PDB-9v7h: Phycobilisome rod R2 from Gloeobacter violaceus PCC 7421

Basic information

• Entry: Database: PDB / ID: 9v7h
• Title: Phycobilisome rod R2 from Gloeobacter violaceus PCC 7421

Components

• (Phycocyanin-associated rod linker ...) x 2
• Glr1262 protein
• Phycocyanin alpha chain
• Phycocyanin beta chain

• Keywords: PHOTOSYNTHESIS / Phycobilisome

Function / homology

Function:

phycobilisome / plasma membrane-derived thylakoid membrane / photosynthesis

Domain/homology:

Phycobilisome linker domain / Phycobilisome linker domain superfamily / Phycobilisome Linker polypeptide / Phycobilisome (PBS) linker domain profile. / Phycobilisome, alpha/beta subunit / Phycobilisome, alpha/beta subunit superfamily / Phycobilisome protein / Globin-like superfamily

Component:

PHYCOCYANOBILIN / Phycocyanin beta chain / Phycocyanin alpha chain / Glr1262 protein

• Biological species: Gloeobacter violaceus PCC 7421 (bacteria)
• Method: ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.95 Å
• Authors: Burtseva, A.D. / Baymukhametov, T.N. / Slonimskiy, Y.B. / Popov, V.O. / Sluchanko, N.N. / Boyko, K.M.

Funding support

Russian Federation, 1items

Organization	Grant number	Country
Russian Science Foundation	23-74-00062	Russian Federation

• Citation: Journal: Sci Adv / Year: 2025; Title: Structure and quenching of a bundle-shaped phycobilisome.; Authors: Anna D Burtseva / Yury B Slonimskiy / Timur N Baymukhametov / Maria A Sinetova / Daniil A Gvozdev / Georgy V Tsoraev / Dmitry A Cherepanov / Eugene G Maksimov / Vladimir O Popov / Konstantin M Boyko / Nikolai N Sluchanko / ; Abstract: Cyanobacteria use soluble antenna megacomplexes, phycobilisomes (PBSs), to maximize light-harvesting efficiency and small photoswitchable orange carotenoid proteins (OCPs) to down-regulate PBSs in high light. Among known PBS morphologies, the one from the basal cyanobacterial genus still lacks detailed structural characterization. Here, we reconstructed a cryo-electron microscopy structure of the >10-megadalton PBS, with diverging, conformationally mobile bundles of rods composed of stacked phycoerythrin and phycocyanin hexamers, stemming from a pentacylindrical allophycocyanin core belted by auxiliary phycocyanin hexamers. We show how two -specific multidomain linker proteins, Glr1262 and Glr2806, maintain this bundle-shaped architecture and reveal its differential regulation via nonphotochemical quenching by two OCP types of that recognize separate binding sites within the allophycocyanin core, including lateral cylinders absent in tricylindrical cores.

History

Deposition	May 28, 2025	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Sep 24, 2025	Provider: repository / Type: Initial release
Revision 1.0	Sep 24, 2025	Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0	Sep 24, 2025	Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0	Sep 24, 2025	Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0	Sep 24, 2025	Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0	Sep 24, 2025	Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release
Revision 1.1	Oct 29, 2025	Group: Data collection / Database references / Category: citation / citation_author / em_admin Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _em_admin.last_update

Assembly

Deposited unit

A: Phycocyanin-associated rod linker protein

B: Phycocyanin beta chain

C: Phycocyanin alpha chain

D: Phycocyanin beta chain

E: Phycocyanin alpha chain

F: Phycocyanin beta chain

G: Phycocyanin alpha chain

H: Phycocyanin beta chain

I: Phycocyanin alpha chain

J: Phycocyanin beta chain

K: Phycocyanin alpha chain

L: Phycocyanin beta chain

M: Phycocyanin-associated rod linker protein

N: Phycocyanin alpha chain

O: Phycocyanin beta chain

P: Phycocyanin alpha chain

Q: Phycocyanin beta chain

R: Phycocyanin alpha chain

S: Phycocyanin beta chain

T: Phycocyanin alpha chain

U: Phycocyanin beta chain

V: Phycocyanin alpha chain

W: Phycocyanin beta chain

X: Phycocyanin alpha chain

Y: Phycocyanin beta chain

Z: Glr1262 protein

a: Phycocyanin alpha chain

hetero molecules

Summary:

• 609 kDa, 27 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	609,225	63
Polymers	588,032	27
Non-polymers	21,193	36
Water	0	0

1

Summary:

• Idetical with deposited unit
• defined by author
• Evidence: electron microscopy, not applicable

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555		1

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	A
2	1	A
3	2	A
4	2	A
5	3	A
6	3	A
7	4	A
8	4	A
9	5	A
10	5	A
11	6	A
12	6	A
13	7	A
14	7	A
15	8	A
16	8	A
17	9	A
18	9	A
19	10	A
20	10	A
21	11	A
22	11	A
23	12	A
24	12	A
25	13	A
26	13	A
27	14	A
28	14	A
29	15	A
30	15	A
31	16	A
32	16	A
33	17	A
34	17	A
35	18	A
36	18	A
37	19	A
38	19	A
39	20	A
40	20	A
41	21	A
42	21	A
43	22	A
44	22	A
45	23	A
46	23	A
47	24	A
48	24	A
49	25	A
50	25	A
51	26	A
52	26	A
53	27	A
54	27	A
55	28	A
56	28	A
57	29	A
58	29	A
59	30	A
60	30	A
61	31	A
62	31	A
63	32	A
64	32	A
65	33	A
66	33	A
67	34	A
68	34	A
69	35	A
70	35	A
71	36	A
72	36	A
73	37	A
74	37	A
75	38	A
76	38	A
77	39	A
78	39	A
79	40	A
80	40	A
81	41	A
82	41	A
83	42	A
84	42	A
85	43	A
86	43	A
87	44	A
88	44	A
89	45	A
90	45	A
91	46	A
92	46	A
93	47	A
94	47	A
95	48	A
96	48	A
97	49	A
98	49	A
99	50	A
100	50	A
101	51	A
102	51	A
103	52	A
104	52	A
105	53	A
106	53	A
107	54	A
108	54	A
109	55	A
110	55	A
111	56	A
112	56	A
113	57	A
114	57	A
115	58	A
116	58	A
117	59	A
118	59	A
119	60	A
120	60	A
121	61	A
122	61	A
123	62	A
124	62	A
125	63	A
126	63	A
127	64	A
128	64	A
129	65	A
130	65	A
131	66	A
132	66	A
133	67	A
134	67	A
135	68	A
136	68	A
137	69	A
138	69	A
139	70	A
140	70	A
141	71	A
142	71	A
143	72	A
144	72	A
145	73	A
146	73	A
147	74	A
148	74	A
149	75	A
150	75	A
151	76	A
152	76	A
153	77	A
154	77	A
155	78	A
156	78	A
157	79	A
158	79	A
159	80	A
160	80	A
161	81	A
162	81	A
163	82	A
164	82	A
165	83	A
166	83	A
167	84	A
168	84	A
169	85	A
170	85	A
171	86	A
172	86	A
173	87	A
174	87	A
175	88	A
176	88	A
177	89	A
178	89	A
179	90	A
180	90	A
181	91	A
182	91	A
183	92	A
184	92	A
185	93	A
186	93	A
187	94	A
188	94	A
189	95	A
190	95	A
191	96	A
192	96	A
193	97	A
194	97	A
195	98	A
196	98	A
197	99	A
198	99	A
199	100	A
200	100	A
201	101	A
202	101	A
203	102	A
204	102	A
205	103	A
206	103	A
207	104	A
208	104	A
209	105	A
210	105	A
211	106	A
212	106	A
213	107	A
214	107	A
215	108	A
216	108	A
217	109	A
218	109	A
219	110	A
220	110	A
221	111	A
222	111	A
223	112	A
224	112	A
225	113	A
226	113	A
227	114	A
228	114	A
229	115	A
230	115	A
231	116	A
232	116	A
233	117	A
234	117	A
235	118	A
236	118	A
237	119	A
238	119	A
239	120	A
240	120	A
241	121	A
242	121	A
243	122	A
244	122	A
245	123	A
246	123	A
247	124	A
248	124	A
249	125	A
250	125	A
251	126	A
252	126	A
253	127	A
254	127	A
255	128	A
256	128	A
257	129	A
258	129	A
259	130	A
260	130	A
261	131	A
262	131	A
263	132	A
264	132	A

