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- PDB-9h33: T2R-TTL-PROTAC2 complex -

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Basic information

Entry
Database: PDB / ID: 9h33
TitleT2R-TTL-PROTAC2 complex
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
  • Tubulin--tyrosine ligase
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE
Function / homology
Function and homology information


tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / cellular response to interleukin-4 / tubulin binding ...tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / cellular response to interleukin-4 / tubulin binding / positive regulation of mitotic cell cycle / post-translational protein modification / spindle microtubule / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration / neuron projection development / mitotic cell cycle / double-stranded RNA binding / microtubule cytoskeleton / growth cone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / neuron projection / cilium / protein heterodimerization activity / GTPase activity / ubiquitin protein ligase binding / GTP binding / Golgi apparatus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
: / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. ...: / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
: / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Tubulin--tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain / Tubulin beta-2B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Bos taurus (domestic cattle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.3 Å
AuthorsMaiocchi, A. / Abel, A.-C. / Boiarska, Z. / Steinmetz, M.O. / Passarella, D. / Prota, A.E.
Funding supportEuropean Union, Switzerland, 2items
OrganizationGrant numberCountry
European Union (EU)860070 TubInTrainEuropean Union
Swiss National Science Foundation31003A_166608 Switzerland
Citation
Journal: To Be Published
Title: Effective tubulin degradation by rationally designed Proteolysis Targeting Chimeras
Authors: Maiocchi, A. / Abel, A.-C. / Basellini, M. / Perez Pena, H. / Boiarska, Z. / Ferrandi, E. / Kozicka, Z. / Fasano, V. / Pieraccini, S. / Cappelletti, G. / Steinmetz, M.O. / Prota, A.E. / Passarella, D.
#1: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionOct 15, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 29, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta-2B chain
C: Tubulin alpha-1B chain
D: Tubulin beta-2B chain
E: Stathmin-4
F: Tubulin--tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)265,65323
Polymers261,5746
Non-polymers4,07917
Water4,918273
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area23490 Å2
ΔGint-149 kcal/mol
Surface area81260 Å2
MethodPISA
Unit cell
Length a, b, c (Å)104.960, 156.660, 180.950
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 50204.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: brain / References: UniProt: P81947
#2: Protein Tubulin beta-2B chain


Mass: 49999.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: brain / References: UniProt: Q6B856
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16787.111 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli (E. coli) / References: UniProt: P63043
#4: Protein Tubulin--tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli (E. coli) / References: UniProt: A0A8V0Z8P0, tubulin-tyrosine ligase

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Non-polymers , 9 types, 290 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Mg
#7: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#9: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#10: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#11: Chemical ChemComp-A1ISC / (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl 5-{1-[6-({(4R)-2-[(3R)-2,6-dioxopiperidin-3-yl]-1-oxo-2,4-dihydro-1H-isoindol-4-yl}amino)-6-oxohexyl]-1H-1,2,3-triazol-4-yl}pentanoate


Mass: 1071.609 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C54H67ClN8O13 / Feature type: SUBJECT OF INVESTIGATION
#12: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 273 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.84 Å3/Da / Density % sol: 56.74 %
Crystal growTemperature: 293 K / Method: vapor diffusion / pH: 6.5
Details: 4% PEG 4K, 13% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1.00003 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 2, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.00003 Å / Relative weight: 1
ReflectionResolution: 2.3→49.61 Å / Num. obs: 132666 / % possible obs: 99.97 % / Redundancy: 27.5 % / Biso Wilson estimate: 59.25 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.1418 / Rpim(I) all: 0.0274 / Rrim(I) all: 0.1444 / Net I/σ(I): 15.92
Reflection shellResolution: 2.3→2.33 Å / Rmerge(I) obs: 3.556 / Mean I/σ(I) obs: 0.81 / Num. unique obs: 4382 / CC1/2: 0.447 / Rpim(I) all: 0.6818 / Rrim(I) all: 3.621

