PDB-9v7i: Phycobilisome rod R3 from Gloeobacter violaceus PCC 7421

基本情報

• 登録情報: データベース: PDB / ID: 9v7i
• タイトル: Phycobilisome rod R3 from Gloeobacter violaceus PCC 7421

要素

• (Phycobilisome rod linker polypeptide ...) x 2
• Glr1262 protein
• Phycocyanin alpha chain
• Phycocyanin beta chain

• キーワード: PHOTOSYNTHESIS / Phycobilisome

機能・相同性

分子機能:

phycobilisome / plasma membrane-derived thylakoid membrane / photosynthesis

ドメイン・相同性:

Phycobilisome linker domain / Phycobilisome linker domain superfamily / Phycobilisome Linker polypeptide / Phycobilisome (PBS) linker domain profile. / Phycobilisome, alpha/beta subunit / Phycobilisome, alpha/beta subunit superfamily / Phycobilisome protein / Globin-like superfamily

構成要素:

PHYCOCYANOBILIN / Phycocyanin beta chain / Phycocyanin alpha chain / Glr1262 protein

• 生物種: Gloeobacter violaceus PCC 7421 (バクテリア)
• 手法: 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.03 Å
• データ登録者: Burtseva, A.D. / Baymukhametov, T.N. / Slonimskiy, Y.B. / Popov, V.O. / Sluchanko, N.N. / Boyko, K.M.

資金援助

ロシア, 1件

組織	認可番号	国
Russian Science Foundation	23-74-00062	ロシア

• 引用: ジャーナル: Sci Adv / 年: 2025; タイトル: Structure and quenching of a bundle-shaped phycobilisome.; 著者: Anna D Burtseva / Yury B Slonimskiy / Timur N Baymukhametov / Maria A Sinetova / Daniil A Gvozdev / Georgy V Tsoraev / Dmitry A Cherepanov / Eugene G Maksimov / Vladimir O Popov / Konstantin M Boyko / Nikolai N Sluchanko / ; 要旨: Cyanobacteria use soluble antenna megacomplexes, phycobilisomes (PBSs), to maximize light-harvesting efficiency and small photoswitchable orange carotenoid proteins (OCPs) to down-regulate PBSs in high light. Among known PBS morphologies, the one from the basal cyanobacterial genus still lacks detailed structural characterization. Here, we reconstructed a cryo-electron microscopy structure of the >10-megadalton PBS, with diverging, conformationally mobile bundles of rods composed of stacked phycoerythrin and phycocyanin hexamers, stemming from a pentacylindrical allophycocyanin core belted by auxiliary phycocyanin hexamers. We show how two -specific multidomain linker proteins, Glr1262 and Glr2806, maintain this bundle-shaped architecture and reveal its differential regulation via nonphotochemical quenching by two OCP types of that recognize separate binding sites within the allophycocyanin core, including lateral cylinders absent in tricylindrical cores.

履歴

登録	2025年5月28日	登録サイト: PDBJ / 処理サイト: PDBJ
改定 1.0	2025年9月24日	Provider: repository / タイプ: Initial release
改定 1.0	2025年9月24日	Data content type: EM metadata / Data content type: EM metadata / Provider: repository / タイプ: Initial release
改定 1.0	2025年9月24日	Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0	2025年9月24日	Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / タイプ: Initial release
改定 1.0	2025年9月24日	Data content type: Image / Data content type: Image / Provider: repository / タイプ: Initial release
改定 1.0	2025年9月24日	Data content type: Primary map / Data content type: Primary map / Provider: repository / タイプ: Initial release
改定 1.1	2025年10月29日	Group: Data collection / Database references / カテゴリ: citation / citation_author / em_admin Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _em_admin.last_update

集合体

登録構造単位

A: Phycobilisome rod linker polypeptide CpcC1

B: Phycocyanin beta chain

C: Phycocyanin alpha chain

D: Phycocyanin beta chain

E: Phycocyanin alpha chain

F: Phycocyanin beta chain

G: Phycocyanin alpha chain

H: Phycocyanin beta chain

I: Phycocyanin alpha chain

J: Phycocyanin beta chain

K: Phycocyanin alpha chain

L: Phycocyanin beta chain

M: Phycobilisome rod linker polypeptide CpcC2

N: Phycocyanin alpha chain

O: Phycocyanin beta chain

P: Phycocyanin alpha chain

Q: Phycocyanin beta chain

R: Phycocyanin alpha chain

S: Phycocyanin beta chain

T: Phycocyanin alpha chain

U: Phycocyanin beta chain

V: Phycocyanin alpha chain

W: Phycocyanin beta chain

X: Phycocyanin alpha chain

Y: Phycocyanin beta chain

a: Phycocyanin alpha chain

Z: Glr1262 protein

ヘテロ分子

概要:

• 609 kDa, 27 ポリマー

構成要素の詳細:

	分子量 (理論値)	分子数
合計 (水以外)	609,225	63
ポリマ－	588,032	27
非ポリマー	21,193	36
水	0	0

1

概要:

• 登録構造と同一
• 登録者が定義した集合体
• 根拠: 電子顕微鏡法, not applicable

対称操作:

タイプ	名称	対称操作	数
identity operation	1_555		1

非結晶学的対称性 (NCS)

NCSドメイン:

ID	Ens-ID	詳細
1	1	A
2	1	A
3	2	A
4	2	A
5	3	A
6	3	A
7	4	A
8	4	A
9	5	A
10	5	A
11	6	A
12	6	A
13	7	A
14	7	A
15	8	A
16	8	A
17	9	A
18	9	A
19	10	A
20	10	A
21	11	A
22	11	A
23	12	A
24	12	A
25	13	A
26	13	A
27	14	A
28	14	A
29	15	A
30	15	A
31	16	A
32	16	A
33	17	A
34	17	A
35	18	A
36	18	A
37	19	A
38	19	A
39	20	A
40	20	A
41	21	A
42	21	A
43	22	A
44	22	A
45	23	A
46	23	A
47	24	A
48	24	A
49	25	A
50	25	A
51	26	A
52	26	A
53	27	A
54	27	A
55	28	A
56	28	A
57	29	A
58	29	A
59	30	A
60	30	A
61	31	A
62	31	A
63	32	A
64	32	A
65	33	A
66	33	A
67	34	A
68	34	A
69	35	A
70	35	A
71	36	A
72	36	A
73	37	A
74	37	A
75	38	A
76	38	A
77	39	A
78	39	A
79	40	A
80	40	A
81	41	A
82	41	A
83	42	A
84	42	A
85	43	A
86	43	A
87	44	A
88	44	A
89	45	A
90	45	A
91	46	A
92	46	A
93	47	A
94	47	A
95	48	A
96	48	A
97	49	A
98	49	A
99	50	A
100	50	A
101	51	A
102	51	A
103	52	A
104	52	A
105	53	A
106	53	A
107	54	A
108	54	A
109	55	A
110	55	A
111	56	A
112	56	A
113	57	A
114	57	A
115	58	A
116	58	A
117	59	A
118	59	A
119	60	A
120	60	A
121	61	A
122	61	A
123	62	A
124	62	A
125	63	A
126	63	A
127	64	A
128	64	A
129	65	A
130	65	A
131	66	A
132	66	A
133	67	A
134	67	A
135	68	A
136	68	A
137	69	A
138	69	A
139	70	A
140	70	A
141	71	A
142	71	A
143	72	A
144	72	A
145	73	A
146	73	A
147	74	A
148	74	A
149	75	A
150	75	A
151	76	A
152	76	A
153	77	A
154	77	A
155	78	A
156	78	A
157	79	A
158	79	A
159	80	A
160	80	A
161	81	A
162	81	A
163	82	A
164	82	A
165	83	A
166	83	A
167	84	A
168	84	A
169	85	A
170	85	A
171	86	A
172	86	A
173	87	A
174	87	A
175	88	A
176	88	A
177	89	A
178	89	A
179	90	A
180	90	A
181	91	A
182	91	A
183	92	A
184	92	A
185	93	A
186	93	A
187	94	A
188	94	A
189	95	A
190	95	A
191	96	A
192	96	A
193	97	A
194	97	A
195	98	A
196	98	A
197	99	A
198	99	A
199	100	A
200	100	A
201	101	A
202	101	A
203	102	A
204	102	A
205	103	A
206	103	A
207	104	A
208	104	A
209	105	A
210	105	A
211	106	A
212	106	A
213	107	A
214	107	A
215	108	A
216	108	A
217	109	A
218	109	A
219	110	A
220	110	A
221	111	A
222	111	A
223	112	A
224	112	A
225	113	A
226	113	A
227	114	A
228	114	A
229	115	A
230	115	A
231	116	A
232	116	A
233	117	A
234	117	A
235	118	A
236	118	A
237	119	A
238	119	A
239	120	A
240	120	A
241	121	A
242	121	A
243	122	A
244	122	A
245	123	A
246	123	A
247	124	A
248	124	A
249	125	A
250	125	A
251	126	A
252	126	A
253	127	A
254	127	A
255	128	A
256	128	A
257	129	A
258	129	A
259	130	A
260	130	A
261	131	A
262	131	A
263	132	A
264	132	A