NCS ensembles :

ID	Details
1	Local NCS retraints between domains: 1 2
2	Local NCS retraints between domains: 3 4
3	Local NCS retraints between domains: 5 6
4	Local NCS retraints between domains: 7 8
5	Local NCS retraints between domains: 9 10
6	Local NCS retraints between domains: 11 12
7	Local NCS retraints between domains: 13 14
8	Local NCS retraints between domains: 15 16
9	Local NCS retraints between domains: 17 18
10	Local NCS retraints between domains: 19 20
11	Local NCS retraints between domains: 21 22
12	Local NCS retraints between domains: 23 24
13	Local NCS retraints between domains: 25 26
14	Local NCS retraints between domains: 27 28
15	Local NCS retraints between domains: 29 30
16	Local NCS retraints between domains: 31 32
17	Local NCS retraints between domains: 33 34
18	Local NCS retraints between domains: 35 36
19	Local NCS retraints between domains: 37 38
20	Local NCS retraints between domains: 39 40
21	Local NCS retraints between domains: 41 42
22	Local NCS retraints between domains: 43 44
23	Local NCS retraints between domains: 45 46
24	Local NCS retraints between domains: 47 48
25	Local NCS retraints between domains: 49 50
26	Local NCS retraints between domains: 51 52
27	Local NCS retraints between domains: 53 54
28	Local NCS retraints between domains: 55 56
29	Local NCS retraints between domains: 57 58
30	Local NCS retraints between domains: 59 60
31	Local NCS retraints between domains: 61 62
32	Local NCS retraints between domains: 63 64
33	Local NCS retraints between domains: 65 66
34	Local NCS retraints between domains: 67 68
35	Local NCS retraints between domains: 69 70
36	Local NCS retraints between domains: 71 72
37	Local NCS retraints between domains: 73 74
38	Local NCS retraints between domains: 75 76
39	Local NCS retraints between domains: 77 78
40	Local NCS retraints between domains: 79 80
41	Local NCS retraints between domains: 81 82
42	Local NCS retraints between domains: 83 84
43	Local NCS retraints between domains: 85 86
44	Local NCS retraints between domains: 87 88
45	Local NCS retraints between domains: 89 90
46	Local NCS retraints between domains: 91 92
47	Local NCS retraints between domains: 93 94
48	Local NCS retraints between domains: 95 96
49	Local NCS retraints between domains: 97 98
50	Local NCS retraints between domains: 99 100
51	Local NCS retraints between domains: 101 102
52	Local NCS retraints between domains: 103 104
53	Local NCS retraints between domains: 105 106
54	Local NCS retraints between domains: 107 108
55	Local NCS retraints between domains: 109 110
56	Local NCS retraints between domains: 111 112
57	Local NCS retraints between domains: 113 114
58	Local NCS retraints between domains: 115 116
59	Local NCS retraints between domains: 117 118
60	Local NCS retraints between domains: 119 120
61	Local NCS retraints between domains: 121 122
62	Local NCS retraints between domains: 123 124
63	Local NCS retraints between domains: 125 126
64	Local NCS retraints between domains: 127 128
65	Local NCS retraints between domains: 129 130
66	Local NCS retraints between domains: 131 132
67	Local NCS retraints between domains: 133 134
68	Local NCS retraints between domains: 135 136
69	Local NCS retraints between domains: 137 138
70	Local NCS retraints between domains: 139 140
71	Local NCS retraints between domains: 141 142
72	Local NCS retraints between domains: 143 144
73	Local NCS retraints between domains: 145 146
74	Local NCS retraints between domains: 147 148
75	Local NCS retraints between domains: 149 150
76	Local NCS retraints between domains: 151 152
77	Local NCS retraints between domains: 153 154
78	Local NCS retraints between domains: 155 156
79	Local NCS retraints between domains: 157 158
80	Local NCS retraints between domains: 159 160
81	Local NCS retraints between domains: 161 162
82	Local NCS retraints between domains: 163 164
83	Local NCS retraints between domains: 165 166
84	Local NCS retraints between domains: 167 168
85	Local NCS retraints between domains: 169 170
86	Local NCS retraints between domains: 171 172
87	Local NCS retraints between domains: 173 174
88	Local NCS retraints between domains: 175 176
89	Local NCS retraints between domains: 177 178
90	Local NCS retraints between domains: 179 180
91	Local NCS retraints between domains: 181 182
92	Local NCS retraints between domains: 183 184
93	Local NCS retraints between domains: 185 186
94	Local NCS retraints between domains: 187 188
95	Local NCS retraints between domains: 189 190
96	Local NCS retraints between domains: 191 192
97	Local NCS retraints between domains: 193 194
98	Local NCS retraints between domains: 195 196
99	Local NCS retraints between domains: 197 198
100	Local NCS retraints between domains: 199 200
101	Local NCS retraints between domains: 201 202
102	Local NCS retraints between domains: 203 204
103	Local NCS retraints between domains: 205 206
104	Local NCS retraints between domains: 207 208
105	Local NCS retraints between domains: 209 210
106	Local NCS retraints between domains: 211 212
107	Local NCS retraints between domains: 213 214
108	Local NCS retraints between domains: 215 216
109	Local NCS retraints between domains: 217 218
110	Local NCS retraints between domains: 219 220
111	Local NCS retraints between domains: 221 222
112	Local NCS retraints between domains: 223 224
113	Local NCS retraints between domains: 225 226
114	Local NCS retraints between domains: 227 228
115	Local NCS retraints between domains: 229 230
116	Local NCS retraints between domains: 231 232
117	Local NCS retraints between domains: 233 234
118	Local NCS retraints between domains: 235 236
119	Local NCS retraints between domains: 237 238
120	Local NCS retraints between domains: 239 240
121	Local NCS retraints between domains: 241 242
122	Local NCS retraints between domains: 243 244
123	Local NCS retraints between domains: 245 246
124	Local NCS retraints between domains: 247 248
125	Local NCS retraints between domains: 249 250
126	Local NCS retraints between domains: 251 252
127	Local NCS retraints between domains: 253 254
128	Local NCS retraints between domains: 255 256
129	Local NCS retraints between domains: 257 258
130	Local NCS retraints between domains: 259 260
131	Local NCS retraints between domains: 261 262
132	Local NCS retraints between domains: 263 264

Components

Phycocyanin-associated rod linker ... , 2 types, 2 molecules A M

#1: Protein

A Phycocyanin-associated rod linker protein

Details:

Mass: 31085.066 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Gloeobacter violaceus PCC 7421 (bacteria)

#4: Protein

M Phycocyanin-associated rod linker protein

Details:

Mass: 30913.822 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Gloeobacter violaceus PCC 7421 (bacteria)

