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- SASDEC8: Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDEC8
SampleBacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)
  • Bacillus thuringiensis LexA repressor (protein), LexA, Bacillus thuringiensis
  • Bacteriophage pGIL01 gp7 (protein), gp7, Bacteriophage pGIL01
Function / homology
Function and homology information


repressor LexA / SOS response / DNA replication / serine-type endopeptidase activity / DNA repair / negative regulation of DNA-templated transcription / proteolysis / DNA binding
Similarity search - Function
LexA repressor, DNA-binding domain / Transcription regulator LexA / LexA DNA binding domain / Peptidase S24, LexA-like / LexA-like / Peptidase S24/S26A/S26B/S26C / Peptidase S24-like / LexA/Signal peptidase-like superfamily / ArsR-like helix-turn-helix domain / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
LexA repressor / Transcriptional regulator
Similarity search - Component
Biological speciesBacillus thuringiensis (bacteria)
Bacteriophage pGIL01
CitationDate: 2019 May
Title: Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor
Authors: Caveney N / Pavlin A / Caballero G / Bahun M / Hodnik V / de Castro L / Fornelos N / Butala M
Contact author
  • Nathanael Caveney (University of British Columbia)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2676
Type: atomic
Comment: I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex
Chi-square value: 6.158
Search similar-shape structures of this assembly by Omokage search (details)
Model #2677
Type: atomic
Comment: I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, restrained
Chi-square value: 2.580 / P-value: 0.000002
Search similar-shape structures of this assembly by Omokage search (details)
Model #2678
Type: atomic
Comment: I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, unrestrained
Chi-square value: 1.899 / P-value: 0.000040
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)
Specimen concentration: 0.75-12.00 / Entity id: 1376 / 1377
BufferName: 20 mM Hepes, 300 mM NaCl, 10% glycerol, / pH: 8
Entity #1376Name: LexA / Type: protein / Description: Bacillus thuringiensis LexA repressor / Formula weight: 23.394 / Num. of mol.: 2 / Source: Bacillus thuringiensis / References: UniProt: A0A2B4EEI3
Sequence: MLENMEKLTK RQQDILDFIK LKVQEKGYPP SVREIGQAVG LASSSTVHGH LSRLEEKGYI RRDPTKPRAI EILGEDRMDT ETQSVIQVPI VGKVTAGLPI TAVESVEEHF PLPASIVSGA DQVFMLRISG DSMIEAGIFD GDLVVVRQQQ SAYNGEIVVA LTEDNEATVK ...Sequence:
MLENMEKLTK RQQDILDFIK LKVQEKGYPP SVREIGQAVG LASSSTVHGH LSRLEEKGYI RRDPTKPRAI EILGEDRMDT ETQSVIQVPI VGKVTAGLPI TAVESVEEHF PLPASIVSGA DQVFMLRISG DSMIEAGIFD GDLVVVRQQQ SAYNGEIVVA LTEDNEATVK RFYKEKDHFR LQPENSSLEP IILKQVSVIG KVIGVYRDLH
Entity #1377Name: gp7 / Type: protein / Description: Bacteriophage pGIL01 gp7 / Formula weight: 5.921 / Num. of mol.: 4 / Source: Bacteriophage pGIL01 / References: UniProt: Q7WSG2
Sequence:
MRDKLLDFII ELSQSSKQVV SKSYVIDRLM QVTKEDYKEL EKNVEGKKDD

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Experimental information

BeamInstrument name: University of British Columbia Rigaku BioSAXS-2000
City: Vancouver / 国: Canada / Type of source: X-ray in house / Wavelength: 0.154 Å
DetectorName: Rigaku HyOix-3000 / Type: Hybrid Pixel Array Detector / Pixsize x: 100 mm
Scan
Title: Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)
Measurement date: Aug 25, 2017 / Storage temperature: 6 °C / Cell temperature: 6 °C / Exposure time: 300 sec. / Number of frames: 12 / Unit: 1/A /
MinMax
Q0.0117 0.6422
ResultExperimental MW: 108.2 kDa / I0 from Guinier: 1.53 / Rg from Guinier: 4.391 nm / Type of curve: merged

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