SASDC96: N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maq...

Basic information

• Entry: Database: SASBDB / ID: SASDC96

Sample

N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C

• Diguanylate cyclase with PAS/PAC sensor (protein), Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

Function / homology

Function:

negative regulation of bacterial-type flagellum-dependent cell motility / diguanylate cyclase / diguanylate cyclase activity / cell adhesion involved in single-species biofilm formation / plasma membrane

Domain/homology:

: / PAS fold-3 / PAS fold / Diguanylate cyclase, GGDEF domain / diguanylate cyclase / GGDEF domain profile. / GGDEF domain / PAS-associated, C-terminal / PAC domain profile. / Nucleotide cyclase / PAC motif / Motif C-terminal to PAS motifs (likely to contribute to PAS structural domain) / PAS domain / PAS domain superfamily / Reverse transcriptase/Diguanylate cyclase domain

Component:

diguanylate cyclase

• Biological species: Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8) (bacteria)
• Citation: Journal: Biopolymers / Year: 2011; Title: Small angle X-ray scattering as a complementary tool for high-throughput structural studies.; Authors: Thomas D Grant / Joseph R Luft / Jennifer R Wolfley / Hiro Tsuruta / Anne Martel / Gaetano T Montelione / Edward H Snell / ; Abstract: Structural crystallography and nuclear magnetic resonance (NMR) spectroscopy are the predominant techniques for understanding the biological world on a molecular level. Crystallography is constrained by the ability to form a crystal that diffracts well and NMR is constrained to smaller proteins. Although powerful techniques, they leave many soluble, purified structurally uncharacterized protein samples. Small angle X-ray scattering (SAXS) is a solution technique that provides data on the size and multiple conformations of a sample, and can be used to reconstruct a low-resolution molecular envelope of a macromolecule. In this study, SAXS has been used in a high-throughput manner on a subset of 28 proteins, where structural information is available from crystallographic and/or NMR techniques. These crystallographic and NMR structures were used to validate the accuracy of molecular envelopes reconstructed from SAXS data on a statistical level, to compare and highlight complementary structural information that SAXS provides, and to leverage biological information derived by crystallographers and spectroscopists from their structures. All the ab initio molecular envelopes calculated from the SAXS data agree well with the available structural information. SAXS is a powerful albeit low-resolution technique that can provide additional structural information in a high-throughput and complementary manner to improve the functional interpretation of high-resolution structures.

Models

• Model #1411: ; Type: dummy / Radius of dummy atoms: 1.75 A / Chi-square value: 1.833316; Search similar-shape structures of this assembly by Omokage search (details)
• Model #1415: ; Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 6.796449; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C; Specimen concentration: 2.57-7.08
• Buffer: Name: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3 / pH: 7.5

Entity #743

Type: protein / Description: Diguanylate cyclase with PAS/PAC sensor / Formula weight: 13.601 / Num. of mol.: 2
Source: Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
References: UniProt: A1U4R9
Sequence:

MTKAIPWKIN WQTMAFEYIG PQIEALLGWP QGSWKSVEDW ATRMHPEDQE WVVNFCVKQS ECGVDHEADY RALHRDGHYV WIRDVVHVVR DDSGEVEALI GFMFDISLEH HHHHH

Experimental information

• Beam: Instrument name: Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2; City: Stanford, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.13 Å / Dist. spec. to detc.: 1.5 mm
• Detector: Name: Rayonix MX225-HE

Scan

Title: N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C
Measurement date: Feb 12, 2010 / Storage temperature: -80 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 20 / Unit: 1/A /

	Min	Max
Q	0.0159	0.3496

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 349 /

	Min	Max
Q	0.02524	0.3496
P(R) point	10	358
R	0	67.43

Result

D max: 6.74 / Type of curve: single_conc /

	Experimental	Porod
MW	24.56 kDa	24.56 kDa
Volume	-	40.76 nm3

	P(R)	Guinier
Forward scattering, I0	1197	1191.41
Radius of gyration, Rg	1.98 nm	1.97 nm