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- PDB-9h34: T2R-TTL-PROTAC1 complex -

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Basic information

Entry
Database: PDB / ID: 9h34
TitleT2R-TTL-PROTAC1 complex
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
  • Tubulin--tyrosine ligase
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE
Function / homology
Function and homology information


tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / cellular response to interleukin-4 / tubulin binding ...tubulin-tyrosine ligase / regulation of metaphase plate congression / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / regulation of microtubule polymerization or depolymerization / microtubule-based process / cytoplasmic microtubule / cellular response to interleukin-4 / tubulin binding / positive regulation of mitotic cell cycle / post-translational protein modification / spindle microtubule / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration / neuron projection development / mitotic cell cycle / double-stranded RNA binding / microtubule cytoskeleton / growth cone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / neuron projection / cilium / protein heterodimerization activity / GTPase activity / ubiquitin protein ligase binding / GTP binding / Golgi apparatus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
: / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. ...: / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
: / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Tubulin--tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain / Tubulin beta-2B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Bos taurus (domestic cattle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.3 Å
AuthorsMaiocchi, A. / Abel, A.-C. / Steinmetz, M.O. / Passarella, D. / Prota, A.E.
Funding supportEuropean Union, Switzerland, 2items
OrganizationGrant numberCountry
European Union (EU)860070 TubInTrainEuropean Union
Swiss National Science Foundation31003A_166608 Switzerland
CitationJournal: To Be Published
Title: Effective tubulin degradation by rationally designed Proteolysis Targeting Chimeras
Authors: Maiocchi, A. / Abel, A.-C. / Basellini, M. / Perez Pena, H. / Boiarska, Z. / Ferrandi, E. / Kozicka, Z. / Fasano, V. / Pieraccini, S. / Cappelletti, G. / Steinmetz, M.O. / Prota, A.E. / Passarella, D.
History
DepositionOct 15, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 29, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta-2B chain
C: Tubulin alpha-1B chain
D: Tubulin beta-2B chain
E: Stathmin-4
F: Tubulin--tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)265,86824
Polymers261,6316
Non-polymers4,23718
Water3,513195
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: equilibrium centrifugation
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)104.370, 156.420, 181.930
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 50204.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Brain / References: UniProt: P81947
#2: Protein Tubulin beta-2B chain


Mass: 49999.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: brain / References: UniProt: Q6B856
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16844.162 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli (E. coli) / References: UniProt: P63043
#4: Protein Tubulin--tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli (E. coli) / References: UniProt: A0A8V0Z8P0, tubulin-tyrosine ligase

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Non-polymers , 9 types, 213 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Mg
#7: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#9: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#10: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O3
#11: Chemical ChemComp-A1ISB / (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl 4-{2-[4-({2-[2-({2-[(3R)-2,6-dioxopiperidin-3-yl]-1,3-dioxo-2,3-dihydro-1H-isoindol-4-yl}amino)ethoxy]ethoxy}methyl)-1H-1,2,3-triazol-1-yl]ethyl}benzoate


Mass: 1137.624 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C57H65ClN8O15 / Feature type: SUBJECT OF INVESTIGATION
#12: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 195 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.84 Å3/Da / Density % sol: 56.66 %
Crystal growTemperature: 293 K / Method: vapor diffusion
Details: 4% PEG 4K, 13% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1.00003 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 21, 2023
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.00003 Å / Relative weight: 1
ReflectionResolution: 2.3→49.72 Å / Num. obs: 132501 / % possible obs: 99.98 % / Redundancy: 54.1 % / Biso Wilson estimate: 67.58 Å2 / CC star: 1 / Rmerge(I) obs: 0.1021 / Rpim(I) all: 0.01401 / Rrim(I) all: 0.1031 / Net I/σ(I): 29.63
Reflection shellResolution: 2.3→2.33 Å / Rmerge(I) obs: 4.39 / Mean I/σ(I) obs: 0.98 / Num. unique obs: 4409 / CC1/2: 0.508 / Rpim(I) all: 0.5898 / Rrim(I) all: 4.43

