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- SASDCV8: Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Cha... -

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Basic information

Entry
Database: SASBDB / ID: SASDCV8
SampleUncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum
  • Full-length hypothetical protein CTHT_0072540 (protein), Chaetomium thermophilum
Function / homology
Function and homology information


Prp19 complex / protein K63-linked ubiquitination / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / ubiquitin protein ligase activity / DNA repair
Similarity search - Function
Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / U-box domain profile. / Modified RING finger domain / U-box domain / Zinc finger, RING/FYVE/PHD-type / Trp-Asp (WD) repeats profile. / WD domain, G-beta repeat / WD40 repeats ...Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / U-box domain profile. / Modified RING finger domain / U-box domain / Zinc finger, RING/FYVE/PHD-type / Trp-Asp (WD) repeats profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Pre-mRNA-processing factor 19
Similarity search - Component
Biological speciesChaetomium thermophilum (fungus)
CitationJournal: Mol Cell / Year: 2018
Title: Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.
Authors: Tales Rocha de Moura / Sina Mozaffari-Jovin / Csaba Zoltán Kibédi Szabó / Jana Schmitzová / Olexandr Dybkov / Constantin Cretu / Michael Kachala / Dmitri Svergun / Henning Urlaub / ...Authors: Tales Rocha de Moura / Sina Mozaffari-Jovin / Csaba Zoltán Kibédi Szabó / Jana Schmitzová / Olexandr Dybkov / Constantin Cretu / Michael Kachala / Dmitri Svergun / Henning Urlaub / Reinhard Lührmann / Vladimir Pena /
Abstract: Human nineteen complex (NTC) acts as a multimeric E3 ubiquitin ligase in DNA repair and splicing. The transfer of ubiquitin is mediated by Prp19-a homotetrameric component of NTC whose elongated ...Human nineteen complex (NTC) acts as a multimeric E3 ubiquitin ligase in DNA repair and splicing. The transfer of ubiquitin is mediated by Prp19-a homotetrameric component of NTC whose elongated coiled coils serve as an assembly axis for two other proteins called SPF27 and CDC5L. We find that Prp19 is inactive on its own and have elucidated the structural basis of its autoinhibition by crystallography and mutational analysis. Formation of the NTC core by stepwise assembly of SPF27, CDC5L, and PLRG1 onto the Prp19 tetramer enables ubiquitin ligation. Protein-protein crosslinking of NTC, functional assays in vitro, and assessment of its role in DNA damage response provide mechanistic insight into the organization of the NTC core and the communication between PLRG1 and Prp19 that enables E3 activity. This reveals a unique mode of regulation for a complex E3 ligase and advances understanding of its dynamics in various cellular pathways.
Contact author
  • Tales Rocha de Moura (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1468
Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 1.555
Search similar-shape structures of this assembly by Omokage search (details)
Model #1469
Type: dummy / Software: (r9988) / Radius of dummy atoms: 3.60 A / Chi-square value: 1.003 / P-value: 0.030000
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum
Specimen concentration: 0.60-4.40
BufferName: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol / pH: 7.5
Entity #763Type: protein / Description: Full-length hypothetical protein CTHT_0072540 / Formula weight: 51.788 / Num. of mol.: 4 / Source: Chaetomium thermophilum / References: UniProt: G0SFY0
Sequence: MLCALSGEIP EEPVVSKKTG VLFEKRLILK YLEEHNNIEP GTTEELDPET DLLPIKTSRV VRPRPPNFTS IPSLLKAFQD EWDALVLETY TTREQLARVR EELATALYQH DAAVRVIARL TRERDEAREA LARLTVTGAA PAAQNGEAMA VDSESLSEGL VEHVNEVQQQ ...Sequence:
MLCALSGEIP EEPVVSKKTG VLFEKRLILK YLEEHNNIEP GTTEELDPET DLLPIKTSRV VRPRPPNFTS IPSLLKAFQD EWDALVLETY TTREQLARVR EELATALYQH DAAVRVIARL TRERDEAREA LARLTVTGAA PAAQNGEAMA VDSESLSEGL VEHVNEVQQQ LMKTRKKRPI PQGWATADDV AALQQVAYTD LNVTQASSLD LENECAAVGG LDGKLDIYSV VANKVERTLD IGEPVTATEW TGTKVVIGTA KGWVKVYDAG RESATFQTHA GPVTGLAVHP GGRILASVGV DKSFVFYDLE TGERVARGYA DAALTTCAFH PDGNLFAAGT QTGHILVFHT TTLEQAESFP LGTPIQALAF SENGFWFAAT GKGTSSVTIF DLRKSGAAAA VKELQTGEVL SISWDYTGQY LATGGGTGVT VQMYTKATKS WSEPVRLGMP VVGVKWGGEA KRLVVVSREG VVSVLGKKEE

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Experimental information

BeamInstrument name: MAX IV I911-4 / City: Lund / 国: Sweden / Type of source: X-ray synchrotron / Wavelength: 0.091 Å / Dist. spec. to detc.: 2.2 mm
DetectorName: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm
Scan
Title: Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum
Measurement date: Oct 15, 2013 / Cell temperature: 25 °C / Exposure time: 120 sec. / Unit: 1/nm /
MinMax
Q0.1425 2.9981
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 263 /
MinMax
Q0.1561 2.998
P(R) point3 265
R0 23
Result
Type of curve: merged
ExperimentalStandardPorod
MW220 kDa220 kDa175 kDa
Volume--280 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.0008299 1.6E-5 0.00084 6.7E-5
Radius of gyration, Rg6.73 nm0.097 6.24 nm0.54

MinMaxError
D-23 3.6
Guinier point3 13 -

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