Protein , 3 types, 25 molecules B D F H J L O Q S U W Y C E G I K N P R T V X a Z

#2: Protein

B D F H J L O Q S U W Y
Phycocyanin beta chain

Details:

Mass: 18478.953 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Gloeobacter violaceus PCC 7421 (bacteria) / References: UniProt: Q7M7C7

#3: Protein

C E G I K N P R T V X a
Phycocyanin alpha chain

Details:

Mass: 17679.852 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Gloeobacter violaceus PCC 7421 (bacteria) / References: UniProt: Q7M7F7

#5: Protein

Z Glr1262 protein

Details:

Mass: 92127.602 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Gloeobacter violaceus PCC 7421 (bacteria) / References: UniProt: Q7NL64

Non-polymers , 1 types, 36 molecules

#6: Chemical

B-201 B-202 C-201 D-201 D-202 E-201 F-201 F-202 G-201 H-201 H-202 I-201 J-201 J-202 K-201 L-201 L-202 N-201 O-201 O-202 ...
ChemComp-CYC / PHYCOCYANOBILIN

Details:

Mass: 588.694 Da / Num. of mol.: 36 / Source method: obtained synthetically / Formula: C₃₃H₄₀N₄O₆ / Feature type: SUBJECT OF INVESTIGATION

Details

• Has ligand of interest: Y
• Has protein modification: Y

Experimental details

Experiment

• Experiment: Method: ELECTRON MICROSCOPY
• EM experiment: Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

Sample preparation

• Component: Name: Bundle-shaped phycobilisome / Type: COMPLEX / Entity ID: #1-#5 / Source: NATURAL
• Source (natural): Organism: Gloeobacter violaceus PCC 7421 (bacteria)
• Buffer solution: pH: 7
• Buffer component: Conc.: 50 mM / Name: Tris(hydroxymethyl)aminomethane hydrochloride / Formula: Tris-HCl
• Specimen: Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
• Vitrification: Cryogen name: ETHANE

Electron microscopy imaging

• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company
• Microscopy: Model: TFS KRIOS / Details: Preliminary grid screening was performed manually.
• Electron gun: Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
• Electron lens: Mode: BRIGHT FIELD / Nominal magnification: 85000 X / Nominal defocus max: 1600 nm / Nominal defocus min: 600 nm / Cs: 0.01 mm / C2 aperture diameter: 100 µm / Alignment procedure: ZEMLIN TABLEAU
• Specimen holder: Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
• Image recording: Average exposure time: 3.9 sec. / Electron dose: 66 e/Ų / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)
• EM imaging optics: Energyfilter name: GIF Bioquantum / Energyfilter slit width: 20 eV; Spherical aberration corrector: Microscope was modified with a Cs corrector (CEOS GmbH, Germany).
• Image scans: Width: 5760 / Height: 4092

Processing

EM software

ID	Name	Version	Category
2	SerialEM	4.04	image acquisition
4	Warp	1.0.9	CTF correction
11	cryoSPARC	4.7	final Euler assignment
13	cryoSPARC	4.7	3D reconstruction

• CTF correction: Type: NONE
• Symmetry: Point symmetry: C₁ (asymmetric)
• 3D reconstruction: Resolution: 2.95 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 207644 / Symmetry type: POINT

Refinement

Resolution: 2.95→2.95 Å / Cor.coef. F_o:F_c: 0.948 / WR_factor R_work: 0.306 / SU B: 29.793 / SU ML: 0.499 / Average fsc free: 0 / Average fsc overall: 0.8174 / Average fsc work: 0.8174 / ESU R: 0.69
Details: Hydrogens have been added in their riding positions

	Rfactor	Num. reflection	% reflection
Rwork	0.3064	217183	-
all	0.306	-	-
Rfree	-	-	0 %
obs	-	-	100 %

• Solvent computation: Solvent model: NONE
• Displacement parameters: B_iso mean: 45.623 Ų