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Processing

Software
NameVersionClassification
PHENIX1.21.1_5286refinement
XDSdata reduction
XSCALEdata scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 2.3→49.72 Å / SU ML: 0.3563 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.7476
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2515 6626 5 %
Rwork0.2328 125856 -
obs0.2337 132482 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 89.62 Å2
Refinement stepCycle: LAST / Resolution: 2.3→49.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17356 0 265 195 17816
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.001518019
X-RAY DIFFRACTIONf_angle_d0.43124427
X-RAY DIFFRACTIONf_chiral_restr0.03912657
X-RAY DIFFRACTIONf_plane_restr0.00263156
X-RAY DIFFRACTIONf_dihedral_angle_d11.21436677
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.330.36842210.37124186X-RAY DIFFRACTION99.86
2.33-2.350.35512140.34724139X-RAY DIFFRACTION99.98
2.35-2.380.36952200.34894146X-RAY DIFFRACTION99.95
2.38-2.410.36522210.34684149X-RAY DIFFRACTION100
2.41-2.440.37012180.34114163X-RAY DIFFRACTION99.95
2.44-2.480.36042200.36274146X-RAY DIFFRACTION100
2.48-2.510.34412160.3574143X-RAY DIFFRACTION100
2.51-2.550.38212190.34524136X-RAY DIFFRACTION99.98
2.55-2.590.29352190.32044169X-RAY DIFFRACTION100
2.59-2.630.36472190.31934155X-RAY DIFFRACTION99.98
2.63-2.680.31742200.31474173X-RAY DIFFRACTION100
2.68-2.730.32062190.31254181X-RAY DIFFRACTION100
2.73-2.780.28442180.31114126X-RAY DIFFRACTION99.98
2.78-2.840.37032190.30424189X-RAY DIFFRACTION99.95
2.84-2.90.32142220.30514190X-RAY DIFFRACTION100
2.9-2.970.30892200.3044166X-RAY DIFFRACTION100
2.97-3.040.32672180.31344179X-RAY DIFFRACTION100
3.04-3.120.33122200.30534160X-RAY DIFFRACTION100
3.12-3.210.31592220.29414182X-RAY DIFFRACTION100
3.21-3.320.30612180.27034198X-RAY DIFFRACTION100
3.32-3.440.29412220.27184189X-RAY DIFFRACTION100
3.44-3.570.29162220.24664192X-RAY DIFFRACTION100
3.57-3.740.24242210.23234228X-RAY DIFFRACTION100
3.74-3.930.24292200.21734191X-RAY DIFFRACTION100
3.93-4.180.23112220.2064235X-RAY DIFFRACTION100
4.18-4.50.1942240.19054216X-RAY DIFFRACTION100
4.5-4.950.21972240.1824267X-RAY DIFFRACTION100
4.95-5.670.24272240.20894267X-RAY DIFFRACTION100
5.67-7.140.21512270.224304X-RAY DIFFRACTION100
7.14-49.720.1812370.17024491X-RAY DIFFRACTION99.77
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.6952600723-1.09494523973-0.8073070747914.91599178088-0.4011205334721.515907015630.01128165579520.02669455572130.437223857475-0.7054753750610.286073036984-0.391843823384-1.130532574640.424709977213-0.2193369141121.04390367444-0.1575550872680.1301550241350.724967178287-0.1710270745050.61559826867528.878584630896.867220939554.3679497969
21.81610466866-2.130206217491.958962840079.753516231741.614113752325.34468286809-0.2108273510740.5605949136110.0741598155799-1.427958068410.304953158369-0.0815720228305-1.322906690930.472977165479-0.1113071807090.993818930698-0.1884942984910.1351278017440.774771963835-0.1327018213320.78567817838428.149831342286.658767600742.8404982188
32.205558312550.227922450969-0.2190359874057.228019265533.181733270334.094880602560.05978534696180.2116192841860.0548140099717-0.351820993460.384105962512-0.873097802234-0.661121935060.775979738615-0.449696796240.633783114704-0.1415402105060.08958604502120.956225168825-0.2989175290870.80991216876638.406185154480.521261855552.5754141315
