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- PDB-9bol: Crystal structure of the complex between VHL, ElonginB, ElonginC,... -

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Basic information

Entry
Database: PDB / ID: 9bol
TitleCrystal structure of the complex between VHL, ElonginB, ElonginC, and compound 5
Components
  • Elongin-B
  • Elongin-C
  • von Hippel-Lindau disease tumor suppressor
KeywordsTRANSCRIPTION / VHL / degradation / degrader
Function / homology
Function and homology information


regulation of cellular response to hypoxia / RHOBTB3 ATPase cycle / negative regulation of receptor signaling pathway via JAK-STAT / target-directed miRNA degradation / transcription elongation factor activity / VCB complex / elongin complex / Replication of the SARS-CoV-1 genome / Cul5-RING ubiquitin ligase complex / intracellular membraneless organelle ...regulation of cellular response to hypoxia / RHOBTB3 ATPase cycle / negative regulation of receptor signaling pathway via JAK-STAT / target-directed miRNA degradation / transcription elongation factor activity / VCB complex / elongin complex / Replication of the SARS-CoV-1 genome / Cul5-RING ubiquitin ligase complex / intracellular membraneless organelle / Cul2-RING ubiquitin ligase complex / SUMOylation of ubiquitinylation proteins / negative regulation of transcription elongation by RNA polymerase II / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / Tat-mediated elongation of the HIV-1 transcript / ubiquitin-like ligase-substrate adaptor activity / Formation of HIV-1 elongation complex containing HIV-1 Tat / negative regulation of signal transduction / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / negative regulation of TORC1 signaling / protein serine/threonine kinase binding / RNA Polymerase II Pre-transcription Events / negative regulation of autophagy / transcription corepressor binding / positive regulation of cell differentiation / TP53 Regulates Transcription of DNA Repair Genes / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / Vif-mediated degradation of APOBEC3G / Inactivation of CSF3 (G-CSF) signaling / cell morphogenesis / Evasion by RSV of host interferon responses / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Regulation of expression of SLITs and ROBOs / ubiquitin-protein transferase activity / transcription corepressor activity / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Neddylation / microtubule cytoskeleton / regulation of gene expression / Replication of the SARS-CoV-2 genome / protein-containing complex assembly / ubiquitin-dependent protein catabolic process / protein-macromolecule adaptor activity / DNA-binding transcription factor binding / cellular response to hypoxia / molecular adaptor activity / amyloid fibril formation / proteasome-mediated ubiquitin-dependent protein catabolic process / protein stabilization / protein ubiquitination / cilium / negative regulation of cell population proliferation / negative regulation of gene expression / ubiquitin protein ligase binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / enzyme binding / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / mitochondrion / proteolysis / nucleoplasm / nucleus / plasma membrane / cytosol
Similarity search - Function
von Hippel-Lindau disease tumour suppressor, beta/alpha domain / von Hippel-Lindau disease tumour suppressor, alpha domain / von Hippel-Lindau disease tumour suppressor, beta domain / VHL superfamily / von Hippel-Lindau disease tumour suppressor, alpha domain superfamily / von Hippel-Lindau disease tumour suppressor, beta domain superfamily / VHL beta domain / VHL box domain / Elongin-C / Elongin B ...von Hippel-Lindau disease tumour suppressor, beta/alpha domain / von Hippel-Lindau disease tumour suppressor, alpha domain / von Hippel-Lindau disease tumour suppressor, beta domain / VHL superfamily / von Hippel-Lindau disease tumour suppressor, alpha domain superfamily / von Hippel-Lindau disease tumour suppressor, beta domain superfamily / VHL beta domain / VHL box domain / Elongin-C / Elongin B / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
: / von Hippel-Lindau disease tumor suppressor / Elongin-C / Elongin-B
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.99 Å
AuthorsMurray, J.M. / Wu, H. / Fuhrmann, J. / Fairbrother, W.J. / DiPasquale, A.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: J.Med.Chem. / Year: 2024
Title: Potency-Enhanced Peptidomimetic VHL Ligands with Improved Oral Bioavailability.
Authors: Wu, H. / Murray, J. / Ishisoko, N. / Frommlet, A. / Deshmukh, G. / DiPasquale, A. / Mulvihill, M.M. / Zhang, D. / Quinn, J.G. / Blake, R.A. / Fairbrother, W.J. / Fuhrmann, J.
History
DepositionMay 3, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 29, 2024Provider: repository / Type: Initial release
Revision 1.1Oct 16, 2024Group: Database references / Structure summary / Category: citation / citation_author / pdbx_entry_details
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Elongin-B
B: Elongin-C
C: von Hippel-Lindau disease tumor suppressor
D: Elongin-B
E: Elongin-C
F: von Hippel-Lindau disease tumor suppressor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)87,79517
Polymers86,2126
Non-polymers1,58311
Water6,287349
1
A: Elongin-B
B: Elongin-C
C: von Hippel-Lindau disease tumor suppressor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)43,9299
Polymers43,1063
Non-polymers8226
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6310 Å2
ΔGint-42 kcal/mol
Surface area17170 Å2
MethodPISA
2
D: Elongin-B
E: Elongin-C
F: von Hippel-Lindau disease tumor suppressor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)43,8668
Polymers43,1063
Non-polymers7605
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6360 Å2
ΔGint-40 kcal/mol
Surface area16480 Å2
MethodPISA
Unit cell
Length a, b, c (Å)47.460, 47.740, 100.350
Angle α, β, γ (deg.)103.62, 94.66, 98.33
Int Tables number1
Space group name H-MP1