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
ELECTRON MICROSCOPY	r_bond_refined_d	0.008	0.012	37150
ELECTRON MICROSCOPY	r_bond_other_d	0.001	0.016	35230
ELECTRON MICROSCOPY	r_angle_refined_deg	1.737	1.814	50558
ELECTRON MICROSCOPY	r_angle_other_deg	0.666	1.748	80604
ELECTRON MICROSCOPY	r_dihedral_angle_1_deg	6.148	5	4548
ELECTRON MICROSCOPY	r_dihedral_angle_2_deg	15.698	8.529	918
ELECTRON MICROSCOPY	r_dihedral_angle_3_deg	16.86	10	5925
ELECTRON MICROSCOPY	r_dihedral_angle_6_deg	13.903	10	1608
ELECTRON MICROSCOPY	r_chiral_restr	0.07	0.2	5605
ELECTRON MICROSCOPY	r_gen_planes_refined	0.007	0.02	45952
ELECTRON MICROSCOPY	r_gen_planes_other	0.004	0.02	8594
ELECTRON MICROSCOPY	r_nbd_refined	0.318	0.2	11785
ELECTRON MICROSCOPY	r_symmetry_nbd_other	0.223	0.2	34891
ELECTRON MICROSCOPY	r_nbtor_refined	0.208	0.2	20133
ELECTRON MICROSCOPY	r_symmetry_nbtor_other	0.084	0.2	21017
ELECTRON MICROSCOPY	r_xyhbond_nbd_refined	0.178	0.2	1214
ELECTRON MICROSCOPY	r_symmetry_xyhbond_nbd_other	0.152	0.2	38
ELECTRON MICROSCOPY	r_mcbond_it	1.516	4.549	18273
ELECTRON MICROSCOPY	r_mcbond_other	1.516	4.549	18273
ELECTRON MICROSCOPY	r_mcangle_it	2.514	8.156	22794
ELECTRON MICROSCOPY	r_mcangle_other	2.514	8.156	22795
ELECTRON MICROSCOPY	r_scbond_it	1.54	4.649	18877
ELECTRON MICROSCOPY	r_scbond_other	1.54	4.648	18876
ELECTRON MICROSCOPY	r_scangle_it	2.758	8.468	27764
ELECTRON MICROSCOPY	r_scangle_other	2.758	8.468	27765
ELECTRON MICROSCOPY	r_lrange_it	4.476	41.25	47235
ELECTRON MICROSCOPY	r_lrange_other	4.476	41.25	47236
ELECTRON MICROSCOPY	r_ncsr_local_group_1	0.096	0.05	5350
ELECTRON MICROSCOPY	r_ncsr_local_group_2	0.082	0.05	5353
ELECTRON MICROSCOPY	r_ncsr_local_group_3	0.073	0.05	5441
ELECTRON MICROSCOPY	r_ncsr_local_group_4	0.082	0.05	5385
ELECTRON MICROSCOPY	r_ncsr_local_group_5	0.104	0.05	5312
ELECTRON MICROSCOPY	r_ncsr_local_group_6	0.045	0.05	5515
ELECTRON MICROSCOPY	r_ncsr_local_group_7	0.086	0.05	5381
ELECTRON MICROSCOPY	r_ncsr_local_group_8	0.08	0.05	5378
ELECTRON MICROSCOPY	r_ncsr_local_group_9	0.073	0.05	5437
ELECTRON MICROSCOPY	r_ncsr_local_group_10	0.083	0.05	5390
ELECTRON MICROSCOPY	r_ncsr_local_group_11	0.099	0.05	5304
ELECTRON MICROSCOPY	r_ncsr_local_group_12	0.057	0.05	5328
ELECTRON MICROSCOPY	r_ncsr_local_group_13	0.058	0.05	5354
ELECTRON MICROSCOPY	r_ncsr_local_group_14	0.075	0.05	5330
ELECTRON MICROSCOPY	r_ncsr_local_group_15	0.052	0.05	5336
ELECTRON MICROSCOPY	r_ncsr_local_group_16	0.088	0.05	5300
ELECTRON MICROSCOPY	r_ncsr_local_group_17	0.006	0.05	5435
ELECTRON MICROSCOPY	r_ncsr_local_group_18	0.058	0.05	5327
ELECTRON MICROSCOPY	r_ncsr_local_group_19	0.058	0.05	5355
ELECTRON MICROSCOPY	r_ncsr_local_group_20	0.074	0.05	5331
ELECTRON MICROSCOPY	r_ncsr_local_group_21	0.052	0.05	5333
ELECTRON MICROSCOPY	r_ncsr_local_group_22	0.088	0.05	5294
ELECTRON MICROSCOPY	r_ncsr_local_group_23	0.092	0.05	5316
ELECTRON MICROSCOPY	r_ncsr_local_group_24	0.09	0.05	5397
ELECTRON MICROSCOPY	r_ncsr_local_group_25	0.095	0.05	5350
ELECTRON MICROSCOPY	r_ncsr_local_group_26	0.103	0.05	5368
ELECTRON MICROSCOPY	r_ncsr_local_group_27	0.102	0.05	5335
ELECTRON MICROSCOPY	r_ncsr_local_group_28	0.053	0.05	5560
ELECTRON MICROSCOPY	r_ncsr_local_group_29	0.092	0.05	5343
ELECTRON MICROSCOPY	r_ncsr_local_group_30	0.09	0.05	5399
ELECTRON MICROSCOPY	r_ncsr_local_group_31	0.096	0.05	5358
ELECTRON MICROSCOPY	r_ncsr_local_group_32	0.097	0.05	5373
ELECTRON MICROSCOPY	r_ncsr_local_group_33	0.054	0.05	5349
ELECTRON MICROSCOPY	r_ncsr_local_group_34	0.07	0.05	5310
ELECTRON MICROSCOPY	r_ncsr_local_group_35	0.046	0.05	5363
ELECTRON MICROSCOPY	r_ncsr_local_group_36	0.083	0.05	5291
ELECTRON MICROSCOPY	r_ncsr_local_group_37	0.058	0.05	5317
ELECTRON MICROSCOPY	r_ncsr_local_group_38	0.005	0.05	5427
ELECTRON MICROSCOPY	r_ncsr_local_group_39	0.055	0.05	5352
ELECTRON MICROSCOPY	r_ncsr_local_group_40	0.07	0.05	5311
ELECTRON MICROSCOPY	r_ncsr_local_group_41	0.047	0.05	5358
ELECTRON MICROSCOPY	r_ncsr_local_group_42	0.084	0.05	5287
ELECTRON MICROSCOPY	r_ncsr_local_group_43	0.073	0.05	5399
ELECTRON MICROSCOPY	r_ncsr_local_group_44	0.069	0.05	5392
ELECTRON MICROSCOPY	r_ncsr_local_group_45	0.1	0.05	5305
ELECTRON MICROSCOPY	r_ncsr_local_group_46	0.087	0.05	5351
ELECTRON MICROSCOPY	r_ncsr_local_group_47	0.082	0.05	5339
ELECTRON MICROSCOPY	r_ncsr_local_group_48	0.011	0.05	5534
ELECTRON MICROSCOPY	r_ncsr_local_group_49	0.073	0.05	5404
ELECTRON MICROSCOPY	r_ncsr_local_group_50	0.07	0.05	5391
ELECTRON MICROSCOPY	r_ncsr_local_group_51	0.094	0.05	5308
ELECTRON MICROSCOPY	r_ncsr_local_group_52	0.077	0.05	5319
ELECTRON MICROSCOPY	r_ncsr_local_group_53	0.047	0.05	5365
ELECTRON MICROSCOPY	r_ncsr_local_group_54	0.084	0.05	5306
ELECTRON MICROSCOPY	r_ncsr_local_group_55	0.059	0.05	5346
ELECTRON MICROSCOPY	r_ncsr_local_group_56	0.055	0.05	5349
ELECTRON MICROSCOPY	r_ncsr_local_group_57	0.006	0.05	5436
ELECTRON MICROSCOPY	r_ncsr_local_group_58	0.077	0.05	5320
ELECTRON MICROSCOPY	r_ncsr_local_group_59	0.048	0.05	5357
ELECTRON MICROSCOPY	r_ncsr_local_group_60	0.084	0.05	5303
ELECTRON MICROSCOPY	r_ncsr_local_group_61	0.076	0.05	5426
ELECTRON MICROSCOPY	r_ncsr_local_group_62	0.102	0.05	5354
ELECTRON MICROSCOPY	r_ncsr_local_group_63	0.079	0.05	5406
ELECTRON MICROSCOPY	r_ncsr_local_group_64	0.083	0.05	5429
ELECTRON MICROSCOPY	r_ncsr_local_group_65	0.073	0.05	5424
ELECTRON MICROSCOPY	r_ncsr_local_group_66	0.006	0.05	5602
ELECTRON MICROSCOPY	r_ncsr_local_group_67	0.077	0.05	5433
ELECTRON MICROSCOPY	r_ncsr_local_group_68	0.097	0.05	5361
ELECTRON MICROSCOPY	r_ncsr_local_group_69	0.073	0.05	5322
ELECTRON MICROSCOPY	r_ncsr_local_group_70	0.089	0.05	5301
ELECTRON MICROSCOPY	r_ncsr_local_group_71	0.075	0.05	5329
ELECTRON MICROSCOPY	r_ncsr_local_group_72	0.072	0.05	5311
ELECTRON MICROSCOPY	r_ncsr_local_group_73	0.077	0.05	5316
ELECTRON MICROSCOPY	r_ncsr_local_group_74	0.007	0.05	5455
ELECTRON MICROSCOPY	r_ncsr_local_group_75	0.074	0.05	5314
ELECTRON MICROSCOPY	r_ncsr_local_group_76	0.089	0.05	5297
ELECTRON MICROSCOPY	r_ncsr_local_group_77	0.101	0.05	5356
ELECTRON MICROSCOPY	r_ncsr_local_group_78	0.087	0.05	5348
ELECTRON MICROSCOPY	r_ncsr_local_group_79	0.088	0.05	5384
ELECTRON MICROSCOPY	r_ncsr_local_group_80	0.068	0.05	5415
ELECTRON MICROSCOPY	r_ncsr_local_group_81	0.077	0.05	5426
ELECTRON MICROSCOPY	r_ncsr_local_group_82	0.006	0.05	5593
ELECTRON MICROSCOPY	r_ncsr_local_group_83	0.093	0.05	5354
ELECTRON MICROSCOPY	r_ncsr_local_group_84	0.083	0.05	5300
ELECTRON MICROSCOPY	r_ncsr_local_group_85	0.053	0.05	5338
ELECTRON MICROSCOPY	r_ncsr_local_group_86	0.047	0.05	5361
ELECTRON MICROSCOPY	r_ncsr_local_group_87	0.047	0.05	5365
ELECTRON MICROSCOPY	r_ncsr_local_group_88	0.073	0.05	5322
ELECTRON MICROSCOPY	r_ncsr_local_group_89	0.006	0.05	5429
ELECTRON MICROSCOPY	r_ncsr_local_group_90	0.083	0.05	5298
ELECTRON MICROSCOPY	r_ncsr_local_group_91	0.105	0.05	5307
ELECTRON MICROSCOPY	r_ncsr_local_group_92	0.094	0.05	5381
ELECTRON MICROSCOPY	r_ncsr_local_group_93	0.101	0.05	5329
ELECTRON MICROSCOPY	r_ncsr_local_group_94	0.102	0.05	5355
ELECTRON MICROSCOPY	r_ncsr_local_group_95	0.101	0.05	5358
ELECTRON MICROSCOPY	r_ncsr_local_group_96	0.058	0.05	5493
ELECTRON MICROSCOPY	r_ncsr_local_group_97	0.087	0.05	5306
ELECTRON MICROSCOPY	r_ncsr_local_group_98	0.084	0.05	5292
ELECTRON MICROSCOPY	r_ncsr_local_group_99	0.083	0.05	5309
ELECTRON MICROSCOPY	r_ncsr_local_group_100	0.088	0.05	5307
ELECTRON MICROSCOPY	r_ncsr_local_group_101	0.083	0.05	5298
ELECTRON MICROSCOPY	r_ncsr_local_group_102	0.007	0.05	5463
ELECTRON MICROSCOPY	r_ncsr_local_group_103	0.093	0.05	5361
ELECTRON MICROSCOPY	r_ncsr_local_group_104	0.086	0.05	5380
ELECTRON MICROSCOPY	r_ncsr_local_group_105	0.079	0.05	5408
ELECTRON MICROSCOPY	r_ncsr_local_group_106	0.088	0.05	5361
ELECTRON MICROSCOPY	r_ncsr_local_group_107	0.104	0.05	5283
ELECTRON MICROSCOPY	r_ncsr_local_group_108	0.058	0.05	5320
ELECTRON MICROSCOPY	r_ncsr_local_group_109	0.059	0.05	5353
ELECTRON MICROSCOPY	r_ncsr_local_group_110	0.074	0.05	5339
ELECTRON MICROSCOPY	r_ncsr_local_group_111	0.053	0.05	5337
ELECTRON MICROSCOPY	r_ncsr_local_group_112	0.087	0.05	5295
ELECTRON MICROSCOPY	r_ncsr_local_group_113	0.082	0.05	5369
ELECTRON MICROSCOPY	r_ncsr_local_group_114	0.083	0.05	5433
ELECTRON MICROSCOPY	r_ncsr_local_group_115	0.089	0.05	5396
ELECTRON MICROSCOPY	r_ncsr_local_group_116	0.086	0.05	5402
ELECTRON MICROSCOPY	r_ncsr_local_group_117	0.056	0.05	5354
ELECTRON MICROSCOPY	r_ncsr_local_group_118	0.071	0.05	5316
ELECTRON MICROSCOPY	r_ncsr_local_group_119	0.047	0.05	5360
ELECTRON MICROSCOPY	r_ncsr_local_group_120	0.083	0.05	5285
ELECTRON MICROSCOPY	r_ncsr_local_group_121	0.073	0.05	5433
ELECTRON MICROSCOPY	r_ncsr_local_group_122	0.068	0.05	5421
ELECTRON MICROSCOPY	r_ncsr_local_group_123	0.093	0.05	5320
ELECTRON MICROSCOPY	r_ncsr_local_group_124	0.077	0.05	5325
ELECTRON MICROSCOPY	r_ncsr_local_group_125	0.047	0.05	5363
ELECTRON MICROSCOPY	r_ncsr_local_group_126	0.083	0.05	5304
ELECTRON MICROSCOPY	r_ncsr_local_group_127	0.077	0.05	5434
ELECTRON MICROSCOPY	r_ncsr_local_group_128	0.095	0.05	5359
ELECTRON MICROSCOPY	r_ncsr_local_group_129	0.073	0.05	5322
ELECTRON MICROSCOPY	r_ncsr_local_group_130	0.088	0.05	5298
ELECTRON MICROSCOPY	r_ncsr_local_group_131	0.094	0.05	5360
ELECTRON MICROSCOPY	r_ncsr_local_group_132	0.083	0.05	5289

