SASDET5: Old Yellow Enzyme of Leishmania braziliensis (LbOYE)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDET5

試料

Old Yellow Enzyme of Leishmania braziliensis

• Old Yellow Enzyme of Leishmania braziliensis (protein), LbOYE, Leishmania braziliensis

• 生物種: Leishmania braziliensis (ブラジルリーシュマニア)
• 引用: ジャーナル: Arch Biochem Biophys / 年: 2019; タイトル: Structural studies of Old Yellow Enzyme of Leishmania braziliensis in solution.; 著者: Laudimir Leonardo Walbert Veloso-Silva / Paulo Roberto Dores-Silva / Dayane Eliara Bertolino-Reis / Louis Fellipe Moreno-Oliveira / Silvia Helena Libardi / Júlio César Borges / ; 要旨: First described in yeast in 1932 by Christian & Warburg, the Old Yellow Enzyme (OYE) (EC 1.6.99.1) has aroused the interest of the scientific community regarding its high ability to catalyze stereoselective reactions of α/β-unsaturated carbonyl compounds with important industrial applications. In addition, the OYE family of proteins has been found in different organisms, such as plants, bacteria and protozoa, but not in mammals, which makes it an excellent candidate for a functional and molecular study aimed at more effective therapies with fewer undesirable side effects. Several OYE orthologues have been characterized; however, the real physiological role for most members of this family of proteins remains a mystery. In this paper, we present the structural studies of the OYE of Leishmania braziliensis. The findings are discussed in comparison with OYE of Trypanosoma cruzi, revealing some biophysical differences. The main differences are related to their chemical and thermal stabilities and behavior in solution. In addition, the L. braziliensis OYE shape is more elongated than that of the T. cruzi orthologue. Despite this, the active sites of these enzymes do not appear to have major differences, since their interactions with the substrate menadione occur with an affinity of the same order of magnitude, revealing that the binding sites in both proteins are essentially similar.

登録者

• Júlio Borges (Instituto de Química de São Carlos, São Carlos - SP - Brasil)

モデル

• モデル #2480: ; タイプ: dummy / ダミー原子の半径: 2.40 A / カイ2乗値: 6.159; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Old Yellow Enzyme of Leishmania braziliensis / 試料濃度: 3 mg/ml
• バッファ: 名称: 25 mM Tris-HCl 100 mM NaCl and 1 mM β-mercaptoethanol; pH: 8

要素 #1325

名称: LbOYE / タイプ: protein / 記述: Old Yellow Enzyme of Leishmania braziliensis / 分子量: 41.859 / 分子数: 1 / 由来: Leishmania braziliensis
配列:

GSEFDYTMPT ASNSIDALLK PLVIGQLSMP NRFVMAPLTR CRADDEHVPT PAMVRHYADR ASMGLIITEA TQIEKGYSTF CHEGGIYGKE QVDGWRKVTD AVHEKGGLIF CQIHNGGRAT VPSNVDEGVR IVAPSAVAIT DHECPGAFAR NGEKQPYPVP HEMTAEDIAL YVKLYATAAC NAMAAGFDGV EVHGANGYLI DEFLKSSSNQ RTDEYGGSIE NRCRFLFEVL DAVIKAIGRE RVGLRISPLN SFNSQSDENP EALTRYICSQ LNSRNISFLD VMRGDFFSQA CGADKWAREA YEGVLFTGMG FEIEEAAETV ASGAADAVVF GTKALANPDL VARAIAGAAL NTPDPATFYS TSEAGYNDYP SMVSSCPSAA APSCTKETP

実験情報

• ビーム: 設備名称: Brazilian Synchrotron Light Laboratory SAXS1 Beamline; 地域: Campinas / 国: Brazil / 線源: X-ray synchrotron / 波長: 0.148 Å / スペクトロメータ・検出器間距離: 1 mm
• 検出器: 名称: Pilatus 300K / タイプ: 20Hz

スキャン

タイトル: Old Yellow Enzyme of Leishmania braziliensis / 測定日: 2015年5月26日 / 保管温度: 4 °C / セル温度: 25 °C / 照射時間: 360 sec. / フレーム数: 1 / 単位: 1/A /

	Min	Max
Q	0.0234	0.3181

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 234 /

	Min	Max
Q	0.02344	0.3181
P(R) point	1	234
R	0	108

結果

カーブのタイプ: single_conc

	実験値	Standard	Standard error	Porod
分子量	40 kDa	48.6 kDa	4	40 kDa
体積	-	-	-	73 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.3768	0.037	0.381	0.0007
慣性半径, Rg	2.69 nm	0.2	2.71 nm	-

	Min	Max	Error
D	-	9.5	1
Guinier point	1	16	-