NCSアンサンブル:

ID	詳細
1	Local NCS retraints between domains: 1 2
2	Local NCS retraints between domains: 3 4
3	Local NCS retraints between domains: 5 6
4	Local NCS retraints between domains: 7 8
5	Local NCS retraints between domains: 9 10
6	Local NCS retraints between domains: 11 12
7	Local NCS retraints between domains: 13 14
8	Local NCS retraints between domains: 15 16
9	Local NCS retraints between domains: 17 18
10	Local NCS retraints between domains: 19 20
11	Local NCS retraints between domains: 21 22
12	Local NCS retraints between domains: 23 24
13	Local NCS retraints between domains: 25 26
14	Local NCS retraints between domains: 27 28
15	Local NCS retraints between domains: 29 30
16	Local NCS retraints between domains: 31 32
17	Local NCS retraints between domains: 33 34
18	Local NCS retraints between domains: 35 36
19	Local NCS retraints between domains: 37 38
20	Local NCS retraints between domains: 39 40
21	Local NCS retraints between domains: 41 42
22	Local NCS retraints between domains: 43 44
23	Local NCS retraints between domains: 45 46
24	Local NCS retraints between domains: 47 48
25	Local NCS retraints between domains: 49 50
26	Local NCS retraints between domains: 51 52
27	Local NCS retraints between domains: 53 54
28	Local NCS retraints between domains: 55 56
29	Local NCS retraints between domains: 57 58
30	Local NCS retraints between domains: 59 60
31	Local NCS retraints between domains: 61 62
32	Local NCS retraints between domains: 63 64
33	Local NCS retraints between domains: 65 66
34	Local NCS retraints between domains: 67 68
35	Local NCS retraints between domains: 69 70
36	Local NCS retraints between domains: 71 72
37	Local NCS retraints between domains: 73 74
38	Local NCS retraints between domains: 75 76
39	Local NCS retraints between domains: 77 78
40	Local NCS retraints between domains: 79 80
41	Local NCS retraints between domains: 81 82
42	Local NCS retraints between domains: 83 84
43	Local NCS retraints between domains: 85 86
44	Local NCS retraints between domains: 87 88
45	Local NCS retraints between domains: 89 90
46	Local NCS retraints between domains: 91 92
47	Local NCS retraints between domains: 93 94
48	Local NCS retraints between domains: 95 96
49	Local NCS retraints between domains: 97 98
50	Local NCS retraints between domains: 99 100
51	Local NCS retraints between domains: 101 102
52	Local NCS retraints between domains: 103 104
53	Local NCS retraints between domains: 105 106
54	Local NCS retraints between domains: 107 108
55	Local NCS retraints between domains: 109 110
56	Local NCS retraints between domains: 111 112
57	Local NCS retraints between domains: 113 114
58	Local NCS retraints between domains: 115 116
59	Local NCS retraints between domains: 117 118
60	Local NCS retraints between domains: 119 120
61	Local NCS retraints between domains: 121 122
62	Local NCS retraints between domains: 123 124
63	Local NCS retraints between domains: 125 126
64	Local NCS retraints between domains: 127 128
65	Local NCS retraints between domains: 129 130
66	Local NCS retraints between domains: 131 132
67	Local NCS retraints between domains: 133 134
68	Local NCS retraints between domains: 135 136
69	Local NCS retraints between domains: 137 138
70	Local NCS retraints between domains: 139 140
71	Local NCS retraints between domains: 141 142
72	Local NCS retraints between domains: 143 144
73	Local NCS retraints between domains: 145 146
74	Local NCS retraints between domains: 147 148
75	Local NCS retraints between domains: 149 150
76	Local NCS retraints between domains: 151 152
77	Local NCS retraints between domains: 153 154
78	Local NCS retraints between domains: 155 156
79	Local NCS retraints between domains: 157 158
80	Local NCS retraints between domains: 159 160
81	Local NCS retraints between domains: 161 162
82	Local NCS retraints between domains: 163 164
83	Local NCS retraints between domains: 165 166
84	Local NCS retraints between domains: 167 168
85	Local NCS retraints between domains: 169 170
86	Local NCS retraints between domains: 171 172
87	Local NCS retraints between domains: 173 174
88	Local NCS retraints between domains: 175 176
89	Local NCS retraints between domains: 177 178
90	Local NCS retraints between domains: 179 180
91	Local NCS retraints between domains: 181 182
92	Local NCS retraints between domains: 183 184
93	Local NCS retraints between domains: 185 186
94	Local NCS retraints between domains: 187 188
95	Local NCS retraints between domains: 189 190
96	Local NCS retraints between domains: 191 192
97	Local NCS retraints between domains: 193 194
98	Local NCS retraints between domains: 195 196
99	Local NCS retraints between domains: 197 198
100	Local NCS retraints between domains: 199 200
101	Local NCS retraints between domains: 201 202
102	Local NCS retraints between domains: 203 204
103	Local NCS retraints between domains: 205 206
104	Local NCS retraints between domains: 207 208
105	Local NCS retraints between domains: 209 210
106	Local NCS retraints between domains: 211 212
107	Local NCS retraints between domains: 213 214
108	Local NCS retraints between domains: 215 216
109	Local NCS retraints between domains: 217 218
110	Local NCS retraints between domains: 219 220
111	Local NCS retraints between domains: 221 222
112	Local NCS retraints between domains: 223 224
113	Local NCS retraints between domains: 225 226
114	Local NCS retraints between domains: 227 228
115	Local NCS retraints between domains: 229 230
116	Local NCS retraints between domains: 231 232
117	Local NCS retraints between domains: 233 234
118	Local NCS retraints between domains: 235 236
119	Local NCS retraints between domains: 237 238
120	Local NCS retraints between domains: 239 240
121	Local NCS retraints between domains: 241 242
122	Local NCS retraints between domains: 243 244
123	Local NCS retraints between domains: 245 246
124	Local NCS retraints between domains: 247 248
125	Local NCS retraints between domains: 249 250
126	Local NCS retraints between domains: 251 252
127	Local NCS retraints between domains: 253 254
128	Local NCS retraints between domains: 255 256
129	Local NCS retraints between domains: 257 258
130	Local NCS retraints between domains: 259 260
131	Local NCS retraints between domains: 261 262
132	Local NCS retraints between domains: 263 264