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Refine-ID	Type	Rms dev position (Å)	Weight position
1	1	A	ELECTRON MICROSCOPY	Local ncs	0.09589	0.05009
1	2	A	ELECTRON MICROSCOPY	Local ncs	0.09589	0.05009
2	3	A	ELECTRON MICROSCOPY	Local ncs	0.08197	0.05009
2	4	A	ELECTRON MICROSCOPY	Local ncs	0.08197	0.05009
3	5	A	ELECTRON MICROSCOPY	Local ncs	0.07343	0.05009
3	6	A	ELECTRON MICROSCOPY	Local ncs	0.07343	0.05009
4	7	A	ELECTRON MICROSCOPY	Local ncs	0.08186	0.05009
4	8	A	ELECTRON MICROSCOPY	Local ncs	0.08186	0.05009
5	9	A	ELECTRON MICROSCOPY	Local ncs	0.10393	0.05008
5	10	A	ELECTRON MICROSCOPY	Local ncs	0.10393	0.05008
6	11	A	ELECTRON MICROSCOPY	Local ncs	0.0453	0.0501
6	12	A	ELECTRON MICROSCOPY	Local ncs	0.0453	0.0501
7	13	A	ELECTRON MICROSCOPY	Local ncs	0.08618	0.05009
7	14	A	ELECTRON MICROSCOPY	Local ncs	0.08618	0.05009
8	15	A	ELECTRON MICROSCOPY	Local ncs	0.08042	0.05009
8	16	A	ELECTRON MICROSCOPY	Local ncs	0.08042	0.05009
9	17	A	ELECTRON MICROSCOPY	Local ncs	0.07316	0.05009
9	18	A	ELECTRON MICROSCOPY	Local ncs	0.07316	0.05009
10	19	A	ELECTRON MICROSCOPY	Local ncs	0.08302	0.05009
10	20	A	ELECTRON MICROSCOPY	Local ncs	0.08302	0.05009
11	21	A	ELECTRON MICROSCOPY	Local ncs	0.09898	0.05008
11	22	A	ELECTRON MICROSCOPY	Local ncs	0.09898	0.05008
12	23	A	ELECTRON MICROSCOPY	Local ncs	0.05727	0.0501
12	24	A	ELECTRON MICROSCOPY	Local ncs	0.05727	0.0501
13	25	A	ELECTRON MICROSCOPY	Local ncs	0.05812	0.0501
13	26	A	ELECTRON MICROSCOPY	Local ncs	0.05812	0.0501
14	27	A	ELECTRON MICROSCOPY	Local ncs	0.0746	0.0501
14	28	A	ELECTRON MICROSCOPY	Local ncs	0.0746	0.0501
15	29	A	ELECTRON MICROSCOPY	Local ncs	0.0516	0.0501
15	30	A	ELECTRON MICROSCOPY	Local ncs	0.0516	0.0501
16	31	A	ELECTRON MICROSCOPY	Local ncs	0.0877	0.0501
16	32	A	ELECTRON MICROSCOPY	Local ncs	0.0877	0.0501
17	33	A	ELECTRON MICROSCOPY	Local ncs	0.00582	0.05011
17	34	A	ELECTRON MICROSCOPY	Local ncs	0.00582	0.05011
18	35	A	ELECTRON MICROSCOPY	Local ncs	0.0581	0.0501
18	36	A	ELECTRON MICROSCOPY	Local ncs	0.0581	0.0501
19	37	A	ELECTRON MICROSCOPY	Local ncs	0.05795	0.0501
19	38	A	ELECTRON MICROSCOPY	Local ncs	0.05795	0.0501
20	39	A	ELECTRON MICROSCOPY	Local ncs	0.07449	0.0501
20	40	A	ELECTRON MICROSCOPY	Local ncs	0.07449	0.0501
21	41	A	ELECTRON MICROSCOPY	Local ncs	0.0519	0.0501
21	42	A	ELECTRON MICROSCOPY	Local ncs	0.0519	0.0501
22	43	A	ELECTRON MICROSCOPY	Local ncs	0.08776	0.0501
22	44	A	ELECTRON MICROSCOPY	Local ncs	0.08776	0.0501
23	45	A	ELECTRON MICROSCOPY	Local ncs	0.09226	0.05008
23	46	A	ELECTRON MICROSCOPY	Local ncs	0.09226	0.05008
24	47	A	ELECTRON MICROSCOPY	Local ncs	0.0902	0.05009
24	48	A	ELECTRON MICROSCOPY	Local ncs	0.0902	0.05009
25	49	A	ELECTRON MICROSCOPY	Local ncs	0.09475	0.05009
25	50	A	ELECTRON MICROSCOPY	Local ncs	0.09475	0.05009
26	51	A	ELECTRON MICROSCOPY	Local ncs	0.10337	0.05009
26	52	A	ELECTRON MICROSCOPY	Local ncs	0.10337	0.05009
27	53	A	ELECTRON MICROSCOPY	Local ncs	0.10224	0.05008
27	54	A	ELECTRON MICROSCOPY	Local ncs	0.10224	0.05008
28	55	A	ELECTRON MICROSCOPY	Local ncs	0.05308	0.0501
28	56	A	ELECTRON MICROSCOPY	Local ncs	0.05308	0.0501
29	57	A	ELECTRON MICROSCOPY	Local ncs	0.09154	0.05009
29	58	A	ELECTRON MICROSCOPY	Local ncs	0.09154	0.05009
30	59	A	ELECTRON MICROSCOPY	Local ncs	0.08991	0.05009
30	60	A	ELECTRON MICROSCOPY	Local ncs	0.08991	0.05009
31	61	A	ELECTRON MICROSCOPY	Local ncs	0.09551	0.05009
31	62	A	ELECTRON MICROSCOPY	Local ncs	0.09551	0.05009
32	63	A	ELECTRON MICROSCOPY	Local ncs	0.09662	0.05009
32	64	A	ELECTRON MICROSCOPY	Local ncs	0.09662	0.05009
33	65	A	ELECTRON MICROSCOPY	Local ncs	0.05421	0.05011
33	66	A	ELECTRON MICROSCOPY	Local ncs	0.05421	0.05011
34	67	A	ELECTRON MICROSCOPY	Local ncs	0.0705	0.0501
34	68	A	ELECTRON MICROSCOPY	Local ncs	0.0705	0.0501
35	69	A	ELECTRON MICROSCOPY	Local ncs	0.04634	0.0501
35	70	A	ELECTRON MICROSCOPY	Local ncs	0.04634	0.0501
36	71	A	ELECTRON MICROSCOPY	Local ncs	0.08314	0.0501
36	72	A	ELECTRON MICROSCOPY	Local ncs	0.08314	0.0501
37	73	A	ELECTRON MICROSCOPY	Local ncs	0.05773	0.0501
37	74	A	ELECTRON MICROSCOPY	Local ncs	0.05773	0.0501
38	75	A	ELECTRON MICROSCOPY	Local ncs	0.0051	0.05011
38	76	A	ELECTRON MICROSCOPY	Local ncs	0.0051	0.05011
39	77	A	ELECTRON MICROSCOPY	Local ncs	0.05466	0.0501
39	78	A	ELECTRON MICROSCOPY	Local ncs	0.05466	0.0501
40	79	A	ELECTRON MICROSCOPY	Local ncs	0.06995	0.0501
40	80	A	ELECTRON MICROSCOPY	Local ncs	0.06995	0.0501
41	81	A	ELECTRON MICROSCOPY	Local ncs	0.04663	0.0501
41	82	A	ELECTRON MICROSCOPY	Local ncs	0.04663	0.0501
42	83	A	ELECTRON MICROSCOPY	Local ncs	0.08408	0.0501
42	84	A	ELECTRON MICROSCOPY	Local ncs	0.08408	0.0501
43	85	A	ELECTRON MICROSCOPY	Local ncs	0.07275	0.05009
43	86	A	ELECTRON MICROSCOPY	Local ncs	0.07275	0.05009
44	87	A	ELECTRON MICROSCOPY	Local ncs	0.0695	0.05009
44	88	A	ELECTRON MICROSCOPY	Local ncs	0.0695	0.05009
45	89	A	ELECTRON MICROSCOPY	Local ncs	0.10022	0.05008