41.974129131770.350129952896-1.315623427167.96641081071.327251446083.963508424560.1099974748930.0478938868078-0.1542619277190.8719788041620.282681491221-0.3330950340160.2132302295541.03631477553-0.4180316551940.5937542130630.0565945421163-0.01765520066790.636118234065-0.1945631581440.53117281371430.012269765373.385802618659.4053509041
52.405429064790.1346196275530.9002152761533.69570122290.4712512236655.465812385270.126712134324-0.09734419660510.1502430679240.346778116817-0.006189159927880.286112352481-0.195630805527-0.11096411873-0.115366352370.6030778824920.03643393847330.1005240217540.519172714054-0.1312214510120.60724223989917.369776344883.448385477467.4472574198
61.39573822528-0.5782969671790.03958936004594.82918758542.494893264434.42493863755-0.0837720754962-0.2602551763260.0772842582940.88103616120.25894814408-0.03412743049240.3222843527530.32158072382-0.1860675033840.548845257064-0.0403920136660.02863212669310.590834254508-0.09780242758340.60715094270122.857838831675.976424963270.207403651
73.06003713274-1.2937939275-0.05407187432821.42262431503-1.421862753512.090350195420.1378742703130.1553370305380.773458928422-0.326738464735-0.1829903071630.193542703612-0.899387338629-0.3038378732050.099607244810.6786696999340.04006265468130.03064217639720.56414334114-0.05457321674290.7522312987516.095263556669.571141271119.4646412215
81.79049374983-0.431402515571-0.04165940338034.130761111321.999646164593.86080994457-0.02914099602190.2300314355110.0999236839122-0.01581360878170.0969096853394-0.067110665616-0.1915533144910.120413422963-0.004445338440.3829029515670.02880073255630.01683035491750.573439953709-0.094455823490.55084084065423.255074556853.333992836823.2896003559
91.86034171827-1.221252386120.2624100466452.243730399210.8805614573233.95345166620.123008860025-0.3010030576730.2386535946120.0213591518383-0.3835490996560.313082207478-0.313115856703-0.6987047652950.09213937291890.3958675130410.05823624134180.05875215506320.564339775583-0.1469950181690.65624417639811.23472094861.717672150931.7348875007
103.19114796994-0.349285868949-0.4872648526162.43800022919-0.3349475363.57914292755-0.265645064553-0.3288045006850.06656091657530.238540637060.1216790072660.599546535719-0.041565889776-1.023972147450.01361555348050.5338364815290.0135834372819-0.0004255242356240.843098814218-0.2363443287370.64687685795.1661462479258.908982533542.1502524063
110.85480344353-0.391933312347-0.1666468625791.882556018541.786559203353.87936836485-0.14007700321-0.2654061054930.0140804574580.627450831790.1109723963780.05692431276920.550527894004-0.03250851493150.04752405019720.448880206086-0.0738422930440.01662866229050.554953110356-0.07522890003120.58135637520917.542945684745.812500725935.6974546302
121.77178627453-1.03093943390.2048148328664.12541879896-0.2913684835921.60893452083-0.04267821261270.2766121567170.1708732914-0.2839468388470.108491400813-0.189196812468-0.193323445290.21021054668-0.04760121022010.454524227414-0.1026204839570.03388751816390.620301955559-0.04510119428120.49073500148920.189977161632.6390391134-12.054257033
132.17576124003-1.094311141651.399468601991.224181585010.7272392900743.581078613520.0303874527571-0.07338370080950.07076223326390.0970243732684-0.1349359200540.3309700852990.0476669435213-0.5046595783150.01525559101820.38636324502-0.06569598375740.05007384594690.535214078325-0.05179154289370.5248015997193.835745315328.48029684760.337536099796
141.405941764720.273693357904-0.3400022001861.42308006477-0.2108129282680.774635020934-0.146153424922-0.3699693194870.03664096869750.315170677312-0.03033340903240.2578030659490.39017603114-0.555053466697-0.4204202712920.498288431267-0.08398841835520.06178425590370.615859928156-0.1246621552260.721424345277-0.60987441250435.851269818111.9451651824