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Components

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Protein , 3 types, 6 molecules ADBECF

#1: Protein Elongin-B / EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / ...EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / Transcription elongation factor B polypeptide 2


Mass: 11748.406 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOB, TCEB2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q15370
#2: Protein Elongin-C / EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / ...EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / Transcription elongation factor B polypeptide 1


Mass: 10843.420 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOC, TCEB1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q15369
#3: Protein von Hippel-Lindau disease tumor suppressor / Protein G7 / pVHL


Mass: 20514.275 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: VHL / Production host: Escherichia coli (E. coli) / References: UniProt: P40337

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Non-polymers , 5 types, 360 molecules

#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical ChemComp-DMS / DIMETHYL SULFOXIDE


Mass: 78.133 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6OS / Comment: DMSO, precipitant*YM
#6: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#7: Chemical ChemComp-A1ARP / (4R)-1-[(2S)-2-(4-cyclopropyl-1H-1,2,3-triazol-1-yl)-3,3-dimethylbutanoyl]-4-hydroxy-N-{[4-(4-methyl-1,3-thiazol-5-yl)phenyl]methyl}-L-prolinamide


Mass: 522.662 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C27H34N6O3S / Feature type: SUBJECT OF INVESTIGATION
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 349 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.52 Å3/Da / Density % sol: 51.15 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop
Details: 0.1 mM sodium cacodylate pH 5.7, 15% PEG8000, 0.2 M magnesium acetate, 5 mM DTT

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Data collection

DiffractionMean temperature: 93 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 13, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 1.99→46.66 Å / Num. obs: 52695 / % possible obs: 91.4 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.048 / Net I/σ(I): 12.6
Reflection shellResolution: 1.99→2.04 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.53 / Num. unique obs: 3923 / CC1/2: 0.771

