+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDAJ6 |
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Sample | WbdD(1-556)
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Citation | Journal: Nat Struct Mol Biol / Year: 2015 Title: A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide. Authors: Gregor Hagelueken / Bradley R Clarke / Hexian Huang / Anne Tuukkanen / Iulia Danciu / Dmitri I Svergun / Rohanah Hussain / Huanting Liu / Chris Whitfield / James H Naismith / Abstract: Long-chain bacterial polysaccharides have important roles in pathogenicity. In Escherichia coli O9a, a model for ABC transporter-dependent polysaccharide assembly, a large extracellular carbohydrate ...Long-chain bacterial polysaccharides have important roles in pathogenicity. In Escherichia coli O9a, a model for ABC transporter-dependent polysaccharide assembly, a large extracellular carbohydrate with a narrow size distribution is polymerized from monosaccharides by a complex of two proteins, WbdA (polymerase) and WbdD (terminating protein). Combining crystallography and small-angle X-ray scattering, we found that the C-terminal domain of WbdD contains an extended coiled-coil that physically separates WbdA from the catalytic domain of WbdD. The effects of insertions and deletions in the coiled-coil region were analyzed in vivo, revealing that polymer size is controlled by varying the length of the coiled-coil domain. Thus, the coiled-coil domain of WbdD functions as a molecular ruler that, along with WbdA:WbdD stoichiometry, controls the chain length of a model bacterial polysaccharide. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #190 | Type: atomic / Software: CORAL / Radius of dummy atoms: 1.90 A / Chi-square value: 1.0816 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #191 | Type: dummy / Software: DAMMIN / Radius of dummy atoms: 1.90 A Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: WbdD(1-556) / Contrast: 3.047 / Dry vol: 76600 / Specimen concentration: 1.00-10.00 / Concentration method: Nanodrop |
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Buffer | Name: BisTris / Concentration: 20.00 mM / pH: 7 / Composition: 50 mM NaCl, 5 mM DTT |
Entity #127 | Name: WbdD / Type: protein / Description: bifunctional kinase- methyltransferase WbdD / Formula weight: 63.3 / Num. of mol.: 3 |
-Experimental information
Beam | Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm | |||||||||||||||||||||
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Detector | Name: Pilatus 1M-W / Pixsize x: 0.172 mm | |||||||||||||||||||||
Scan | Title: WbdD(1-556) / Measurement date: Dec 2, 2011 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 15 sec. / Number of frames: 10 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 388 /
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Result | D max: 17 / Type of curve: merged
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