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TitleA coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide.
Journal, issue, pagesNat Struct Mol Biol, Vol. 22, Issue 1, Page 50-56, Year 2015
Publish dateDec 15, 2014
AuthorsGregor Hagelueken / Bradley R Clarke / Hexian Huang / Anne Tuukkanen / Iulia Danciu / Dmitri I Svergun / Rohanah Hussain / Huanting Liu / Chris Whitfield / James H Naismith /
PubMed AbstractLong-chain bacterial polysaccharides have important roles in pathogenicity. In Escherichia coli O9a, a model for ABC transporter-dependent polysaccharide assembly, a large extracellular carbohydrate ...Long-chain bacterial polysaccharides have important roles in pathogenicity. In Escherichia coli O9a, a model for ABC transporter-dependent polysaccharide assembly, a large extracellular carbohydrate with a narrow size distribution is polymerized from monosaccharides by a complex of two proteins, WbdA (polymerase) and WbdD (terminating protein). Combining crystallography and small-angle X-ray scattering, we found that the C-terminal domain of WbdD contains an extended coiled-coil that physically separates WbdA from the catalytic domain of WbdD. The effects of insertions and deletions in the coiled-coil region were analyzed in vivo, revealing that polymer size is controlled by varying the length of the coiled-coil domain. Thus, the coiled-coil domain of WbdD functions as a molecular ruler that, along with WbdA:WbdD stoichiometry, controls the chain length of a model bacterial polysaccharide.
External linksNat Struct Mol Biol / PubMed:25504321 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution3.87 Å
Structure data

SASDAH6:
WbdD(1-459) (bifunctional kinase- methyltransferase WbdD, WbdD)
Method: SAXS/SANS

SASDAJ6:
WbdD(1-556) (bifunctional kinase- methyltransferase WbdD, WbdD)
Method: SAXS/SANS

PDB-4uw0:
Low resolution structure of WbdD with C-terminal bundle ordered to residue 505
Method: X-RAY DIFFRACTION / Resolution: 3.87 Å

Chemicals

ChemComp-SAM:
S-ADENOSYLMETHIONINE

Source
  • escherichia coli (E. coli)
KeywordsTRANSFERASE / KINASE / METHYLTRANSFERASE

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