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- SASDEB8: Bacillus thuringiensis LexA repressor (Bt_LexA) (Bacillus thuring... -

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Basic information

Entry
Database: SASBDB / ID: SASDEB8
SampleBacillus thuringiensis LexA repressor (Bt_LexA)
  • Bacillus thuringiensis LexA repressor (protein), LexA, Bacillus thuringiensis
Function / homology
Function and homology information


repressor LexA / SOS response / DNA replication / serine-type endopeptidase activity / DNA repair / negative regulation of DNA-templated transcription / proteolysis / DNA binding
Similarity search - Function
LexA repressor, DNA-binding domain / Transcription regulator LexA / LexA DNA binding domain / Peptidase S24, LexA-like / LexA-like / Peptidase S24/S26A/S26B/S26C / Peptidase S24-like / LexA/Signal peptidase-like superfamily / ArsR-like helix-turn-helix domain / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
Biological speciesBacillus thuringiensis (bacteria)
CitationDate: 2019 May
Title: Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor
Authors: Caveney N / Pavlin A / Caballero G / Bahun M / Hodnik V / de Castro L / Fornelos N / Butala M
Contact author
  • Nathanael Caveney (University of British Columbia)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2673
Type: atomic / Comment: I-TASSER, CRYSOL, ModLoop model of LexA / Chi-square value: 2.428
Search similar-shape structures of this assembly by Omokage search (details)
Model #2674
Type: atomic
Comment: I-TASSER, CRYSOL, ModLoop model of LexA in SREFLEX, restrained
Chi-square value: 2.018 / P-value: 0.000002
Search similar-shape structures of this assembly by Omokage search (details)
Model #2675
Type: atomic
Comment: I-TASSER, CRYSOL, ModLoop model of LexA in SREFLEX, unrestrained
Chi-square value: 1.527 / P-value: 0.005267
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Bacillus thuringiensis LexA repressor (Bt_LexA) / Specimen concentration: 5.00-40.00
BufferName: 20 mM Hepes, 300 mM NaCl, 10% glycerol, / pH: 8
Entity #1376Name: LexA / Type: protein / Description: Bacillus thuringiensis LexA repressor / Formula weight: 23.394 / Num. of mol.: 2 / Source: Bacillus thuringiensis / References: UniProt: A0A2B4EEI3
Sequence: MLENMEKLTK RQQDILDFIK LKVQEKGYPP SVREIGQAVG LASSSTVHGH LSRLEEKGYI RRDPTKPRAI EILGEDRMDT ETQSVIQVPI VGKVTAGLPI TAVESVEEHF PLPASIVSGA DQVFMLRISG DSMIEAGIFD GDLVVVRQQQ SAYNGEIVVA LTEDNEATVK ...Sequence:
MLENMEKLTK RQQDILDFIK LKVQEKGYPP SVREIGQAVG LASSSTVHGH LSRLEEKGYI RRDPTKPRAI EILGEDRMDT ETQSVIQVPI VGKVTAGLPI TAVESVEEHF PLPASIVSGA DQVFMLRISG DSMIEAGIFD GDLVVVRQQQ SAYNGEIVVA LTEDNEATVK RFYKEKDHFR LQPENSSLEP IILKQVSVIG KVIGVYRDLH

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Experimental information

BeamInstrument name: University of British Columbia Rigaku BioSAXS-2000
City: Vancouver / 国: Canada / Type of source: X-ray in house / Wavelength: 0.154 Å
DetectorName: Rigaku HyOix-3000 / Type: Hybrid Pixel Array Detector / Pixsize x: 100 mm
Scan
Title: Bacillus thuringiensis LexA repressor (Bt_LexA) / Measurement date: Aug 25, 2017 / Storage temperature: 6 °C / Cell temperature: 6 °C / Exposure time: 300 sec. / Number of frames: 12 / Unit: 1/A /
MinMax
Q0.0084 0.6422
ResultType of curve: merged /
ExperimentalPorod
MW64.1 kDa69 kDa
Volume-110 nm3

GuinierGuinier error
Forward scattering, I00.94 0.005
Radius of gyration, Rg3.7 nm0.03

MinMax
Guinier point1 31

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