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Processing

Software
NameVersionClassification
REFMAC5.8.0238refinement
XDSdata reduction
STARANISOdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.99→46.66 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.935 / SU B: 10.95 / SU ML: 0.153 / Cross valid method: THROUGHOUT / ESU R: 0.211 / ESU R Free: 0.184 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.24791 2487 4.9 %RANDOM
Rwork0.20171 ---
obs0.20394 48292 88.05 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 42.831 Å2
Baniso -1Baniso -2Baniso -3
1--1.19 Å2-0.13 Å2-1.33 Å2
2--2.57 Å2-0.1 Å2
3----0.93 Å2
Refinement stepCycle: 1 / Resolution: 1.99→46.66 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5467 0 104 349 5920
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0030.0135749
X-RAY DIFFRACTIONr_bond_other_d0.0010.0175402
X-RAY DIFFRACTIONr_angle_refined_deg1.2241.687809
X-RAY DIFFRACTIONr_angle_other_deg1.0871.5912534
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.675683
X-RAY DIFFRACTIONr_dihedral_angle_2_deg33.48621.195318
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.08915979
X-RAY DIFFRACTIONr_dihedral_angle_4_deg18.7521551
X-RAY DIFFRACTIONr_chiral_restr0.0440.2745
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.026326
X-RAY DIFFRACTIONr_gen_planes_other0.0010.021211
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.472.5582738
X-RAY DIFFRACTIONr_mcbond_other0.472.5582737
X-RAY DIFFRACTIONr_mcangle_it0.8053.8323419
X-RAY DIFFRACTIONr_mcangle_other0.8053.8323420
X-RAY DIFFRACTIONr_scbond_it0.4672.6653011
X-RAY DIFFRACTIONr_scbond_other0.4672.6653012
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other0.7913.9554385
X-RAY DIFFRACTIONr_long_range_B_refined4.530.3656091
X-RAY DIFFRACTIONr_long_range_B_other4.530.3676092
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 1.99→2.042 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.299 179 -
Rwork0.303 3597 -
obs--88.95 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.4360.3615-0.17377.5312-2.7936.83330.19890.2595-0.4756-0.763-0.2810.48790.0302-0.29270.08210.23690.1179-0.19860.216-0.13850.2002-22.3958-7.5343-36.3661
23.72185.131-0.68497.31670.07595.21370.0502-0.01150.3896-0.0192-0.14120.6699-0.1163-0.2270.0910.17460.1381-0.20970.268-0.12320.3268-24.8964-5.9107-36.5843
32.7548-1.7507-1.63058.40855.99514.9407-0.1335-0.06730.0213-0.30120.03110.4116-0.0191-0.18750.10230.27290.021-0.22780.3666-0.05040.3612-32.4463-5.1912-40.803
45.21680.39491.97936.1019-1.67914.32130.29150.43730.0844-0.8791-0.20251.22380.026-0.1167-0.0890.48550.1226-0.33610.4172-0.05520.3561-31.1836-0.7308-46.3628
52.5634-0.51830.64043.5623-4.82716.5804-0.11510.31430.2126-0.53270.0849-0.07210.6163-0.1520.03030.62360.0824-0.14250.4105-0.16160.3965-23.519-12.5421-47.5651
60.96171.05291.52676.74153.163212.138-0.01420.2332-0.3228-0.5336-0.50320.39130.32220.1160.51750.18350.0637-0.0670.2321-0.150.3151-16.9814-14.3072-34.2827
