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- SASDB89: Telomeric DNA duplex (telomere DNA duplex) -

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Basic information

Entry
Database: SASBDB / ID: SASDB89
SampleTelomeric DNA duplex
  • telomere DNA duplex (DNA)
CitationDate: 2017 Sep 13
Title: Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it
Authors: Nečasová I / Janoušková E / Klumpler T
Contact author
  • Tomas Klumpler (CEITEC - Central European Institute of Technology, Masaryk University)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #1105
Type: dummy / Software: (145) / Radius of dummy atoms: 2.25 A / Chi-square value: 1.218 / P-value: 0.002600
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Telomeric DNA duplex / Specimen concentration: 1.4 mg/ml
BufferName: 20 mM Tris-HCl, 50 mM LiCl / pH: 7.5
Entity #570Type: DNA / Description: telomere DNA duplex / Formula weight: 10.522 / Num. of mol.: 1
Sequence:
GTTAGGGTTA GGGTTAGCAA TCCCAATCCC AATC

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Experimental information

BeamInstrument name: CEITEC Rigaku BioSAXS-1000 / City: Brno / 国: Czech Republic / Type of source: X-ray in house / Wavelength: 0.154 Å / Dist. spec. to detc.: 0.4791 mm
DetectorName: Pilatus 100K / Pixsize x: 172 mm
Scan
Title: Telomeric DNA duplex / Measurement date: May 3, 2016 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 60 sec. / Number of frames: 6 / Unit: 1/A /
MinMax
Q0.034 0.3004
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 185 /
MinMax
Q0.0354019 0.300378
P(R) point1 185
R0 57.1
Result
Type of curve: single_conc
Comments: Refer to the related SASBDB entries SASDB79 and SASDC62.
ExperimentalPorod
MW10.5 kDa-
Volume-11.9 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.09753 0.001077 0.094 0.001
Radius of gyration, Rg1.709 nm0.024 1.588 nm0.035

MinMax
D-5.71
Guinier point2 34

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