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Processing

Software
NameVersionClassification
PHENIX1.21.2_5419refinement
XDSdata reduction
XSCALEdata scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 2.3→49.61 Å / SU ML: 0.3315 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.8986
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2278 6634 5 %
Rwork0.1987 126018 -
obs0.2001 132652 99.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 76.39 Å2
Refinement stepCycle: LAST / Resolution: 2.3→49.61 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17378 0 254 273 17905
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.001918045
X-RAY DIFFRACTIONf_angle_d0.671324467
X-RAY DIFFRACTIONf_chiral_restr0.04042661
X-RAY DIFFRACTIONf_plane_restr0.00333165
X-RAY DIFFRACTIONf_dihedral_angle_d12.11566694
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.330.30562190.31124163X-RAY DIFFRACTION99.77
2.33-2.350.32772180.30984127X-RAY DIFFRACTION100
2.35-2.380.35472200.30714183X-RAY DIFFRACTION99.98
2.38-2.410.38712180.33354146X-RAY DIFFRACTION100
2.41-2.440.38552200.33624190X-RAY DIFFRACTION100
2.44-2.480.32642160.29014102X-RAY DIFFRACTION99.91
2.48-2.510.30692210.29284199X-RAY DIFFRACTION100
2.51-2.550.32190.26164160X-RAY DIFFRACTION99.98
2.55-2.590.26042170.23934125X-RAY DIFFRACTION99.98
2.59-2.630.26982200.24124179X-RAY DIFFRACTION99.98
2.63-2.680.28952190.23834157X-RAY DIFFRACTION100
2.68-2.730.27422200.23864175X-RAY DIFFRACTION99.98
2.73-2.780.27282190.24514158X-RAY DIFFRACTION100
2.78-2.840.26642210.24154206X-RAY DIFFRACTION99.98
2.84-2.90.27912210.25264184X-RAY DIFFRACTION100
2.9-2.970.28242180.25774148X-RAY DIFFRACTION100
2.97-3.040.29662210.24314207X-RAY DIFFRACTION100
3.04-3.120.27142210.23454188X-RAY DIFFRACTION100
3.12-3.210.25442190.23154170X-RAY DIFFRACTION100
3.21-3.320.27662220.2234218X-RAY DIFFRACTION100
3.32-3.440.26072200.21584181X-RAY DIFFRACTION99.98
3.44-3.570.25872220.20884205X-RAY DIFFRACTION100
3.57-3.740.22482220.19854226X-RAY DIFFRACTION99.89
3.74-3.930.22220.17534221X-RAY DIFFRACTION100
3.93-4.180.18532220.16954220X-RAY DIFFRACTION100
4.18-4.50.17232230.15714234X-RAY DIFFRACTION100
4.5-4.950.16922240.14784261X-RAY DIFFRACTION100
4.95-5.670.22092260.17624285X-RAY DIFFRACTION100
5.67-7.140.21172260.19124302X-RAY DIFFRACTION99.87
7.14-49.610.18382380.16084498X-RAY DIFFRACTION99.77
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.41646690899-0.568733289109-0.6765048529093.04454469289-0.1706947988921.763345277910.07581927671040.01004999851540.377714831038-0.5027970520880.212230593085-0.238897013092-1.021028564780.280380426357-0.3044135267130.982765676105-0.1659414029770.1736586952020.629520231391-0.153030823360.61070252042329.10452757197.087094965853.936288152
21.6878582245-0.0332752733969-0.3540132051673.022346089681.483163384513.26076491797-0.0425173371930.03533175873330.0260706969981-0.08189063846860.299542133811-0.328869037812-0.3756114695260.495817820552-0.2444546942710.484630254371-0.07374542788790.04580352112020.50850367569-0.1345912044120.52967939886528.815095096181.55267330956.6593254368
31.570223985571.40507782344-0.2122523649542.104715903051.098713051895.183528090350.0633356037714-0.2575704710320.04570346624510.474461566256-0.1396505554120.751561350721-0.380440099236-0.6636010977340.04410096603730.5082442269710.01938116787880.1424027281630.476711692023-0.1381955997690.5958480242379.2524850731184.236392662771.5182000424
40.946884185571-0.4398178288130.05948592053813.955786340762.246575053473.44776721519-0.0743767184829-0.1440557876710.03276604131680.7681554967370.225160721613-0.01748501314960.2548080835460.262808698171-0.1546786123170.492608484917-0.03237039612820.05918265672110.500712733509-0.08531219320480.51375861565723.026365190476.487376405669.8850712106
54.58982582492-1.33393204773-0.1389123528474.09756701360.5111700007674.361074739610.06626939267330.3153620585650.611746023354-0.44269889457-0.09734210583930.213404173874-0.887309778322-0.2257229079410.06219188647940.6215732561570.05598179903720.02424049075090.4941748359040.001589706952380.59610749294716.26252969969.816052730219.2062613592
61.4142804488-0.481016117073-0.3013905155422.91678205471.507020324273.55182884246-0.007676307591670.2270370286730.0189052861578-0.142830805769-0.0408181760339-0.0184318578741-0.165761698396-0.02101270453860.01443368894350.3305831717040.01184233035270.00476952647490.509448835809-0.08954720532350.49491060495120.350399092453.876451241423.2915569671
