+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDE69 |
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試料 | Interleukin-1 receptor type 2, IL-1RII-ECD
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機能・相同性 | 機能・相同性情報 interleukin-1, type II, blocking receptor activity / negative regulation of interleukin-1 alpha production / interleukin-1 receptor activity / negative regulation of interleukin-1-mediated signaling pathway / interleukin-1 binding / negative regulation of protein processing / Interleukin-10 signaling / negative regulation of cytokine production involved in inflammatory response / protein processing / Interleukin-1 signaling ...interleukin-1, type II, blocking receptor activity / negative regulation of interleukin-1 alpha production / interleukin-1 receptor activity / negative regulation of interleukin-1-mediated signaling pathway / interleukin-1 binding / negative regulation of protein processing / Interleukin-10 signaling / negative regulation of cytokine production involved in inflammatory response / protein processing / Interleukin-1 signaling / immune response / extracellular region / plasma membrane / cytoplasm 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) |
引用 | ジャーナル: Structure / 年: 2019 タイトル: Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. 著者: Jiwan Ge / Soumya G Remesh / Michal Hammel / Si Pan / Andrew D Mahan / Shuying Wang / Xinquan Wang / 要旨: The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 ...The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 cytokines. The inter-domain flexibility of IL-1 receptors and its functional roles have not been fully elucidated. In this study, we used small-angle X-ray scattering to show that ligand-binding primary receptors and co-receptors in the family all have inherent inter-domain flexibility due to the D2/D3 linker. Variants of the IL-1RAcP and IL-18Rβ co-receptors with mutated D2/D3 linkers cannot form a cytokine-receptor complex and mediate signaling. Our analysis further revealed that these mutated co-receptors exhibited a changed conformational ensemble, suggesting that loss of function is due to the alteration of receptor dynamics. Taken together, our results demonstrate that the D2/D3 linker is a critical functional determinant of IL-1 receptor and underscore the important roles of the inter-domain flexibility in cytokine/receptor binding and signaling. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #2683 | タイプ: atomic / カイ2乗値: 1.91581367539 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #2684 | タイプ: atomic / カイ2乗値: 1.91581367539 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: Interleukin-1 receptor type 2, IL-1RII-ECD / 試料濃度: 10 mg/ml |
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バッファ | 名称: 10mM HEPES, 150mM NaCl, 3% glycerol / pH: 7.2 |
要素 #1394 | 名称: IL-1R-2 / タイプ: protein / 記述: Interleukin-1 receptor type 2 / 分子量: 38.351 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P27930 配列: FTLQPAAHTG AARSCRFRGR HYKREFRLEG EPVALRCPQV PYWLWASVSP RINLTWHKND SARTVPGEEE TRMWAQDGAL WLLPALQEDS GTYVCTTRNA SYCDKMSIEL RVFENTDAFL PFISYPQILT LSTSGVLVCP DLSEFTRDKT DVKIQWYKDS LLLDKDNEKF ...配列: FTLQPAAHTG AARSCRFRGR HYKREFRLEG EPVALRCPQV PYWLWASVSP RINLTWHKND SARTVPGEEE TRMWAQDGAL WLLPALQEDS GTYVCTTRNA SYCDKMSIEL RVFENTDAFL PFISYPQILT LSTSGVLVCP DLSEFTRDKT DVKIQWYKDS LLLDKDNEKF LSVRGTTHLL VHDVALEDAG YYRCVLTFAH EGQQYNITRS IELRIKKKKE ETIPVIISPL KTISASLGSR LTIPCKVFLG TGTPLTTMLW WTANDTHIES AYPGGRVTEG PRQEYSENNE NYIEVPLIFD PVTREDLHMD FKCVVHNTLS FQTLRTTVHH HHHH |
-実験情報
ビーム | 設備名称: Advanced Light Source (ALS) 12.3.1 (SIBYLS) / 地域: Berkeley, CA / 国: USA / 線源: X-ray synchrotron / 波長: 0.103 Å / スペクトロメータ・検出器間距離: 1.5 mm | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus3 X 2M / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
スキャン | タイトル: Interleukin-1 receptor type 2, IL-1RII-ECD / 測定日: 2017年7月24日 / セル温度: 20 °C / 照射時間: 3 sec. / フレーム数: 600 / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 460 /
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結果 | カーブのタイプ: sec コメント: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; ...コメント: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.
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