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基本情報
登録情報 | データベース: SASBDB / ID: SASDC66 |
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![]() | YtvA (lit/active state)
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機能・相同性 | ![]() |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: The switch that does not flip: the blue-light receptor YtvA from Bacillus subtilis adopts an elongated dimer conformation independent of the activation state as revealed by a combined AUC and SAXS study. 著者: Marcel Jurk / Matthias Dorn / Alexey Kikhney / Dmitri Svergun / Wolfgang Gärtner / Peter Schmieder / ![]() 要旨: Photoreceptors play an important role in plants and bacteria by converting extracellular stimuli into intracellular signals. One distinct class are the blue-light-sensitive phototropins harboring a ...Photoreceptors play an important role in plants and bacteria by converting extracellular stimuli into intracellular signals. One distinct class are the blue-light-sensitive phototropins harboring a light-oxygen-voltage (LOV) domain coupled to various effector domains. Photon absorption by the chromophore within the LOV domain results in an activation of the output domain via mechanisms that are hitherto not well understood. The photoreceptor YtvA from Bacillus subtilis is a bacterial analog of phototropins, consists of an LOV and a sulfate transporter/anti-sigma factor antagonist domain, and is involved in the response of the bacterium to environmental stress. We present here analytical ultracentrifugation studies and small-angle X-ray scattering experiments, showing that YtvA is a dimer. On the basis of these results, we present a low-resolution model of the dimer in the dark and the lit state of the protein. In addition, we show that YtvA does not change its oligomerization state or its overall shape upon light activation. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #1445 | ![]() タイプ: dummy / ダミー原子の半径: 1.90 A / 対称性: P2 / カイ2乗値: 9.07 ![]() |
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モデル #1444 | ![]() タイプ: dummy / ダミー原子の半径: 2.20 A / 対称性: P2 / カイ2乗値: 7.896 ![]() |
モデル #1446 | ![]() タイプ: dummy / ダミー原子の半径: 3.25 A / カイ2乗値: 7.896 ![]() |
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試料
![]() | 名称: YtvA (lit/active state) / 試料濃度: 1.70-11.00 |
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バッファ | 名称: PBS + 5 mM DTT / pH: 7.4 コメント: 10 mM phosphate buffer; 2.7 mM KCl; 137 mM NaCl; 5 mM DTT |
要素 #731 | 名称: YtvA / タイプ: protein 記述: YtvA (pfyP - Blue light photoreceptor - Bacillus subtilis (strain 168) 分子量: 29.12 / 分子数: 2 / 由来: Escherichia coli / 参照: UniProt: O34627 配列: GASFQSFGIP GQLEVIKKAL DHVRVGVVIT DPALEDNPIV YVNQGFVQMT GYETEEILGK NCRFLQGKHT DPAEVDNIRT ALQNKEPVTV QIQNYKKDGT MFWNELNIDP MEIEDKTYFV GIQNDITKQK EYEKLLEDSL TEITALSTPI VPIRNGISAL PLVGNLTEER ...配列: GASFQSFGIP GQLEVIKKAL DHVRVGVVIT DPALEDNPIV YVNQGFVQMT GYETEEILGK NCRFLQGKHT DPAEVDNIRT ALQNKEPVTV QIQNYKKDGT MFWNELNIDP MEIEDKTYFV GIQNDITKQK EYEKLLEDSL TEITALSTPI VPIRNGISAL PLVGNLTEER FNSIVCTLTN ILSTSKDDYL IIDLSGLAQV NEQTADQIFK LSHLLKLTGT ELIITGIKPE LAMKMNKLDA NFSSLKTYSN VKDAVKVLPI M |
-実験情報
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検出器 | 名称: Pilatus 1M-W / Pixsize x: 0.172 mm | |||||||||||||||||||||||||||||||||
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距離分布関数 P(R) |
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