要素

Phycobilisome rod linker polypeptide ... , 2種, 2分子 A M

#1: タンパク質

A Phycobilisome rod linker polypeptide CpcC1

詳細:

分子量: 31085.066 Da / 分子数: 1 / 由来タイプ: 天然
由来: (天然) Gloeobacter violaceus PCC 7421 (バクテリア)

#4: タンパク質

M Phycobilisome rod linker polypeptide CpcC2

詳細:

分子量: 30913.822 Da / 分子数: 1 / 由来タイプ: 天然
由来: (天然) Gloeobacter violaceus PCC 7421 (バクテリア)

タンパク質 , 3種, 25分子 B D F H J L O Q S U W Y C E G I K N P R T V X a Z

#2: タンパク質

B D F H J L O Q S U W Y
Phycocyanin beta chain

詳細:

分子量: 18478.953 Da / 分子数: 12 / 由来タイプ: 天然
由来: (天然) Gloeobacter violaceus PCC 7421 (バクテリア)
参照: UniProt: Q7M7C7

#3: タンパク質

C E G I K N P R T V X a
Phycocyanin alpha chain

詳細:

分子量: 17679.852 Da / 分子数: 12 / 由来タイプ: 天然
由来: (天然) Gloeobacter violaceus PCC 7421 (バクテリア)
参照: UniProt: Q7M7F7

#5: タンパク質

Z Glr1262 protein

詳細:

分子量: 92127.602 Da / 分子数: 1 / 由来タイプ: 天然
由来: (天然) Gloeobacter violaceus PCC 7421 (バクテリア)
参照: UniProt: Q7NL64

非ポリマー , 1種, 36分子

#6: 化合物

B-201 B-202 C-201 D-201 D-202 E-201 F-201 F-202 G-201 H-201 H-202 I-201 J-201 J-202 K-201 L-201 L-202 N-201 O-201 O-202 ...
ChemComp-CYC / PHYCOCYANOBILIN

詳細:

分子量: 588.694 Da / 分子数: 36 / 由来タイプ: 合成 / 式: C₃₃H₄₀N₄O₆ / タイプ: SUBJECT OF INVESTIGATION

詳細

• 研究の焦点であるリガンドがあるか: Y
• Has protein modification: Y

実験情報

実験

• 実験: 手法: 電子顕微鏡法
• EM実験: 試料の集合状態: PARTICLE / ３次元再構成法: 単粒子再構成法

試料調製

• 構成要素: 名称: Bundle-shaped phycobilisome / タイプ: COMPLEX / Entity ID: #1-#5 / 由来: NATURAL
• 由来(天然): 生物種: Gloeobacter violaceus PCC 7421 (バクテリア)
• 緩衝液: pH: 7
• 緩衝液成分: 濃度: 50 mM / 名称: Tris(hydroxymethyl)aminomethane hydrochloride / 式: Tris-HCl
• 試料: 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES
• 急速凍結: 凍結剤: ETHANE

電子顕微鏡撮影

• 実験機器: モデル: Titan Krios / 画像提供: FEI Company
• 顕微鏡: モデル: TFS KRIOS / 詳細: Preliminary grid screening was performed manually.
• 電子銃: 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM
• 電子レンズ: モード: BRIGHT FIELD / 倍率（公称値）: 85000 X / 最大 デフォーカス（公称値）: 1600 nm / 最小 デフォーカス（公称値）: 600 nm / Cs: 0.01 mm / C2レンズ絞り径: 100 µm / アライメント法: ZEMLIN TABLEAU
• 試料ホルダ: 凍結剤: NITROGEN; 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
• 撮影: 平均露光時間: 3.9 sec. / 電子線照射量: 66 e/Ų; フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k)
• 電子光学装置: エネルギーフィルター名称: GIF Bioquantum / エネルギーフィルタースリット幅: 20 eV; 球面収差補正装置: Microscope was modified with a Cs corrector (CEOS GmbH, Germany).
• 画像スキャン: 横: 5760 / 縦: 4092

解析

EMソフトウェア

ID	名称	バージョン	カテゴリ
2	SerialEM	4.04	画像取得
4	Warp	1.0.9	CTF補正
11	cryoSPARC	4.7	最終オイラー角割当
13	cryoSPARC	4.7	３次元再構成

• CTF補正: タイプ: NONE
• 対称性: 点対称性: C₁ (非対称)
• 3次元再構成: 解像度: 3.03 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 746972 / 対称性のタイプ: POINT

精密化

解像度: 3.03→3.03 Å / Cor.coef. F_o:F_c: 0.964 / WR_factor R_work: 0.328 / SU B: 35.894 / SU ML: 0.582 / Average fsc free: 0 / Average fsc overall: 0.7546 / Average fsc work: 0.7546 / ESU R: 0.824 / 詳細: Hydrogens have been added in their riding positions