74.29061.4371-0.64781.45390.7341.0284-0.22950.99340.4344-0.47570.19030.2394-0.332-0.30810.03920.56690.1298-0.23220.5646-0.13090.4081-27.00671.0841-48.0492
89.3826-0.67023.45180.0568-0.34182.7023-0.09480.3529-0.02650.0067-0.0084-0.0004-0.11270.24150.10320.30310.0055-0.02420.270.00620.2346-22.88292.3186-50.9729
910.44060.60478.00363.1249-0.5666.5086-0.10270.60180.2476-0.4311-0.1119-0.27240.04180.48430.21460.26490.09790.06560.26610.03540.2115-6.78793.9301-35.6914
101.16821.71790.5642.8215-0.915310.6005-0.0952-0.02430.0148-0.1136-0.0271-0.0045-0.1957-0.00830.12230.20860.00760.00310.23480.00350.30477.14352.5422-24.5997
119.93841.317-3.5937.6081-2.81664.0637-0.0770.08130.4915-0.8522-0.12380.92590.3063-0.29890.20080.13250.0472-0.09560.1374-0.10010.3324-23.09346.0294-29.8639
124.43230.3070.14395.9333-2.865311.730.15320.2399-0.4394-0.1092-0.02950.8270.225-0.3306-0.12370.07340.0322-0.0790.1142-0.07830.2303-25.52520.3241-25.4151
136.3981-2.93543.25957.49632.87866.5305-0.1129-0.10970.2986-0.0918-0.0130.3639-0.1988-0.26430.12590.1384-0.00270.01540.1622-0.00750.2064-23.62126.2325-17.3662
1411.8028-0.904-3.61114.791-0.90412.10190.19790.060.788-0.1015-0.01930.5602-0.3129-0.3388-0.17860.31480.0721-0.03850.2163-0.07790.2481-21.256911.5444-25.1307
150.51571.1929-0.615910.0252-1.73342.7940.09190.1633-0.1061-0.3401-0.14990.3740.0222-0.08230.05790.070.0587-0.07630.1391-0.0490.1-16.7312-4.4598-25.0308
167.01861.4198-4.56139.0727-2.36168.7481-0.0086-0.0803-0.3478-0.2148-0.24920.72410.6125-0.2990.25780.11320.0022-0.02480.0736-0.04110.1057-13.9854-13.8322-21.6599
177.211-3.6052-4.79774.32783.09445.09540.06720.0132-0.0234-0.0479-0.0787-0.1137-0.12210.08710.01150.1314-0.0017-0.04440.11450.0380.1172-8.08552.5501-21.2214
182.3381-0.01450.5315.2419-3.12894.69370.0015-0.20840.01360.1699-0.07220.1761-0.1075-0.12350.07070.074-0.0105-0.01760.0259-0.00850.0247-6.411-26.7049-5.5246
191.55050.0336-0.15445.90660.13491.34080.1177-0.0480.24310.3583-0.1089-0.2729-0.08840.188-0.00880.107-0.018-0.03880.07980.01880.0813-3.7629-7.5992-13.6573
2011.13560.8233-7.38742.6842-2.59048.9730.1468-0.49710.37350.11970.16440.4609-0.1285-0.6746-0.31110.465-0.00890.03260.3657-0.06080.3262-11.4037-13.99493.0607
212.49691.7119-1.76743.9607-1.25584.0548-0.1014-0.2542-0.24140.0296-0.0154-0.09970.26490.53520.11680.2010.09290.00480.27290.03580.0357-3.0867-25.4814-44.1078
227.86141.1560.977212.24274.2393.6544-0.0236-0.1980.3490.4-0.06450.1315-0.08580.09820.08810.22610.07550.04230.2756-0.00380.0858-10.156-20.0537-36.2142
233.9918-0.05012.07941.0199-0.98127.2904-0.03570.0860.00730.13890.0930.1264-0.3031-0.0364-0.05730.20370.03410.00930.16920.0070.1755-16.1368-22.322-44.0976
243.03434.9678-0.65668.8088-3.985912.6945-0.0320.03910.19060.10190.24060.3475-0.5698-0.833-0.20860.19830.0445-0.03210.22710.00770.0874-12.801-17.1496-54.2488
2512.4166-0.51294.50750.1118-0.20591.67320.17480.089-0.03680.0626-0.16610.02980.13810.1181-0.00870.31070.0190.0210.3626-0.03520.1215-1.7649-19.8008-42.0232