45	90	A	ELECTRON MICROSCOPY	Local ncs	0.10022	0.05008
46	91	A	ELECTRON MICROSCOPY	Local ncs	0.08687	0.05009
46	92	A	ELECTRON MICROSCOPY	Local ncs	0.08687	0.05009
47	93	A	ELECTRON MICROSCOPY	Local ncs	0.08223	0.05009
47	94	A	ELECTRON MICROSCOPY	Local ncs	0.08223	0.05009
48	95	A	ELECTRON MICROSCOPY	Local ncs	0.01142	0.0501
48	96	A	ELECTRON MICROSCOPY	Local ncs	0.01142	0.0501
49	97	A	ELECTRON MICROSCOPY	Local ncs	0.07251	0.05009
49	98	A	ELECTRON MICROSCOPY	Local ncs	0.07251	0.05009
50	99	A	ELECTRON MICROSCOPY	Local ncs	0.06952	0.05009
50	100	A	ELECTRON MICROSCOPY	Local ncs	0.06952	0.05009
51	101	A	ELECTRON MICROSCOPY	Local ncs	0.09361	0.05008
51	102	A	ELECTRON MICROSCOPY	Local ncs	0.09361	0.05008
52	103	A	ELECTRON MICROSCOPY	Local ncs	0.07721	0.0501
52	104	A	ELECTRON MICROSCOPY	Local ncs	0.07721	0.0501
53	105	A	ELECTRON MICROSCOPY	Local ncs	0.04715	0.0501
53	106	A	ELECTRON MICROSCOPY	Local ncs	0.04715	0.0501
54	107	A	ELECTRON MICROSCOPY	Local ncs	0.08423	0.0501
54	108	A	ELECTRON MICROSCOPY	Local ncs	0.08423	0.0501
55	109	A	ELECTRON MICROSCOPY	Local ncs	0.05943	0.0501
55	110	A	ELECTRON MICROSCOPY	Local ncs	0.05943	0.0501
56	111	A	ELECTRON MICROSCOPY	Local ncs	0.05547	0.0501
56	112	A	ELECTRON MICROSCOPY	Local ncs	0.05547	0.0501
57	113	A	ELECTRON MICROSCOPY	Local ncs	0.00576	0.05011
57	114	A	ELECTRON MICROSCOPY	Local ncs	0.00576	0.05011
58	115	A	ELECTRON MICROSCOPY	Local ncs	0.07705	0.0501
58	116	A	ELECTRON MICROSCOPY	Local ncs	0.07705	0.0501
59	117	A	ELECTRON MICROSCOPY	Local ncs	0.04765	0.0501
59	118	A	ELECTRON MICROSCOPY	Local ncs	0.04765	0.0501
60	119	A	ELECTRON MICROSCOPY	Local ncs	0.08421	0.0501
60	120	A	ELECTRON MICROSCOPY	Local ncs	0.08421	0.0501
61	121	A	ELECTRON MICROSCOPY	Local ncs	0.07561	0.05009
61	122	A	ELECTRON MICROSCOPY	Local ncs	0.07561	0.05009
62	123	A	ELECTRON MICROSCOPY	Local ncs	0.10224	0.05009
62	124	A	ELECTRON MICROSCOPY	Local ncs	0.10224	0.05009
63	125	A	ELECTRON MICROSCOPY	Local ncs	0.0794	0.05009
63	126	A	ELECTRON MICROSCOPY	Local ncs	0.0794	0.05009
64	127	A	ELECTRON MICROSCOPY	Local ncs	0.08348	0.05009
64	128	A	ELECTRON MICROSCOPY	Local ncs	0.08348	0.05009
65	129	A	ELECTRON MICROSCOPY	Local ncs	0.0729	0.05009
65	130	A	ELECTRON MICROSCOPY	Local ncs	0.0729	0.05009
66	131	A	ELECTRON MICROSCOPY	Local ncs	0.0057	0.0501
66	132	A	ELECTRON MICROSCOPY	Local ncs	0.0057	0.0501
67	133	A	ELECTRON MICROSCOPY	Local ncs	0.07718	0.05009
67	134	A	ELECTRON MICROSCOPY	Local ncs	0.07718	0.05009
68	135	A	ELECTRON MICROSCOPY	Local ncs	0.09723	0.05009
68	136	A	ELECTRON MICROSCOPY	Local ncs	0.09723	0.05009
69	137	A	ELECTRON MICROSCOPY	Local ncs	0.07334	0.0501
69	138	A	ELECTRON MICROSCOPY	Local ncs	0.07334	0.0501
70	139	A	ELECTRON MICROSCOPY	Local ncs	0.08928	0.0501
70	140	A	ELECTRON MICROSCOPY	Local ncs	0.08928	0.0501
71	141	A	ELECTRON MICROSCOPY	Local ncs	0.07468	0.0501
71	142	A	ELECTRON MICROSCOPY	Local ncs	0.07468	0.0501
72	143	A	ELECTRON MICROSCOPY	Local ncs	0.07162	0.0501
72	144	A	ELECTRON MICROSCOPY	Local ncs	0.07162	0.0501
73	145	A	ELECTRON MICROSCOPY	Local ncs	0.07704	0.0501
73	146	A	ELECTRON MICROSCOPY	Local ncs	0.07704	0.0501
74	147	A	ELECTRON MICROSCOPY	Local ncs	0.0066	0.05011
74	148	A	ELECTRON MICROSCOPY	Local ncs	0.0066	0.05011
75	149	A	ELECTRON MICROSCOPY	Local ncs	0.07356	0.0501
75	150	A	ELECTRON MICROSCOPY	Local ncs	0.07356	0.0501
76	151	A	ELECTRON MICROSCOPY	Local ncs	0.08902	0.0501
76	152	A	ELECTRON MICROSCOPY	Local ncs	0.08902	0.0501
77	153	A	ELECTRON MICROSCOPY	Local ncs	0.10081	0.05009
77	154	A	ELECTRON MICROSCOPY	Local ncs	0.10081	0.05009
78	155	A	ELECTRON MICROSCOPY	Local ncs	0.08707	0.05009
78	156	A	ELECTRON MICROSCOPY	Local ncs	0.08707	0.05009
79	157	A	ELECTRON MICROSCOPY	Local ncs	0.0879	0.05009
79	158	A	ELECTRON MICROSCOPY	Local ncs	0.0879	0.05009
80	159	A	ELECTRON MICROSCOPY	Local ncs	0.06822	0.05009
80	160	A	ELECTRON MICROSCOPY	Local ncs	0.06822	0.05009
81	161	A	ELECTRON MICROSCOPY	Local ncs	0.07671	0.05009
81	162	A	ELECTRON MICROSCOPY	Local ncs	0.07671	0.05009
82	163	A	ELECTRON MICROSCOPY	Local ncs	0.00628	0.0501
82	164	A	ELECTRON MICROSCOPY	Local ncs	0.00628	0.0501
83	165	A	ELECTRON MICROSCOPY	Local ncs	0.09297	0.05009
83	166	A	ELECTRON MICROSCOPY	Local ncs	0.09297	0.05009
84	167	A	ELECTRON MICROSCOPY	Local ncs	0.08329	0.0501
84	168	A	ELECTRON MICROSCOPY	Local ncs	0.08329	0.0501
85	169	A	ELECTRON MICROSCOPY	Local ncs	0.05295	0.0501
85	170	A	ELECTRON MICROSCOPY	Local ncs	0.05295	0.0501
86	171	A	ELECTRON MICROSCOPY	Local ncs	0.04679	0.0501
86	172	A	ELECTRON MICROSCOPY	Local ncs	0.04679	0.0501
87	173	A	ELECTRON MICROSCOPY	Local ncs	0.04705	0.0501
87	174	A	ELECTRON MICROSCOPY	Local ncs	0.04705	0.0501
88	175	A	ELECTRON MICROSCOPY	Local ncs	0.07282	0.0501
88	176	A	ELECTRON MICROSCOPY	Local ncs	0.07282	0.0501
89	177	A	ELECTRON MICROSCOPY	Local ncs	0.00631	0.05011