	Rfactor	反射数	%反射
Rwork	0.3276	202863	-
all	0.328	-	-
Rfree	-	-	0 %
obs	-	-	100 %

• 溶媒の処理: 溶媒モデル: NONE
• 原子変位パラメータ: B_iso mean: 55.44 Ų

拘束条件

Refine-ID	タイプ	Dev ideal	Dev ideal target	数
ELECTRON MICROSCOPY	r_bond_refined_d	0.007	0.012	37105
ELECTRON MICROSCOPY	r_bond_other_d	0.001	0.016	35189
ELECTRON MICROSCOPY	r_angle_refined_deg	1.645	1.815	50490
ELECTRON MICROSCOPY	r_angle_other_deg	0.636	1.749	80506
ELECTRON MICROSCOPY	r_dihedral_angle_1_deg	6.18	5	4540
ELECTRON MICROSCOPY	r_dihedral_angle_2_deg	16.278	8.522	920
ELECTRON MICROSCOPY	r_dihedral_angle_3_deg	17.249	10	5935
ELECTRON MICROSCOPY	r_dihedral_angle_6_deg	13.904	10	1611
ELECTRON MICROSCOPY	r_chiral_restr	0.069	0.2	5598
ELECTRON MICROSCOPY	r_gen_planes_refined	0.007	0.02	45915
ELECTRON MICROSCOPY	r_gen_planes_other	0.004	0.02	8591
ELECTRON MICROSCOPY	r_nbd_refined	0.312	0.2	11608
ELECTRON MICROSCOPY	r_symmetry_nbd_other	0.223	0.2	34503
ELECTRON MICROSCOPY	r_nbtor_refined	0.204	0.2	19829
ELECTRON MICROSCOPY	r_symmetry_nbtor_other	0.083	0.2	20460
ELECTRON MICROSCOPY	r_xyhbond_nbd_refined	0.173	0.2	1274
ELECTRON MICROSCOPY	r_symmetry_xyhbond_nbd_other	0.099	0.2	45
ELECTRON MICROSCOPY	r_mcbond_it	1.409	5.604	18241
ELECTRON MICROSCOPY	r_mcbond_other	1.409	5.604	18241
ELECTRON MICROSCOPY	r_mcangle_it	2.428	10.054	22754
ELECTRON MICROSCOPY	r_mcangle_other	2.428	10.054	22755
ELECTRON MICROSCOPY	r_scbond_it	1.33	5.594	18864
ELECTRON MICROSCOPY	r_scbond_other	1.33	5.594	18863
ELECTRON MICROSCOPY	r_scangle_it	2.428	10.261	27736
ELECTRON MICROSCOPY	r_scangle_other	2.428	10.261	27737
ELECTRON MICROSCOPY	r_lrange_it	4.351	50.585	46873
ELECTRON MICROSCOPY	r_lrange_other	4.351	50.585	46874
ELECTRON MICROSCOPY	r_ncsr_local_group_1	0.109	0.05	5276
ELECTRON MICROSCOPY	r_ncsr_local_group_2	0.079	0.05	5355
ELECTRON MICROSCOPY	r_ncsr_local_group_3	0.071	0.05	5430
ELECTRON MICROSCOPY	r_ncsr_local_group_4	0.08	0.05	5375
ELECTRON MICROSCOPY	r_ncsr_local_group_5	0.1	0.05	5307
ELECTRON MICROSCOPY	r_ncsr_local_group_6	0.047	0.05	5499
ELECTRON MICROSCOPY	r_ncsr_local_group_7	0.083	0.05	5387
ELECTRON MICROSCOPY	r_ncsr_local_group_8	0.081	0.05	5349
ELECTRON MICROSCOPY	r_ncsr_local_group_9	0.071	0.05	5427
ELECTRON MICROSCOPY	r_ncsr_local_group_10	0.08	0.05	5376
ELECTRON MICROSCOPY	r_ncsr_local_group_11	0.101	0.05	5286
ELECTRON MICROSCOPY	r_ncsr_local_group_12	0.063	0.05	5247
ELECTRON MICROSCOPY	r_ncsr_local_group_13	0.066	0.05	5272
ELECTRON MICROSCOPY	r_ncsr_local_group_14	0.083	0.05	5224
ELECTRON MICROSCOPY	r_ncsr_local_group_15	0.069	0.05	5251
ELECTRON MICROSCOPY	r_ncsr_local_group_16	0.091	0.05	5217
ELECTRON MICROSCOPY	r_ncsr_local_group_17	0.061	0.05	5290
ELECTRON MICROSCOPY	r_ncsr_local_group_18	0.062	0.05	5248
ELECTRON MICROSCOPY	r_ncsr_local_group_19	0.063	0.05	5286
ELECTRON MICROSCOPY	r_ncsr_local_group_20	0.087	0.05	5221
ELECTRON MICROSCOPY	r_ncsr_local_group_21	0.058	0.05	5272
ELECTRON MICROSCOPY	r_ncsr_local_group_22	0.09	0.05	5232
ELECTRON MICROSCOPY	r_ncsr_local_group_23	0.105	0.05	5244
ELECTRON MICROSCOPY	r_ncsr_local_group_24	0.106	0.05	5313
ELECTRON MICROSCOPY	r_ncsr_local_group_25	0.112	0.05	5261
ELECTRON MICROSCOPY	r_ncsr_local_group_26	0.112	0.05	5283
ELECTRON MICROSCOPY	r_ncsr_local_group_27	0.118	0.05	5258
ELECTRON MICROSCOPY	r_ncsr_local_group_28	0.081	0.05	5438
ELECTRON MICROSCOPY	r_ncsr_local_group_29	0.104	0.05	5261
ELECTRON MICROSCOPY	r_ncsr_local_group_30	0.105	0.05	5313
ELECTRON MICROSCOPY	r_ncsr_local_group_31	0.112	0.05	5262
ELECTRON MICROSCOPY	r_ncsr_local_group_32	0.109	0.05	5282
ELECTRON MICROSCOPY	r_ncsr_local_group_33	0.064	0.05	5280
ELECTRON MICROSCOPY	r_ncsr_local_group_34	0.078	0.05	5225
ELECTRON MICROSCOPY	r_ncsr_local_group_35	0.06	0.05	5300
ELECTRON MICROSCOPY	r_ncsr_local_group_36	0.085	0.05	5243
ELECTRON MICROSCOPY	r_ncsr_local_group_37	0.078	0.05	5219
ELECTRON MICROSCOPY	r_ncsr_local_group_38	0.012	0.05	5374
ELECTRON MICROSCOPY	r_ncsr_local_group_39	0.06	0.05	5290
ELECTRON MICROSCOPY	r_ncsr_local_group_40	0.083	0.05	5222
ELECTRON MICROSCOPY	r_ncsr_local_group_41	0.052	0.05	5299
ELECTRON MICROSCOPY	r_ncsr_local_group_42	0.086	0.05	5241
ELECTRON MICROSCOPY	r_ncsr_local_group_43	0.073	0.05	5395
ELECTRON MICROSCOPY	r_ncsr_local_group_44	0.068	0.05	5380
ELECTRON MICROSCOPY	r_ncsr_local_group_45	0.099	0.05	5295
ELECTRON MICROSCOPY	r_ncsr_local_group_46	0.087	0.05	5344
ELECTRON MICROSCOPY	r_ncsr_local_group_47	0.08	0.05	5344
ELECTRON MICROSCOPY	r_ncsr_local_group_48	0.029	0.05	5480
ELECTRON MICROSCOPY	r_ncsr_local_group_49	0.072	0.05	5395
ELECTRON MICROSCOPY	r_ncsr_local_group_50	0.069	0.05	5381
ELECTRON MICROSCOPY	r_ncsr_local_group_51	0.099	0.05	5304
ELECTRON MICROSCOPY	r_ncsr_local_group_52	0.088	0.05	5218
ELECTRON MICROSCOPY	r_ncsr_local_group_53	0.067	0.05	5276
ELECTRON MICROSCOPY	r_ncsr_local_group_54	0.087	0.05	5228
ELECTRON MICROSCOPY	r_ncsr_local_group_55	0.082	0.05	5239
ELECTRON MICROSCOPY	r_ncsr_local_group_56	0.062	0.05	5284
ELECTRON MICROSCOPY	r_ncsr_local_group_57	0.031	0.05	5354
ELECTRON MICROSCOPY	r_ncsr_local_group_58	0.09	0.05	5226
ELECTRON MICROSCOPY	r_ncsr_local_group_59	0.056	0.05	5289
ELECTRON MICROSCOPY	r_ncsr_local_group_60	0.087	0.05	5243
ELECTRON MICROSCOPY	r_ncsr_local_group_61	0.076	0.05	5413
ELECTRON MICROSCOPY	r_ncsr_local_group_62	0.1	0.05	5333
ELECTRON MICROSCOPY	r_ncsr_local_group_63	0.079	0.05	5395
ELECTRON MICROSCOPY	r_ncsr_local_group_64	0.082	0.05	5426
ELECTRON MICROSCOPY	r_ncsr_local_group_65	0.075	0.05	5382
ELECTRON MICROSCOPY	r_ncsr_local_group_66	0.006	0.05	5575
ELECTRON MICROSCOPY	r_ncsr_local_group_67	0.075	0.05	5411
ELECTRON MICROSCOPY	r_ncsr_local_group_68	0.101	0.05	5336
ELECTRON MICROSCOPY	r_ncsr_local_group_69	0.09	0.05	5219
ELECTRON MICROSCOPY	r_ncsr_local_group_70	0.095	0.05	5206
ELECTRON MICROSCOPY	r_ncsr_local_group_71	0.096	0.05	5185
ELECTRON MICROSCOPY	r_ncsr_local_group_72	0.077	0.05	5224
ELECTRON MICROSCOPY	r_ncsr_local_group_73	0.085	0.05	5231
ELECTRON MICROSCOPY	r_ncsr_local_group_74	0.052	0.05	5334
ELECTRON MICROSCOPY	r_ncsr_local_group_75	0.083	0.05	5233
ELECTRON MICROSCOPY	r_ncsr_local_group_76	0.095	0.05	5201
ELECTRON MICROSCOPY	r_ncsr_local_group_77	0.102	0.05	5332
ELECTRON MICROSCOPY	r_ncsr_local_group_78	0.088	0.05	5341
ELECTRON MICROSCOPY	r_ncsr_local_group_79	0.089	0.05	5384
ELECTRON MICROSCOPY	r_ncsr_local_group_80	0.074	0.05	5364
ELECTRON MICROSCOPY	r_ncsr_local_group_81	0.076	0.05	5412
ELECTRON MICROSCOPY	r_ncsr_local_group_82	0.006	0.05	5563
ELECTRON MICROSCOPY	r_ncsr_local_group_83	0.099	0.05	5337
ELECTRON MICROSCOPY	r_ncsr_local_group_84	0.091	0.05	5227
ELECTRON MICROSCOPY	r_ncsr_local_group_85	0.063	0.05	5292
ELECTRON MICROSCOPY	r_ncsr_local_group_86	0.06	0.05	5299
ELECTRON MICROSCOPY	r_ncsr_local_group_87	0.064	0.05	5291
ELECTRON MICROSCOPY	r_ncsr_local_group_88	0.093	0.05	5221
ELECTRON MICROSCOPY	r_ncsr_local_group_89	0.04	0.05	5355
ELECTRON MICROSCOPY	r_ncsr_local_group_90	0.091	0.05	5240
ELECTRON MICROSCOPY	r_ncsr_local_group_91	0.106	0.05	5303
ELECTRON MICROSCOPY	r_ncsr_local_group_92	0.094	0.05	5361
ELECTRON MICROSCOPY	r_ncsr_local_group_93	0.101	0.05	5286
ELECTRON MICROSCOPY	r_ncsr_local_group_94	0.099	0.05	5335
ELECTRON MICROSCOPY	r_ncsr_local_group_95	0.102	0.05	5338
ELECTRON MICROSCOPY	r_ncsr_local_group_96	0.07	0.05	5432
ELECTRON MICROSCOPY	r_ncsr_local_group_97	0.099	0.05	5198
ELECTRON MICROSCOPY	r_ncsr_local_group_98	0.084	0.05	5241
ELECTRON MICROSCOPY	r_ncsr_local_group_99	0.086	0.05	5234
ELECTRON MICROSCOPY	r_ncsr_local_group_100	0.098	0.05	5203
ELECTRON MICROSCOPY	r_ncsr_local_group_101	0.087	0.05	5226
ELECTRON MICROSCOPY	r_ncsr_local_group_102	0.02	0.05	5380
ELECTRON MICROSCOPY	r_ncsr_local_group_103	0.093	0.05	5353
ELECTRON MICROSCOPY	r_ncsr_local_group_104	0.088	0.05	5344
ELECTRON MICROSCOPY	r_ncsr_local_group_105	0.079	0.05	5396
ELECTRON MICROSCOPY	r_ncsr_local_group_106	0.087	0.05	5342
ELECTRON MICROSCOPY	r_ncsr_local_group_107	0.111	0.05	5260
ELECTRON MICROSCOPY	r_ncsr_local_group_108	0.078	0.05	5224
ELECTRON MICROSCOPY	r_ncsr_local_group_109	0.08	0.05	5240
ELECTRON MICROSCOPY	r_ncsr_local_group_110	0.083	0.05	5263
ELECTRON MICROSCOPY	r_ncsr_local_group_111	0.074	0.05	5234
ELECTRON MICROSCOPY	r_ncsr_local_group_112	0.099	0.05	5202
ELECTRON MICROSCOPY	r_ncsr_local_group_113	0.081	0.05	5346
ELECTRON MICROSCOPY	r_ncsr_local_group_114	0.082	0.05	5431
ELECTRON MICROSCOPY	r_ncsr_local_group_115	0.089	0.05	5386
ELECTRON MICROSCOPY	r_ncsr_local_group_116	0.092	0.05	5371
ELECTRON MICROSCOPY	r_ncsr_local_group_117	0.059	0.05	5292
ELECTRON MICROSCOPY	r_ncsr_local_group_118	0.081	0.05	5227
ELECTRON MICROSCOPY	r_ncsr_local_group_119	0.052	0.05	5299
ELECTRON MICROSCOPY	r_ncsr_local_group_120	0.084	0.05	5244
ELECTRON MICROSCOPY	r_ncsr_local_group_121	0.075	0.05	5383
ELECTRON MICROSCOPY	r_ncsr_local_group_122	0.074	0.05	5364
ELECTRON MICROSCOPY	r_ncsr_local_group_123	0.1	0.05	5284
ELECTRON MICROSCOPY	r_ncsr_local_group_124	0.089	0.05	5228
ELECTRON MICROSCOPY	r_ncsr_local_group_125	0.054	0.05	5309
ELECTRON MICROSCOPY	r_ncsr_local_group_126	0.085	0.05	5254
ELECTRON MICROSCOPY	r_ncsr_local_group_127	0.075	0.05	5414
ELECTRON MICROSCOPY	r_ncsr_local_group_128	0.1	0.05	5346
ELECTRON MICROSCOPY	r_ncsr_local_group_129	0.085	0.05	5234
ELECTRON MICROSCOPY	r_ncsr_local_group_130	0.099	0.05	5202
ELECTRON MICROSCOPY	r_ncsr_local_group_131	0.099	0.05	5339
ELECTRON MICROSCOPY	r_ncsr_local_group_132	0.087	0.05	5244

