHHAG DGGLVRHTLD VAALALVAST SQSWPPNAKT EEIAKKTAVW RYGIMCAALL HDVGKTVTGF QVELFDSAIS LEKLLWLPDT GSMAESGKLY YRVEFPDAKS AYSTHAEIAW TFFQALVPSH VRQWLATTDP NLMITLRNYL SGKKDGSPLE QLIKNADMTS VSRDLRSGSR QRFSTAKRKP FIETIMETLK EMLSDRGVHF SIATTAGGDL FRKGDRIYLM SKNVPDYIRQ YLRKNQHPAA GSFPADNQRI FDTLFEYRAV IPPENDPHRA INHIEVEFTR MDGEKVRNIF SVLCFNAKTL YPDGDYPTEF LGNLSEV

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.867 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Relaxase domain of TraI / Measurement date: Jul 11, 2018 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 2 sec. / Number of frames: 1500 / Unit: 1/nm /
MinMax
Q0.0349 4.9595
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 596 /
MinMax
Q0.214518 3.02656
P(R) point1 596
R0 8.25
Result
Type of curve: sec
Comments: Please note: The protein contains unstructured regions that impart a level of flexibility to the overall structure in solution. As a consequence, the conformational state(s) sampled by the ...Comments: Please note: The protein contains unstructured regions that impart a level of flexibility to the overall structure in solution. As a consequence, the conformational state(s) sampled by the protein differs from - and cannot be captured by - the single predicted PHYRE2 atomistic model/representation displayed in this entry (as evidenced by the poor fit of the model to the SAXS data; bottom).
ExperimentalPorod
MW42.07 kDa47.44 kDa
Volume-60.74 nm3

P(R)GuinierGuinier error
Forward scattering, I075.59 74.67 0.07
Radius of gyration, Rg2.711 nm2.64 nm0.07

MinMax
D-8.25
Guinier point39 95

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