72.28769254228-1.04056967951-0.4138767271983.21536461015-0.03388648567693.653335258780.02124725021460.1130769176660.175442559497-0.105957704029-0.112668088240.0770340118266-0.250749999629-0.3965916494950.1314949214490.4453520564770.053017261888-0.004588070775830.543303133347-0.1940681811930.47673526311510.978174761862.556710690938.2042581433
81.46867017379-1.157886292640.07102329690611.737306787471.182346984122.69247926684-0.22505875101-0.196593166140.09379352578010.46714831345-0.1449711005870.3948760935950.0447109295556-0.623532159810.3528224792530.419672517087-0.004656089570150.05427834888880.636689628078-0.1334355599560.5791184441498.9422176650455.368620165141.5261516354
91.36493178264-0.3450157670030.3361945482112.720680199932.297000849997.09771589187-0.122055647959-0.0999082730236-0.2643035773410.713514412450.191162032927-0.2351091914281.00639428720.884273095257-0.06111247740390.4757557602340.00802263367942-0.03854225403670.493859955999-0.04371657755710.54330934830925.457610993337.814666489430.7758114771
102.86225895967-1.39520548684-0.07076212651893.467236432070.08686870957712.78062120404-0.15295610.2900816928920.373089732186-0.1778528268120.02375909402950.140737920632-0.5285817961950.02101302581020.1005189808510.551393861746-0.0860858165594-0.01257673417160.5407130210.005644296916890.52921931250214.358797240842.3233497531-13.0493059065
110.670298678537-0.566208147274-0.1945701001142.83418060590.3292662320232.41330431196-0.03630602712740.191763141471-0.00665143839112-0.2458337674480.134463544872-0.1475622433540.01795967233040.228383880769-0.08877268265520.372779530204-0.06354221513380.03922015512120.524869461729-0.04793704539180.47845235533323.278067937627.4771406772-11.2383075993
121.42024018951-0.8724995368850.5027826261761.307604743130.5941565479032.96932642240.0430976101409-0.01024611351520.08651602968530.0155310015487-0.1475618155130.258786998635-0.0117664685735-0.4749023475320.08555576213370.321938246827-0.05924814998670.03298425546560.444803911618-0.04511252156790.4617305282133.3975311535628.71865897360.30719920102
132.58177592734-0.7756581057451.318451914172.00097839449-0.1149214949832.9548643247-0.0795036081755-0.3300490169950.247309544897-0.0350346405384-0.06748764646760.2480591738680.139645904135-0.3479830311590.08359778673490.406035992292-0.04603567942940.04834279404670.496143012417-0.1124140540950.5778347338480.72405578983634.89326446498.76685097844
141.38425620616-0.920942560358-0.4623039010292.355629594131.487885595672.18842368952-0.08143914763230.02887018192840.03212101768130.3580934543670.111868943184-0.1389978338180.4722553363140.16195012635-0.0356373409840.4492261508-0.01802498334970.006447616773220.37936568137-0.04667236747680.48694934257319.873628172313.31528834482.71082346516
152.69137450516-0.916898710897-0.001785177890092.30494541357-0.1948507308752.57391572683-0.08362036877390.72828068409-0.00275342237127-0.7205294577230.1569487164-0.2581111133120.1754449873290.0105020671607-0.04721332473810.805135586839-0.1656594592570.08177728827430.93669124483-0.1105205727960.46994185005119.96759476876.34683243892-44.1008784869
162.4989194616-0.0337386404676-0.116610286452.28633753639-0.2401988242022.50287577077-0.2496841369410.56579809062-0.312318251808-0.5390764093910.236435729435-0.2680968112890.4311557847250.0981938951268-0.00938401859380.783435323217-0.07471351380450.1153965583740.730153201215-0.2301891850090.53623701457222.6190006731-4.23581154646-35.1382681738
171.9070218196-1.62140719707-0.6346256332293.010875406920.05634500351082.736275088060.01368867184270.449670992992-0.054735434432-0.39928559721-0.06219944622860.2596481354690.0906180585186-0.401190903146-0.03170574891410.581339120271-0.07517723378380.005602647175270.676980027881-0.1415749046370.48618823117610.2690787462.57609422414-24.8626443133
182.36749944009-0.76695996057-0.6821814763481.657249659030.6218321075442.58668031318-0.2888085954790.251862063037-0.4048059649970.01902989038960.132661392970.05183555558660.659554862865-0.07765482598920.1572445987840.695681281656-0.137287435260.08532023114720.513021080266-0.1589373512640.52920463671615.0706131399-7.79215229015-21.924283689
192.07504688037-1.50629686147-0.3473767970742.776389865031.288419868091.41592646913-0.270580870968-0.04534128996150.2772312187970.6349202925420.369365588104-0.3979147242160.1106111693560.454390615497-0.07079994701531.15464881274-0.09964266984540.001991832943250.878945632525-0.1961081083910.78664281039927.620780667592.100807703582.0260894936