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Refine-ID	タイプ	Rms dev position (Å)	Weight position
1	1	A	ELECTRON MICROSCOPY	Local ncs	0.10942	0.05008
1	2	A	ELECTRON MICROSCOPY	Local ncs	0.10942	0.05008
2	3	A	ELECTRON MICROSCOPY	Local ncs	0.07902	0.05009
2	4	A	ELECTRON MICROSCOPY	Local ncs	0.07902	0.05009
3	5	A	ELECTRON MICROSCOPY	Local ncs	0.07116	0.05009
3	6	A	ELECTRON MICROSCOPY	Local ncs	0.07116	0.05009
4	7	A	ELECTRON MICROSCOPY	Local ncs	0.08027	0.05009
4	8	A	ELECTRON MICROSCOPY	Local ncs	0.08027	0.05009
5	9	A	ELECTRON MICROSCOPY	Local ncs	0.09998	0.05008
5	10	A	ELECTRON MICROSCOPY	Local ncs	0.09998	0.05008
6	11	A	ELECTRON MICROSCOPY	Local ncs	0.04689	0.05009
6	12	A	ELECTRON MICROSCOPY	Local ncs	0.04689	0.05009
7	13	A	ELECTRON MICROSCOPY	Local ncs	0.08306	0.05009
7	14	A	ELECTRON MICROSCOPY	Local ncs	0.08306	0.05009
8	15	A	ELECTRON MICROSCOPY	Local ncs	0.08067	0.05009
8	16	A	ELECTRON MICROSCOPY	Local ncs	0.08067	0.05009
9	17	A	ELECTRON MICROSCOPY	Local ncs	0.07127	0.05009
9	18	A	ELECTRON MICROSCOPY	Local ncs	0.07127	0.05009
10	19	A	ELECTRON MICROSCOPY	Local ncs	0.08002	0.05009
10	20	A	ELECTRON MICROSCOPY	Local ncs	0.08002	0.05009
11	21	A	ELECTRON MICROSCOPY	Local ncs	0.10133	0.05008
11	22	A	ELECTRON MICROSCOPY	Local ncs	0.10133	0.05008
12	23	A	ELECTRON MICROSCOPY	Local ncs	0.0635	0.05009
12	24	A	ELECTRON MICROSCOPY	Local ncs	0.0635	0.05009
13	25	A	ELECTRON MICROSCOPY	Local ncs	0.06586	0.0501
13	26	A	ELECTRON MICROSCOPY	Local ncs	0.06586	0.0501
14	27	A	ELECTRON MICROSCOPY	Local ncs	0.08257	0.05009
14	28	A	ELECTRON MICROSCOPY	Local ncs	0.08257	0.05009
15	29	A	ELECTRON MICROSCOPY	Local ncs	0.06853	0.05009
15	30	A	ELECTRON MICROSCOPY	Local ncs	0.06853	0.05009
16	31	A	ELECTRON MICROSCOPY	Local ncs	0.09119	0.05009
16	32	A	ELECTRON MICROSCOPY	Local ncs	0.09119	0.05009
17	33	A	ELECTRON MICROSCOPY	Local ncs	0.06087	0.05009
17	34	A	ELECTRON MICROSCOPY	Local ncs	0.06087	0.05009
18	35	A	ELECTRON MICROSCOPY	Local ncs	0.06195	0.05009
18	36	A	ELECTRON MICROSCOPY	Local ncs	0.06195	0.05009
19	37	A	ELECTRON MICROSCOPY	Local ncs	0.06271	0.0501
19	38	A	ELECTRON MICROSCOPY	Local ncs	0.06271	0.0501
20	39	A	ELECTRON MICROSCOPY	Local ncs	0.08679	0.05009
20	40	A	ELECTRON MICROSCOPY	Local ncs	0.08679	0.05009
21	41	A	ELECTRON MICROSCOPY	Local ncs	0.0579	0.0501
21	42	A	ELECTRON MICROSCOPY	Local ncs	0.0579	0.0501
22	43	A	ELECTRON MICROSCOPY	Local ncs	0.09029	0.05009
22	44	A	ELECTRON MICROSCOPY	Local ncs	0.09029	0.05009
23	45	A	ELECTRON MICROSCOPY	Local ncs	0.1052	0.05008
23	46	A	ELECTRON MICROSCOPY	Local ncs	0.1052	0.05008
24	47	A	ELECTRON MICROSCOPY	Local ncs	0.10575	0.05008
24	48	A	ELECTRON MICROSCOPY	Local ncs	0.10575	0.05008
25	49	A	ELECTRON MICROSCOPY	Local ncs	0.11169	0.05008
25	50	A	ELECTRON MICROSCOPY	Local ncs	0.11169	0.05008
26	51	A	ELECTRON MICROSCOPY	Local ncs	0.11203	0.05008
26	52	A	ELECTRON MICROSCOPY	Local ncs	0.11203	0.05008
27	53	A	ELECTRON MICROSCOPY	Local ncs	0.11779	0.05007
27	54	A	ELECTRON MICROSCOPY	Local ncs	0.11779	0.05007
28	55	A	ELECTRON MICROSCOPY	Local ncs	0.08113	0.05008
28	56	A	ELECTRON MICROSCOPY	Local ncs	0.08113	0.05008
29	57	A	ELECTRON MICROSCOPY	Local ncs	0.10375	0.05008
29	58	A	ELECTRON MICROSCOPY	Local ncs	0.10375	0.05008
30	59	A	ELECTRON MICROSCOPY	Local ncs	0.10503	0.05008
30	60	A	ELECTRON MICROSCOPY	Local ncs	0.10503	0.05008
31	61	A	ELECTRON MICROSCOPY	Local ncs	0.11193	0.05008
31	62	A	ELECTRON MICROSCOPY	Local ncs	0.11193	0.05008
32	63	A	ELECTRON MICROSCOPY	Local ncs	0.10872	0.05008
32	64	A	ELECTRON MICROSCOPY	Local ncs	0.10872	0.05008
33	65	A	ELECTRON MICROSCOPY	Local ncs	0.06431	0.0501
33	66	A	ELECTRON MICROSCOPY	Local ncs	0.06431	0.0501
34	67	A	ELECTRON MICROSCOPY	Local ncs	0.07801	0.05009
34	68	A	ELECTRON MICROSCOPY	Local ncs	0.07801	0.05009
35	69	A	ELECTRON MICROSCOPY	Local ncs	0.06024	0.05009
35	70	A	ELECTRON MICROSCOPY	Local ncs	0.06024	0.05009
36	71	A	ELECTRON MICROSCOPY	Local ncs	0.08547	0.0501
36	72	A	ELECTRON MICROSCOPY	Local ncs	0.08547	0.0501
37	73	A	ELECTRON MICROSCOPY	Local ncs	0.07841	0.05009
37	74	A	ELECTRON MICROSCOPY	Local ncs	0.07841	0.05009
38	75	A	ELECTRON MICROSCOPY	Local ncs	0.01232	0.0501
38	76	A	ELECTRON MICROSCOPY	Local ncs	0.01232	0.0501
39	77	A	ELECTRON MICROSCOPY	Local ncs	0.0599	0.0501
39	78	A	ELECTRON MICROSCOPY	Local ncs	0.0599	0.0501
40	79	A	ELECTRON MICROSCOPY	Local ncs	0.08255	0.05009
40	80	A	ELECTRON MICROSCOPY	Local ncs	0.08255	0.05009
41	81	A	ELECTRON MICROSCOPY	Local ncs	0.05209	0.0501
41	82	A	ELECTRON MICROSCOPY	Local ncs	0.05209	0.0501
42	83	A	ELECTRON MICROSCOPY	Local ncs	0.08599	0.0501
42	84	A	ELECTRON MICROSCOPY	Local ncs	0.08599	0.0501
43	85	A	ELECTRON MICROSCOPY	Local ncs	0.07251	0.05009
43	86	A	ELECTRON MICROSCOPY	Local ncs	0.07251	0.05009
44	87	A	ELECTRON MICROSCOPY	Local ncs	0.06829	0.05009
44	88	A	ELECTRON MICROSCOPY	Local ncs	0.06829	0.05009
