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- PDB-9oop: Human delta 2 receptor with R710W Cerebellar Ataxia mutation in t... -

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Basic information

Entry
Database: PDB / ID: 9oop
TitleHuman delta 2 receptor with R710W Cerebellar Ataxia mutation in the apo leak state
ComponentsHuman delta 2 receptor with R710W Cerebellar Ataxia mutation
KeywordsTRANSPORT PROTEIN / Ligand-gated ion channel / ion channel / neurotransmitter receptor
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.73 Å
AuthorsWang, H. / Ahmed, F. / Khau, J. / Mondal, A.K. / Twomey, E.C.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM154904 United States
CitationJournal: To Be Published
Title: Human Delta-2 receptors are ligand-gated ion channels
Authors: Wang, H. / Ahmed, F. / Khau, J. / Mondal, A.K. / Twomey, E.C.
History
DepositionMay 16, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 24, 2025Provider: repository / Type: Initial release
Revision 1.0Sep 24, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Sep 24, 2025Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Human delta 2 receptor with R710W Cerebellar Ataxia mutation
B: Human delta 2 receptor with R710W Cerebellar Ataxia mutation
C: Human delta 2 receptor with R710W Cerebellar Ataxia mutation
D: Human delta 2 receptor with R710W Cerebellar Ataxia mutation


Theoretical massNumber of molelcules
Total (without water)321,4524
Polymers321,4524
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: Protein
Human delta 2 receptor with R710W Cerebellar Ataxia mutation


Mass: 80363.031 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Human Delta-2 Receptor with the R710W cerebellar ataxia mutation
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 900 nm
Image recordingElectron dose: 45 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

EM softwareName: PHENIX / Version: 1.21.1_5286 / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.73 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 131121 / Symmetry type: POINT
RefinementHighest resolution: 3.73 Å
Stereochemistry target values: REAL-SPACE (WEIGHTED MAP SUM AT ATOM CENTERS)
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00322912
ELECTRON MICROSCOPYf_angle_d0.71131050
ELECTRON MICROSCOPYf_dihedral_angle_d7.7333076
ELECTRON MICROSCOPYf_chiral_restr0.0483458
ELECTRON MICROSCOPYf_plane_restr0.0094022

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