89	178	A	ELECTRON MICROSCOPY	Local ncs	0.00631	0.05011
90	179	A	ELECTRON MICROSCOPY	Local ncs	0.08286	0.0501
90	180	A	ELECTRON MICROSCOPY	Local ncs	0.08286	0.0501
91	181	A	ELECTRON MICROSCOPY	Local ncs	0.10482	0.05008
91	182	A	ELECTRON MICROSCOPY	Local ncs	0.10482	0.05008
92	183	A	ELECTRON MICROSCOPY	Local ncs	0.09439	0.05009
92	184	A	ELECTRON MICROSCOPY	Local ncs	0.09439	0.05009
93	185	A	ELECTRON MICROSCOPY	Local ncs	0.10095	0.05009
93	186	A	ELECTRON MICROSCOPY	Local ncs	0.10095	0.05009
94	187	A	ELECTRON MICROSCOPY	Local ncs	0.1018	0.05009
94	188	A	ELECTRON MICROSCOPY	Local ncs	0.1018	0.05009
95	189	A	ELECTRON MICROSCOPY	Local ncs	0.10136	0.05009
95	190	A	ELECTRON MICROSCOPY	Local ncs	0.10136	0.05009
96	191	A	ELECTRON MICROSCOPY	Local ncs	0.0578	0.05009
96	192	A	ELECTRON MICROSCOPY	Local ncs	0.0578	0.05009
97	193	A	ELECTRON MICROSCOPY	Local ncs	0.08728	0.0501
97	194	A	ELECTRON MICROSCOPY	Local ncs	0.08728	0.0501
98	195	A	ELECTRON MICROSCOPY	Local ncs	0.08352	0.0501
98	196	A	ELECTRON MICROSCOPY	Local ncs	0.08352	0.0501
99	197	A	ELECTRON MICROSCOPY	Local ncs	0.08303	0.0501
99	198	A	ELECTRON MICROSCOPY	Local ncs	0.08303	0.0501
100	199	A	ELECTRON MICROSCOPY	Local ncs	0.08835	0.0501
100	200	A	ELECTRON MICROSCOPY	Local ncs	0.08835	0.0501
101	201	A	ELECTRON MICROSCOPY	Local ncs	0.08338	0.0501
101	202	A	ELECTRON MICROSCOPY	Local ncs	0.08338	0.0501
102	203	A	ELECTRON MICROSCOPY	Local ncs	0.00706	0.05011
102	204	A	ELECTRON MICROSCOPY	Local ncs	0.00706	0.05011
103	205	A	ELECTRON MICROSCOPY	Local ncs	0.09331	0.05009
103	206	A	ELECTRON MICROSCOPY	Local ncs	0.09331	0.05009
104	207	A	ELECTRON MICROSCOPY	Local ncs	0.08636	0.05009
104	208	A	ELECTRON MICROSCOPY	Local ncs	0.08636	0.05009
105	209	A	ELECTRON MICROSCOPY	Local ncs	0.07912	0.05009
105	210	A	ELECTRON MICROSCOPY	Local ncs	0.07912	0.05009
106	211	A	ELECTRON MICROSCOPY	Local ncs	0.08796	0.05009
106	212	A	ELECTRON MICROSCOPY	Local ncs	0.08796	0.05009
107	213	A	ELECTRON MICROSCOPY	Local ncs	0.1043	0.05008
107	214	A	ELECTRON MICROSCOPY	Local ncs	0.1043	0.05008
108	215	A	ELECTRON MICROSCOPY	Local ncs	0.05839	0.0501
108	216	A	ELECTRON MICROSCOPY	Local ncs	0.05839	0.0501
109	217	A	ELECTRON MICROSCOPY	Local ncs	0.05905	0.0501
109	218	A	ELECTRON MICROSCOPY	Local ncs	0.05905	0.0501
110	219	A	ELECTRON MICROSCOPY	Local ncs	0.07439	0.0501
110	220	A	ELECTRON MICROSCOPY	Local ncs	0.07439	0.0501
111	221	A	ELECTRON MICROSCOPY	Local ncs	0.05303	0.0501
111	222	A	ELECTRON MICROSCOPY	Local ncs	0.05303	0.0501
112	223	A	ELECTRON MICROSCOPY	Local ncs	0.08749	0.0501
112	224	A	ELECTRON MICROSCOPY	Local ncs	0.08749	0.0501
113	225	A	ELECTRON MICROSCOPY	Local ncs	0.08151	0.05009
113	226	A	ELECTRON MICROSCOPY	Local ncs	0.08151	0.05009
114	227	A	ELECTRON MICROSCOPY	Local ncs	0.08294	0.05009
114	228	A	ELECTRON MICROSCOPY	Local ncs	0.08294	0.05009
115	229	A	ELECTRON MICROSCOPY	Local ncs	0.08871	0.05009
115	230	A	ELECTRON MICROSCOPY	Local ncs	0.08871	0.05009
116	231	A	ELECTRON MICROSCOPY	Local ncs	0.08596	0.05009
116	232	A	ELECTRON MICROSCOPY	Local ncs	0.08596	0.05009
117	233	A	ELECTRON MICROSCOPY	Local ncs	0.05573	0.0501
117	234	A	ELECTRON MICROSCOPY	Local ncs	0.05573	0.0501
118	235	A	ELECTRON MICROSCOPY	Local ncs	0.07089	0.0501
118	236	A	ELECTRON MICROSCOPY	Local ncs	0.07089	0.0501
119	237	A	ELECTRON MICROSCOPY	Local ncs	0.0468	0.0501
119	238	A	ELECTRON MICROSCOPY	Local ncs	0.0468	0.0501
120	239	A	ELECTRON MICROSCOPY	Local ncs	0.08347	0.0501
120	240	A	ELECTRON MICROSCOPY	Local ncs	0.08347	0.0501
121	241	A	ELECTRON MICROSCOPY	Local ncs	0.07259	0.05009
121	242	A	ELECTRON MICROSCOPY	Local ncs	0.07259	0.05009
122	243	A	ELECTRON MICROSCOPY	Local ncs	0.06807	0.0501
122	244	A	ELECTRON MICROSCOPY	Local ncs	0.06807	0.0501
123	245	A	ELECTRON MICROSCOPY	Local ncs	0.09333	0.05009
123	246	A	ELECTRON MICROSCOPY	Local ncs	0.09333	0.05009
124	247	A	ELECTRON MICROSCOPY	Local ncs	0.07679	0.0501
124	248	A	ELECTRON MICROSCOPY	Local ncs	0.07679	0.0501
125	249	A	ELECTRON MICROSCOPY	Local ncs	0.04733	0.0501
125	250	A	ELECTRON MICROSCOPY	Local ncs	0.04733	0.0501
126	251	A	ELECTRON MICROSCOPY	Local ncs	0.08315	0.0501
126	252	A	ELECTRON MICROSCOPY	Local ncs	0.08315	0.0501
127	253	A	ELECTRON MICROSCOPY	Local ncs	0.07706	0.05009
127	254	A	ELECTRON MICROSCOPY	Local ncs	0.07706	0.05009
128	255	A	ELECTRON MICROSCOPY	Local ncs	0.0954	0.05009
128	256	A	ELECTRON MICROSCOPY	Local ncs	0.0954	0.05009
129	257	A	ELECTRON MICROSCOPY	Local ncs	0.07283	0.0501
129	258	A	ELECTRON MICROSCOPY	Local ncs	0.07283	0.0501
130	259	A	ELECTRON MICROSCOPY	Local ncs	0.08822	0.0501
130	260	A	ELECTRON MICROSCOPY	Local ncs	0.08822	0.0501
131	261	A	ELECTRON MICROSCOPY	Local ncs	0.09359	0.05009
131	262	A	ELECTRON MICROSCOPY	Local ncs	0.09359	0.05009
132	263	A	ELECTRON MICROSCOPY	Local ncs	0.0832	0.0501
132	264	A	ELECTRON MICROSCOPY	Local ncs	0.0832	0.0501

