SASDCU8: Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium therm...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDCU8

試料

Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum

• hypothetical protein CTHT_0072540 (protein), Chaetomium thermophilum

機能・相同性

分子機能:

Prp19 complex / protein K63-linked ubiquitination / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / ubiquitin protein ligase activity / DNA repair

ドメイン・相同性:

Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / U-box domain profile. / Modified RING finger domain / U-box domain / Zinc finger, RING/FYVE/PHD-type / Trp-Asp (WD) repeats profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

構成要素:

Pre-mRNA-processing factor 19

• 生物種: Chaetomium thermophilum (菌類)
• 引用: ジャーナル: Mol Cell / 年: 2018; タイトル: Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.; 著者: Tales Rocha de Moura / Sina Mozaffari-Jovin / Csaba Zoltán Kibédi Szabó / Jana Schmitzová / Olexandr Dybkov / Constantin Cretu / Michael Kachala / Dmitri Svergun / Henning Urlaub / Reinhard Lührmann / Vladimir Pena / ; 要旨: Human nineteen complex (NTC) acts as a multimeric E3 ubiquitin ligase in DNA repair and splicing. The transfer of ubiquitin is mediated by Prp19-a homotetrameric component of NTC whose elongated coiled coils serve as an assembly axis for two other proteins called SPF27 and CDC5L. We find that Prp19 is inactive on its own and have elucidated the structural basis of its autoinhibition by crystallography and mutational analysis. Formation of the NTC core by stepwise assembly of SPF27, CDC5L, and PLRG1 onto the Prp19 tetramer enables ubiquitin ligation. Protein-protein crosslinking of NTC, functional assays in vitro, and assessment of its role in DNA damage response provide mechanistic insight into the organization of the NTC core and the communication between PLRG1 and Prp19 that enables E3 activity. This reveals a unique mode of regulation for a complex E3 ligase and advances understanding of its dynamics in various cellular pathways.

登録者

• Tales Rocha de Moura (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #1466: ; タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 1.331; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #1467: ; タイプ: dummy / ダミー原子の半径: 2.00 A / カイ2乗値: 0.957; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum; 試料濃度: 1.50-5.00
• バッファ: 名称: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol / pH: 7.5

要素 #761

タイプ: protein / 記述: hypothetical protein CTHT_0072540 / 分子量: 34.583 / 分子数: 1 / 由来: Chaetomium thermophilum / 参照: UniProt: G0SFY0
配列:

SLSEGLVEHV NEVQQQLMKT RKKRPIPQGW ATADDVAALQ QVAYTDLNVT QASSLDLENE CAAVGGLDGK LDIYSVVANK VERTLDIGEP VTATEWTGTK VVIGTAKGWV KVYDAGRESA TFQTHAGPVT GLAVHPGGRI LASVGVDKSF VFYDLETGER VARGYADAAL TTCAFHPDGN LFAAGTQTGH ILVFHTTTLE QAESFPLGTP IQALAFSENG FWFAATGKGT SSVTIFDLRK SGAAAAVKEL QTGEVLSISW DYTGQYLATG GGTGVTVQMY TKATKSWSEP VRLGMPVVGV KWGGEAKRLV VVSREGVVSV LGKKEE

実験情報

• ビーム: 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.124 Å / スペクトロメータ・検出器間距離: 3.1 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum
測定日: 2013年7月19日 / セル温度: 25 °C / 照射時間: 1 sec. / フレーム数: 20 / 単位: 1/nm /

	Min	Max
Q	0.1828	2.8112

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 1000 /

	Min	Max
Q	0.182778	2.81125
P(R) point	1	1000
R	0	5.8

結果

カーブのタイプ: other

	実験値	Standard	Porod
分子量	40.8 kDa	56 kDa	40.8 kDa
体積	-	-	68 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	1376	9	1477.17	23.5
慣性半径, Rg	2.15 nm	0.009	2.3 nm	0.09

	Min	Max	Error
D	-	5.8	6
Guinier point	1	97	-