45	89	A	ELECTRON MICROSCOPY	Local ncs	0.09883	0.05008
45	90	A	ELECTRON MICROSCOPY	Local ncs	0.09883	0.05008
46	91	A	ELECTRON MICROSCOPY	Local ncs	0.08715	0.05009
46	92	A	ELECTRON MICROSCOPY	Local ncs	0.08715	0.05009
47	93	A	ELECTRON MICROSCOPY	Local ncs	0.0803	0.05009
47	94	A	ELECTRON MICROSCOPY	Local ncs	0.0803	0.05009
48	95	A	ELECTRON MICROSCOPY	Local ncs	0.0295	0.05009
48	96	A	ELECTRON MICROSCOPY	Local ncs	0.0295	0.05009
49	97	A	ELECTRON MICROSCOPY	Local ncs	0.07242	0.05009
49	98	A	ELECTRON MICROSCOPY	Local ncs	0.07242	0.05009
50	99	A	ELECTRON MICROSCOPY	Local ncs	0.06931	0.05009
50	100	A	ELECTRON MICROSCOPY	Local ncs	0.06931	0.05009
51	101	A	ELECTRON MICROSCOPY	Local ncs	0.09946	0.05009
51	102	A	ELECTRON MICROSCOPY	Local ncs	0.09946	0.05009
52	103	A	ELECTRON MICROSCOPY	Local ncs	0.08815	0.05009
52	104	A	ELECTRON MICROSCOPY	Local ncs	0.08815	0.05009
53	105	A	ELECTRON MICROSCOPY	Local ncs	0.06702	0.05009
53	106	A	ELECTRON MICROSCOPY	Local ncs	0.06702	0.05009
54	107	A	ELECTRON MICROSCOPY	Local ncs	0.08728	0.05009
54	108	A	ELECTRON MICROSCOPY	Local ncs	0.08728	0.05009
55	109	A	ELECTRON MICROSCOPY	Local ncs	0.08248	0.05009
55	110	A	ELECTRON MICROSCOPY	Local ncs	0.08248	0.05009
56	111	A	ELECTRON MICROSCOPY	Local ncs	0.0616	0.0501
56	112	A	ELECTRON MICROSCOPY	Local ncs	0.0616	0.0501
57	113	A	ELECTRON MICROSCOPY	Local ncs	0.03055	0.0501
57	114	A	ELECTRON MICROSCOPY	Local ncs	0.03055	0.0501
58	115	A	ELECTRON MICROSCOPY	Local ncs	0.0897	0.05009
58	116	A	ELECTRON MICROSCOPY	Local ncs	0.0897	0.05009
59	117	A	ELECTRON MICROSCOPY	Local ncs	0.05647	0.0501
59	118	A	ELECTRON MICROSCOPY	Local ncs	0.05647	0.0501
60	119	A	ELECTRON MICROSCOPY	Local ncs	0.08692	0.0501
60	120	A	ELECTRON MICROSCOPY	Local ncs	0.08692	0.0501
61	121	A	ELECTRON MICROSCOPY	Local ncs	0.07555	0.05009
61	122	A	ELECTRON MICROSCOPY	Local ncs	0.07555	0.05009
62	123	A	ELECTRON MICROSCOPY	Local ncs	0.10009	0.05009
62	124	A	ELECTRON MICROSCOPY	Local ncs	0.10009	0.05009
63	125	A	ELECTRON MICROSCOPY	Local ncs	0.07928	0.05009
63	126	A	ELECTRON MICROSCOPY	Local ncs	0.07928	0.05009
64	127	A	ELECTRON MICROSCOPY	Local ncs	0.08208	0.05009
64	128	A	ELECTRON MICROSCOPY	Local ncs	0.08208	0.05009
65	129	A	ELECTRON MICROSCOPY	Local ncs	0.07462	0.05009
65	130	A	ELECTRON MICROSCOPY	Local ncs	0.07462	0.05009
66	131	A	ELECTRON MICROSCOPY	Local ncs	0.00589	0.0501
66	132	A	ELECTRON MICROSCOPY	Local ncs	0.00589	0.0501
67	133	A	ELECTRON MICROSCOPY	Local ncs	0.07536	0.05009
67	134	A	ELECTRON MICROSCOPY	Local ncs	0.07536	0.05009
68	135	A	ELECTRON MICROSCOPY	Local ncs	0.1014	0.05009
68	136	A	ELECTRON MICROSCOPY	Local ncs	0.1014	0.05009
69	137	A	ELECTRON MICROSCOPY	Local ncs	0.09023	0.05009
69	138	A	ELECTRON MICROSCOPY	Local ncs	0.09023	0.05009
70	139	A	ELECTRON MICROSCOPY	Local ncs	0.09477	0.05009
70	140	A	ELECTRON MICROSCOPY	Local ncs	0.09477	0.05009
71	141	A	ELECTRON MICROSCOPY	Local ncs	0.09589	0.05009
71	142	A	ELECTRON MICROSCOPY	Local ncs	0.09589	0.05009
72	143	A	ELECTRON MICROSCOPY	Local ncs	0.07723	0.05009
72	144	A	ELECTRON MICROSCOPY	Local ncs	0.07723	0.05009
73	145	A	ELECTRON MICROSCOPY	Local ncs	0.08548	0.05009
73	146	A	ELECTRON MICROSCOPY	Local ncs	0.08548	0.05009
74	147	A	ELECTRON MICROSCOPY	Local ncs	0.05248	0.0501
74	148	A	ELECTRON MICROSCOPY	Local ncs	0.05248	0.0501
75	149	A	ELECTRON MICROSCOPY	Local ncs	0.08255	0.05009
75	150	A	ELECTRON MICROSCOPY	Local ncs	0.08255	0.05009
76	151	A	ELECTRON MICROSCOPY	Local ncs	0.09529	0.05009
76	152	A	ELECTRON MICROSCOPY	Local ncs	0.09529	0.05009
77	153	A	ELECTRON MICROSCOPY	Local ncs	0.10236	0.05008
77	154	A	ELECTRON MICROSCOPY	Local ncs	0.10236	0.05008
78	155	A	ELECTRON MICROSCOPY	Local ncs	0.08765	0.05009
78	156	A	ELECTRON MICROSCOPY	Local ncs	0.08765	0.05009
79	157	A	ELECTRON MICROSCOPY	Local ncs	0.0888	0.05009
79	158	A	ELECTRON MICROSCOPY	Local ncs	0.0888	0.05009
80	159	A	ELECTRON MICROSCOPY	Local ncs	0.07353	0.05009
80	160	A	ELECTRON MICROSCOPY	Local ncs	0.07353	0.05009
81	161	A	ELECTRON MICROSCOPY	Local ncs	0.07566	0.05009
81	162	A	ELECTRON MICROSCOPY	Local ncs	0.07566	0.05009
82	163	A	ELECTRON MICROSCOPY	Local ncs	0.00569	0.0501
82	164	A	ELECTRON MICROSCOPY	Local ncs	0.00569	0.0501
83	165	A	ELECTRON MICROSCOPY	Local ncs	0.09912	0.05009
83	166	A	ELECTRON MICROSCOPY	Local ncs	0.09912	0.05009
84	167	A	ELECTRON MICROSCOPY	Local ncs	0.09133	0.05009
84	168	A	ELECTRON MICROSCOPY	Local ncs	0.09133	0.05009
85	169	A	ELECTRON MICROSCOPY	Local ncs	0.06327	0.0501
85	170	A	ELECTRON MICROSCOPY	Local ncs	0.06327	0.0501
86	171	A	ELECTRON MICROSCOPY	Local ncs	0.06029	0.05009
86	172	A	ELECTRON MICROSCOPY	Local ncs	0.06029	0.05009
87	173	A	ELECTRON MICROSCOPY	Local ncs	0.06413	0.05009
87	174	A	ELECTRON MICROSCOPY	Local ncs	0.06413	0.05009
88	175	A	ELECTRON MICROSCOPY	Local ncs	0.09329	0.05009
88	176	A	ELECTRON MICROSCOPY	Local ncs	0.09329	0.05009
89	177	A	ELECTRON MICROSCOPY	Local ncs	0.04019	0.0501
