SASDCQ6: Rap guanine nucleotide exchange factor 3 (isoform3) - apo form

Basic information

• Entry: Database: SASBDB / ID: SASDCQ6

Sample

Rap guanine nucleotide exchange factor 3 (isoform3) - apo form

• Rap guanine nucleotide exchange factor 3 (protein), EPAC1B, Homo sapiens

• Function / homology: Isoform 3 of Rap guanine nucleotide exchange factor 3
• Biological species: Homo sapiens (human)

Models

• Model #1143: ; Type: atomic / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Chi-square value: 1.0404 / P-value: 0.799000; Search similar-shape structures of this assembly by Omokage search (details)
• Model #1146: ; Type: dummy / Radius of dummy atoms: 3.50 A / Symmetry: P1 / Comment: SAXNS_DAMMIF / Chi-square value: 1.022121 / P-value: 0.713900; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Rap guanine nucleotide exchange factor 3 (isoform3) - apo form; Specimen concentration: 0.50-0.50
• Buffer: Name: 1mM EDTA, 10mM DTT, 500mM NaCl, and 10mM Tris / pH: 9

Entity #595

Name: EPAC1B / Type: protein / Description: Rap guanine nucleotide exchange factor 3 / Formula weight: 100.001 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: O95398-3
Sequence:

GSPGIRVLVL RRMHRPRSCS YQLLLEHQRP SCIQGLRWTP LTNSEESLDF SESLEQASTE RVLRAGRQLH RHLLATCPNL IRDRKYHLRL YRQCCSGREL VDGILALGLG VHSRSQVVGI CQVLLDEGAL CHVKHDWAFQ DRDAQFYRFP GPEPEPVGTH EMEEELAEAV ALLSQRGPDA LLTVALRKPP GQRTDEELDL IFEELLHIKA VAHLSNSVKR ELAAVLLFEP HSKAGTVLFS QGDKGTSWYI IWKGSVNVVT HGKGLVTTLH EGDDFGQLAL VNDAPRAATI ILREDNCHFL RVDKQDFNRI IKDVEAKTMR LEEHGKVVLV LERASQGAGP SRPPTPGRNR YTVMSGTPEK ILELLLEAMG PDSSAHDPTE TFLSDFLLTH RVFMPSAQLC AALLHHFHVE PAGGSEQERS TYVCNKRQQI LRLVSQWVAL YGSMLHTDPV ATSFLQKLSD LVGRDTRLSN LLREQWPERR RCHRLENGCG NASPQMKARN LPVWLPNQDE PLPGSSCAIQ VGDKVPYDIC RPDHSVLTLQ LPVTASVREV MAALAQEDGW TKGQVLVKVN SAGDAIGLQP DARGVATSLG LNERLFVVNP QEVHELIPHP DQLGPTVGSA EGLDLVSAKD LAGQLTDHDW SLFNSIHQVE LIHYVLGPQH LRDVTTANLE RFMRRFNELQ YWVATELCLC PVPGPRAQLL RKFIKLAAHL KEQKNLNSFF AVMFGLSNSA ISRLAHTWER LPHKVRKLYS ALERLLDPSW NHRVYRLALA KLSPPVIPFM PLLLKDMTFI HEGNHTLVEN LINFEKMRMM ARAARMLHHC RSHNPVPLSP LRSRVSHLHE DSQVARISTC SEQSLSTRSP ASTWAYVQQL KVIDNQRELS RLSRELEP

Experimental information

• Beam: Instrument name: Sealy Center For Structural Biology, UTMB-G Rigaku BioSAXS-1000; City: Galveston, TX / 国: USA / Type of source: X-ray in house / Wavelength: 1.5418 Å / Dist. spec. to detc.: 0.489 mm
• Detector: Name: Rigaku BioSAXS-1000 / Type: Kratky-2D / Pixsize x: 0.001 mm

Scan

Title: Rap guanine nucleotide exchange factor 3 (isoform3) - apo form
Measurement date: Sep 7, 2012 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 21600 sec. / Number of frames: 12 / Unit: 1/A /

	Min	Max
Q	0.0108	0.6753

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 157 /

	Min	Max
Q	0.01512	0.2376
P(R) point	4	160
R	0	109.9

Result

Type of curve: single_conc

	Experimental	Standard	Porod
MW	100 kDa	73 kDa	113 kDa
Volume	-	-	180 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.2183	0.0012	0.2168	0.002
Radius of gyration, Rg	3.41 nm	0.02	3.35 nm	5

	Min	Max
D	-	11
Guinier point	1	19