200.261342499162-0.443423824193-0.7989732886280.8863180665051.302611775622.03525750858-0.00528494405464-0.0749039803206-0.006816406828920.4820293182260.470155764411-0.5755094927590.6189380284310.643323869765-0.6902007640730.59481644220.1183988793360.01238887389880.938420567976-0.2637490890280.86049045146642.852734105527.59204094444.13763665009
212.832240355580.547848786254-1.589796473592.907657899490.7261404816123.80935334179-0.427820061520.335681921778-0.46067482518-0.09744971655220.162522889105-0.06777221353150.898725238291-0.4025710433570.2229620999210.960516039681-0.08890973502930.09526479591750.63694292798-0.1241114428140.7117651967856.1623843810154.418754224869.6080051774
224.871615033690.82590674058-0.1199779543793.47256031257-3.47225578273.70408721330.220858314168-0.4114367054060.3018964928520.46881434959-0.258716507808-0.602705394665-0.5013263015380.8915936669050.005515709619320.8302926005060.0249572033954-0.004820672686340.8400728754570.01929809978380.70171230505611.345139568765.330003018297.1764682467
233.988021633061.318231652110.2863428630883.69401694822-0.7660317829252.051755402-0.23342450999-0.873746802468-0.4000018052410.513923343365-0.494976076651-1.118301217740.07507700754451.408145908840.7162802230541.059378001810.16180232312-0.1703510886661.696360655260.3593506754291.2746370060521.027355588956.1603638079107.483044741
242.123530071840.915037960321-1.069017892161.66246517796-0.5210031427313.04897835323-0.356404753586-0.354487782631-0.7830057067060.2000785601870.100650846615-0.08030207962351.02172814633-0.0337371805970.1530913966191.136911065930.1240663739220.2288396944950.5332001177370.168280175360.850099612632-0.23955065555850.647719572399.1888880782
250.424497215165-0.068035596069-0.3352424421450.438365305358-0.116016634910.327243652646-0.554133351066-0.24937752949-1.398414340870.649948256912-0.478709483737-0.5749784209040.7925443720810.6515092180330.9230014765712.297390459880.2758585896280.3979846329651.013016968730.1711619615291.61016186044.5137998167237.4409505054101.345893756
262.262476290320.0737662902602-1.82693150530.4219232957370.6866991520822.74264783293-0.376358132617-0.18861686121-0.5038839192520.2045418277250.224178686119-0.01917799970720.7476900237230.06813678404980.1110343452261.002349923820.09195775373560.13285246340.5150748726680.09286244512390.708244504986-1.9771544489357.542692253594.8786182145
274.45003203947-1.24904502519-0.5514414146130.620019011066-1.035062806786.18754672131-0.03532426333080.561111888686-0.308171020524-0.069797403940.2062013520570.3346613345320.590532703978-0.635043752129-0.1879466400610.836822357964-0.2218264056290.09004237525530.660167121767-0.03412818675010.774273542913-6.8026938375257.460817590580.06051368
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 71 )AA1 - 711 - 71
22chain 'A' and (resid 72 through 273 )AA72 - 27372 - 273
33chain 'A' and (resid 274 through 311 )AA274 - 311274 - 311
44chain 'A' and (resid 312 through 438 )AA312 - 438312 - 438
55chain 'B' and (resid 1 through 88 )BF1 - 881 - 86
66chain 'B' and (resid 89 through 238 )BF89 - 23887 - 236
77chain 'B' and (resid 239 through 295 )BF239 - 295237 - 289
88chain 'B' and (resid 296 through 401 )BF296 - 401290 - 387
99chain 'B' and (resid 402 through 438 )BF402 - 438388 - 424
1010chain 'C' and (resid 1 through 72 )CL1 - 721 - 72
1111chain 'C' and (resid 73 through 199 )CL73 - 19973 - 199
1212chain 'C' and (resid 200 through 311 )CL200 - 311200 - 311
1313chain 'C' and (resid 312 through 372 )CL312 - 372312 - 372
1414chain 'C' and (resid 373 through 440 )CL373 - 440373 - 440
1515chain 'D' and (resid 1 through 109 )DP1 - 1091 - 107
1616chain 'D' and (resid 110 through 238 )DP110 - 238108 - 236
1717chain 'D' and (resid 239 through 295 )DP239 - 295237 - 288
1818chain 'D' and (resid 296 through 441 )DP296 - 441289 - 426
1919chain 'E' and (resid 6 through 46 )ET6 - 461 - 26
2020chain 'E' and (resid 47 through 143 )ET47 - 14327 - 123
2121chain 'F' and (resid 1 through 66 )FU1 - 661 - 66
2222chain 'F' and (resid 67 through 96 )FU67 - 9667 - 96
2323chain 'F' and (resid 97 through 184 )FU97 - 18497 - 160
2424chain 'F' and (resid 185 through 236 )FU185 - 236161 - 209
2525chain 'F' and (resid 237 through 257 )FU237 - 257210 - 230
2626chain 'F' and (resid 258 through 339 )FU258 - 339231 - 312
2727chain 'F' and (resid 340 through 382 )FU340 - 382313 - 345

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