151.77586589138-1.53531559583-0.6078363164492.525785284181.255304971.78399929072-0.087540603097-0.2169437038570.0251570218865-0.01717134406440.0599997265040.3845211571950.130403451092-0.1448944351070.1422038894350.515775357513-0.151730397844-0.005792253823960.545429732936-0.1100557352180.5840837069956.7407974340625.74597724735.02138753596
162.26856026599-0.3958648056630.6631342659572.003459317280.4060476219940.806397448475-0.0538197254601-0.185572296705-0.04585493549040.5487516883390.24321691166-0.3580876692160.6609546609430.5633630932050.1825957893970.5676057624910.0296834533562-0.0455033030750.427240181804-0.08388619830730.59441305257925.134893350810.74459195562.23169002684
171.68767933537-0.10630318695-0.266424428210.352780311907-0.8723531450940.840880753048-0.494888111921.069088905710.1569860582590.002485995577970.931021558382-0.2460007507570.163588874402-0.514408098623-0.1523353568780.89192242138-0.2295812714150.04314485901111.22948198299-0.161873521550.5650413630617.66579661824.4620900411-40.4639625154
181.513668588620.3925591007112.842353008060.7940596499952.33653938517.18397316381-0.1903093062760.5781915857530.885769455132-0.2052718319040.432044097481-0.319072542868-0.789828714310.33297885665-0.7514557011150.901707969317-0.06315806540890.1271481325881.144751085460.0450128251870.76347045447113.734155922717.167822105-42.6120474821
193.30826460969-0.991436210826-1.304960697935.791456039312.035699359891.40252442068-0.2367462553681.758591905910.438579203132-1.771035329470.1797685058320.3306038230570.573016225499-0.5595308273140.3136847162141.30068862037-0.3457381174410.1722774269271.50603097513-0.2191784607250.69066258539321.92943636971.50635732044-50.4547889977
202.98646496323-0.388503278067-0.9892829405193.73736478252-1.343542577341.91078101916-0.06933653826070.7853197363360.0250954479529-0.861952544440.014304586928-0.509185204761-0.01454234592570.2819825643730.2093669829130.895824275263-0.08650053781290.2086385448151.10309995708-0.3041509200610.6925766446331.9248494753-0.0971165777341-39.925237777
211.62425894663-1.28161990941-0.4128093170740.242039067769-1.066821613751.93203909696-0.03141834351670.0663117632953-0.482048522577-0.390262236743-0.0534873494906-0.03203627799730.671993022672-0.329862983440.1439850065071.02018319425-0.1464600973270.09555105003250.895621645922-0.2828316050570.77347888547821.2171942086-9.5311491136-31.9198768568
221.92276270339-1.6491982325-0.03208385778312.627606335170.6413643792832.5893738536-0.1673978890390.30714696502-0.323360573336-0.3535090549370.002598361471140.5504201505260.235172812037-0.637709646381-0.09864685771370.693281190332-0.156500321621-0.002027375814690.722990346918-0.1818864785060.5374453269527.25646250422-0.89861201049-25.4240768183
232.8112151874-0.181959090877-0.1761887842471.192338601230.08156912990052.54076796265-0.2340081410970.389114251129-0.698808996292-0.1601821491050.0548354127986-0.04425520944990.6494395964710.02462508783890.179527381790.988365393475-0.09084295739040.1455487505430.635814891856-0.2044542094240.73805322358420.5287756657-11.3428413153-23.2224496559
240.884213255418-0.3708015820040.2015808627261.981928425450.2285692618510.239550971926-0.306921399639-0.4931599900260.1003791271240.74589506190.412872838717-0.7748788622610.2037574456470.473705512797-0.02139582895631.24309301524-0.0928521504452-0.05027416206491.16601087238-0.213104282830.97770471765327.392205994291.735559797582.6130561231