89	178	A	ELECTRON MICROSCOPY	Local ncs	0.04019	0.0501
90	179	A	ELECTRON MICROSCOPY	Local ncs	0.09087	0.05009
90	180	A	ELECTRON MICROSCOPY	Local ncs	0.09087	0.05009
91	181	A	ELECTRON MICROSCOPY	Local ncs	0.10596	0.05008
91	182	A	ELECTRON MICROSCOPY	Local ncs	0.10596	0.05008
92	183	A	ELECTRON MICROSCOPY	Local ncs	0.09407	0.05009
92	184	A	ELECTRON MICROSCOPY	Local ncs	0.09407	0.05009
93	185	A	ELECTRON MICROSCOPY	Local ncs	0.10121	0.05008
93	186	A	ELECTRON MICROSCOPY	Local ncs	0.10121	0.05008
94	187	A	ELECTRON MICROSCOPY	Local ncs	0.09949	0.05009
94	188	A	ELECTRON MICROSCOPY	Local ncs	0.09949	0.05009
95	189	A	ELECTRON MICROSCOPY	Local ncs	0.10226	0.05009
95	190	A	ELECTRON MICROSCOPY	Local ncs	0.10226	0.05009
96	191	A	ELECTRON MICROSCOPY	Local ncs	0.07038	0.05009
96	192	A	ELECTRON MICROSCOPY	Local ncs	0.07038	0.05009
97	193	A	ELECTRON MICROSCOPY	Local ncs	0.09922	0.05009
97	194	A	ELECTRON MICROSCOPY	Local ncs	0.09922	0.05009
98	195	A	ELECTRON MICROSCOPY	Local ncs	0.08355	0.0501
98	196	A	ELECTRON MICROSCOPY	Local ncs	0.08355	0.0501
99	197	A	ELECTRON MICROSCOPY	Local ncs	0.08589	0.05009
99	198	A	ELECTRON MICROSCOPY	Local ncs	0.08589	0.05009
100	199	A	ELECTRON MICROSCOPY	Local ncs	0.09837	0.05009
100	200	A	ELECTRON MICROSCOPY	Local ncs	0.09837	0.05009
101	201	A	ELECTRON MICROSCOPY	Local ncs	0.08725	0.05009
101	202	A	ELECTRON MICROSCOPY	Local ncs	0.08725	0.05009
102	203	A	ELECTRON MICROSCOPY	Local ncs	0.0204	0.0501
102	204	A	ELECTRON MICROSCOPY	Local ncs	0.0204	0.0501
103	205	A	ELECTRON MICROSCOPY	Local ncs	0.09308	0.05009
103	206	A	ELECTRON MICROSCOPY	Local ncs	0.09308	0.05009
104	207	A	ELECTRON MICROSCOPY	Local ncs	0.08844	0.05008
104	208	A	ELECTRON MICROSCOPY	Local ncs	0.08844	0.05008
105	209	A	ELECTRON MICROSCOPY	Local ncs	0.07944	0.05009
105	210	A	ELECTRON MICROSCOPY	Local ncs	0.07944	0.05009
106	211	A	ELECTRON MICROSCOPY	Local ncs	0.08738	0.05009
106	212	A	ELECTRON MICROSCOPY	Local ncs	0.08738	0.05009
107	213	A	ELECTRON MICROSCOPY	Local ncs	0.11076	0.05008
107	214	A	ELECTRON MICROSCOPY	Local ncs	0.11076	0.05008
108	215	A	ELECTRON MICROSCOPY	Local ncs	0.07786	0.05009
108	216	A	ELECTRON MICROSCOPY	Local ncs	0.07786	0.05009
109	217	A	ELECTRON MICROSCOPY	Local ncs	0.08013	0.05009
109	218	A	ELECTRON MICROSCOPY	Local ncs	0.08013	0.05009
110	219	A	ELECTRON MICROSCOPY	Local ncs	0.08325	0.05009
110	220	A	ELECTRON MICROSCOPY	Local ncs	0.08325	0.05009
111	221	A	ELECTRON MICROSCOPY	Local ncs	0.07417	0.05009
111	222	A	ELECTRON MICROSCOPY	Local ncs	0.07417	0.05009
112	223	A	ELECTRON MICROSCOPY	Local ncs	0.09893	0.05009
112	224	A	ELECTRON MICROSCOPY	Local ncs	0.09893	0.05009
113	225	A	ELECTRON MICROSCOPY	Local ncs	0.08129	0.05009
113	226	A	ELECTRON MICROSCOPY	Local ncs	0.08129	0.05009
114	227	A	ELECTRON MICROSCOPY	Local ncs	0.08192	0.05009
114	228	A	ELECTRON MICROSCOPY	Local ncs	0.08192	0.05009
115	229	A	ELECTRON MICROSCOPY	Local ncs	0.08872	0.05009
115	230	A	ELECTRON MICROSCOPY	Local ncs	0.08872	0.05009
116	231	A	ELECTRON MICROSCOPY	Local ncs	0.09182	0.05009
116	232	A	ELECTRON MICROSCOPY	Local ncs	0.09182	0.05009
117	233	A	ELECTRON MICROSCOPY	Local ncs	0.05855	0.0501
117	234	A	ELECTRON MICROSCOPY	Local ncs	0.05855	0.0501
118	235	A	ELECTRON MICROSCOPY	Local ncs	0.0811	0.0501
118	236	A	ELECTRON MICROSCOPY	Local ncs	0.0811	0.0501
119	237	A	ELECTRON MICROSCOPY	Local ncs	0.0518	0.0501
119	238	A	ELECTRON MICROSCOPY	Local ncs	0.0518	0.0501
120	239	A	ELECTRON MICROSCOPY	Local ncs	0.08364	0.0501
120	240	A	ELECTRON MICROSCOPY	Local ncs	0.08364	0.0501
121	241	A	ELECTRON MICROSCOPY	Local ncs	0.07461	0.05009
121	242	A	ELECTRON MICROSCOPY	Local ncs	0.07461	0.05009
122	243	A	ELECTRON MICROSCOPY	Local ncs	0.07364	0.05009
122	244	A	ELECTRON MICROSCOPY	Local ncs	0.07364	0.05009
123	245	A	ELECTRON MICROSCOPY	Local ncs	0.09957	0.05008
123	246	A	ELECTRON MICROSCOPY	Local ncs	0.09957	0.05008
124	247	A	ELECTRON MICROSCOPY	Local ncs	0.08918	0.0501
124	248	A	ELECTRON MICROSCOPY	Local ncs	0.08918	0.0501
125	249	A	ELECTRON MICROSCOPY	Local ncs	0.0538	0.0501
125	250	A	ELECTRON MICROSCOPY	Local ncs	0.0538	0.0501
126	251	A	ELECTRON MICROSCOPY	Local ncs	0.08525	0.0501
126	252	A	ELECTRON MICROSCOPY	Local ncs	0.08525	0.0501
127	253	A	ELECTRON MICROSCOPY	Local ncs	0.07538	0.05009
127	254	A	ELECTRON MICROSCOPY	Local ncs	0.07538	0.05009
128	255	A	ELECTRON MICROSCOPY	Local ncs	0.10008	0.05009
128	256	A	ELECTRON MICROSCOPY	Local ncs	0.10008	0.05009
129	257	A	ELECTRON MICROSCOPY	Local ncs	0.08472	0.05009
129	258	A	ELECTRON MICROSCOPY	Local ncs	0.08472	0.05009
130	259	A	ELECTRON MICROSCOPY	Local ncs	0.09935	0.05009
130	260	A	ELECTRON MICROSCOPY	Local ncs	0.09935	0.05009
131	261	A	ELECTRON MICROSCOPY	Local ncs	0.09883	0.05009
131	262	A	ELECTRON MICROSCOPY	Local ncs	0.09883	0.05009
132	263	A	ELECTRON MICROSCOPY	Local ncs	0.08724	0.05009
132	264	A	ELECTRON MICROSCOPY	Local ncs	0.08724	0.05009