LS refinement shell

Refine-ID: ELECTRON MICROSCOPY / Num. reflection R_free: _ / Total num. of bins used: 20 / % reflection obs: 100 %

Resolution (Å)	Rfactor Rwork	Num. reflection Rwork	Rfactor all	Num. reflection all	Fsc work	WRfactor Rwork
3.15-3.232	0.62	16091	0.62	16091	0.571	0.62
3.232-3.32	0.436	15581	0.436	15581	0.677	0.436
3.32-3.417	0.399	15274	0.399	15274	0.73	0.399
3.417-3.522	0.388	14841	0.388	14841	0.756	0.388
3.522-3.637	0.366	14269	0.366	14269	0.789	0.366
3.637-3.765	0.339	13908	0.339	13908	0.827	0.339
3.765-3.907	0.324	13387	0.324	13387	0.847	0.324
3.907-4.066	0.315	12916	0.315	12916	0.856	0.315
4.066-4.247	0.299	12257	0.299	12257	0.878	0.299
4.247-4.454	0.289	11832	0.289	11832	0.889	0.289
4.454-4.695	0.282	11220	0.282	11220	0.894	0.282
4.695-4.979	0.278	10583	0.278	10583	0.894	0.278
4.979-5.323	0.278	9964	0.278	9964	0.891	0.278
5.323-5.748	0.299	9314	0.299	9314	0.885	0.299
5.748-6.296	0.331	8555	0.331	8555	0.873	0.331
6.296-7.038	0.306	7708	0.306	7708	0.884	0.306
7.038-8.124	0.269	6837	0.269	6837	0.897	0.269
8.124-9.943	0.24	5727	0.24	5727	0.93	0.24
9.943-14.034	0.224	4463	0.224	4463	0.947	0.224
14.034-157.32	0.231	2455	0.231	2455	0.983	0.231