250.427274486108-0.581396488861-0.9329679005980.0655511888216-0.08042716072340.790392785073-0.0582732662172-0.12996445814-0.06536169415010.2050024790130.634373734484-0.6373398945430.3778803342940.918021327083-0.09822041849020.5382420065370.09614316163160.03480309688810.992279087669-0.2798209333990.92317402723843.009913855127.36410974694.25272014629
262.474676832380.329605460134-3.239363199932.03112276498-2.309602180682.68661462644-0.239106753217-0.419212133418-0.4697703774770.09278916418350.0401787942176-0.4927176595020.7363281757720.6780705467350.5781372399650.8398589803680.1353470525610.01860044826170.641263449333-0.02793002407890.82360345162710.891218148357.879597804584.8169809088
271.737321025470.611791967616-1.42520942741.845484679580.1339264611372.02884853344-0.432702653457-0.824272045498-1.194483277770.4428077905950.186294180357-0.5108286475850.8932050807530.788469993495-0.2741121805911.268285202180.1915510611920.1418824185441.024408910750.2881546527021.072600152337.9621486473152.0131354845101.83132083
280.6210923879611.36736301413-1.230202489980.6943745615130.5436384316271.25028281802-0.239420658408-0.287079541045-0.7852728355070.5460048758330.0665913094938-0.362040774130.8586147802750.3699902807390.334485266051.473401732870.1160069438890.2353714169870.7661866972090.2330579265351.0952840459-1.7414030151548.2764300703101.079845484
292.18612877915-0.3625278271420.577714761981.667445536123.263693687511.92529455378-0.539142250166-0.546448005839-0.2926120709961.31118594435-0.964890181833-2.34431437505-1.455498082511.914876953820.008868747307430.7408914971160.3498563853950.3908481726160.129659168125-0.1465216496860.1747290309-1.5310671946657.813867119786.1826527445
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 71 )AA1 - 711 - 71
22chain 'A' and (resid 72 through 102 )AA72 - 10272 - 102
33chain 'A' and (resid 103 through 160 )AA103 - 160103 - 160
44chain 'A' and (resid 161 through 199 )AA161 - 199161 - 199
55chain 'A' and (resid 200 through 311 )AA200 - 311200 - 309
66chain 'A' and (resid 312 through 438 )AA312 - 438310 - 436
77chain 'B' and (resid 1 through 88 )BF1 - 881 - 86
88chain 'B' and (resid 89 through 215 )BF89 - 21587 - 213
99chain 'B' and (resid 216 through 259 )BF216 - 259214 - 257
1010chain 'B' and (resid 260 through 338 )BF260 - 338258 - 332
1111chain 'B' and (resid 339 through 437 )BF339 - 437333 - 423
1212chain 'C' and (resid 1 through 197 )CL1 - 1971 - 197
1313chain 'C' and (resid 198 through 311 )CL198 - 311198 - 311
1414chain 'C' and (resid 312 through 337 )CL312 - 337312 - 337
1515chain 'C' and (resid 338 through 400 )CL338 - 400338 - 400
1616chain 'C' and (resid 401 through 440 )CL401 - 440401 - 440
1717chain 'D' and (resid 1 through 27 )DQ1 - 271 - 27
1818chain 'D' and (resid 28 through 64 )DQ28 - 6428 - 62
1919chain 'D' and (resid 65 through 88 )DQ65 - 8863 - 86
2020chain 'D' and (resid 89 through 160 )DQ89 - 16087 - 158
2121chain 'D' and (resid 161 through 215 )DQ161 - 215159 - 213
2222chain 'D' and (resid 216 through 338 )DQ216 - 338214 - 331
2323chain 'D' and (resid 339 through 441 )DQ339 - 441332 - 426
2424chain 'E' and (resid 6 through 46 )EU6 - 461 - 26
2525chain 'E' and (resid 47 through 143 )EU47 - 14327 - 123
2626chain 'F' and (resid 1 through 140 )FV1 - 1401 - 121
2727chain 'F' and (resid 141 through 223 )FV141 - 223122 - 199
2828chain 'F' and (resid 224 through 302 )FV224 - 302200 - 275
2929chain 'F' and (resid 303 through 382 )FV303 - 382276 - 345

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