LS精密化 シェル

Refine-ID: ELECTRON MICROSCOPY / Num. reflection R_free: _ / Total num. of bins used: 20 / % reflection obs: 100 %

解像度 (Å)	Rfactor Rwork	Num. reflection Rwork	Rfactor all	Num. reflection all	Fsc work	WRfactor Rwork
3.15-3.232	0.557	15132	0.557	15132	0.482	0.557
3.232-3.32	0.462	14538	0.462	14538	0.575	0.462
3.32-3.417	0.44	14201	0.44	14201	0.631	0.44
3.417-3.522	0.429	13863	0.429	13863	0.659	0.429
3.522-3.637	0.414	13419	0.414	13419	0.699	0.414
3.637-3.765	0.397	12938	0.397	12938	0.74	0.397
3.765-3.907	0.383	12448	0.383	12448	0.773	0.383
3.907-4.066	0.368	12080	0.368	12080	0.793	0.368
4.066-4.247	0.353	11457	0.353	11457	0.818	0.353
4.247-4.454	0.342	11048	0.342	11048	0.831	0.342
4.454-4.695	0.323	10488	0.323	10488	0.849	0.323
4.695-4.979	0.316	9869	0.316	9869	0.853	0.316
4.979-5.322	0.322	9374	0.322	9374	0.855	0.322
5.322-5.748	0.356	8665	0.356	8665	0.854	0.356
5.748-6.296	0.383	7936	0.383	7936	0.849	0.383
6.296-7.038	0.344	7243	0.344	7243	0.872	0.344
7.038-8.124	0.283	6354	0.283	6354	0.889	0.283
8.124-9.943	0.245	5362	0.245	5362	0.927	0.245
9.943-14.032	0.238	4182	0.238	4182	0.949	0.238
14.032-154.823	0.184	2269	0.184	2269	0.985	0.184