268.0133-3.85340.18358.6606-0.0750.0104-0.15110.083-0.12410.29480.1935-0.1848-0.0208-0.0092-0.04240.29480.00650.00650.30580.00560.31857.9353-23.7479-29.5567
271.048-3.08271.09079.4237-4.69297.5008-0.1042-0.04-0.01450.28940.05150.0158-0.17760.12820.05280.2224-0.011-0.00030.2773-0.03270.16732.6771-16.7102-34.9742
284.84941.35030.71337.8011-6.785212.5769-0.5192-0.560.23380.18780.001-1.0653-0.0607-0.14690.51820.28450.013-0.08640.3524-0.10170.2472.4841-9.9984-48.0501
295.0748-6.15268.53818.1427-10.762814.631-0.2748-0.26250.29250.47190.06010.1278-0.6397-0.27910.21460.4793-0.0410.0450.3836-0.07820.4152-1.10283.7198-63.6383
301.00550.9501-0.83469.6231-1.3372.3642-0.0399-0.1291-0.02190.0667-0.0093-0.09850.15150.14630.04920.11020.0147-0.00960.2441-0.00490.13786.5914-21.1902-55.4484
316.79042.66693.70655.6014-0.105710.89180.1315-0.3624-0.54830.05930.0448-0.11260.8285-0.059-0.17630.13710.02980.0210.23930.04910.07041.3444-25.6645-58.8463
329.2656-1.1597-0.98893.4654-5.01688.3432-0.0896-0.4006-0.2836-0.1717-0.1395-0.25790.33760.22150.22910.2067-0.0098-0.00460.21810.05030.31876.3276-24.4516-68.2079
335.51560.34240.14328.53542.53783.6889-0.1263-0.2721-0.4796-0.1130.2811-1.10280.4320.4972-0.15480.2348-0.00690.02950.4055-0.02090.197310.7689-19.8165-61.4489
3411.13816.9242.42455.97850.93654.0024-0.023-0.1247-0.07450.0789-0.03510.08070.12220.13430.05810.09980.004-0.00670.15060.00750.0189-4.961-18.0813-61.136
352.7751-2.8565-1.52817.03690.72984.2219-0.13650.1484-0.4513-0.13310.00330.66970.4502-0.30020.13320.1512-0.0067-0.00090.27750.00180.1025-14.6845-17.7571-64.6313
369.5738-6.0651-4.49959.56033.86346.2216-0.0003-0.08890.13310.1473-0.00970.097-0.17010.18950.010.1134-0.0534-0.03440.17760.02270.02220.524-9.6077-67.087
375.22441.59672.07032.36650.84164.6593-0.09210.0943-0.1728-0.29670.0471-0.03540.04770.07390.04490.0945-0.0005-0.00950.03780.03390.0502-28.7885-15.9192-84.7017
386.44881.52942.69072.36170.52254.2229-0.0204-0.41190.1935-0.0903-0.02940.15740.0485-0.09660.04980.0947-0.0106-0.00840.07970.01890.0285-31.0489-14.3708-79.649
394.67064.25591.16248.39721.27181.45060.0345-0.0166-0.1416-0.2087-0.0268-0.24490.03250.2067-0.00770.09780.01170.01630.17690.0350.1208-20.1533-16.4276-77.3764
405.28125.9438-1.489610.8712-0.630.7143-0.28810.0994-1.61080.3936-0.4899-0.27430.3184-0.2870.7780.4448-0.26780.19230.4967-0.10181.1421-36.6765-26.1682-79.9879
414.0927-0.40191.18040.8439-0.20211.72930.1111-0.1632-0.14560.033-0.0321-0.0346-0.097-0.03-0.0790.1025-0.0478-0.00860.18320.01280.0098-13.8659-13.1077-71.8545
425.3797-1.1383-0.71853.6680.13054.9273-0.0173-0.07260.6715-0.02180.1056-0.3093-0.49060.1699-0.08820.2065-0.09030.00710.18010.00920.1736-1.6226-1.5674-77.974
432.5221-1.91520.33835.14710.06364.85190.0530.0086-0.1826-0.0075-0.0546-0.28570.23980.02870.00160.1865-0.02020.03980.2234-0.00360.2277-14.6838-19.0079-88.9205
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A1 - 9
2X-RAY DIFFRACTION2A10 - 23
3X-RAY DIFFRACTION3A24 - 35
4X-RAY DIFFRACTION4A36 - 45
5X-RAY DIFFRACTION5A46 - 60
6X-RAY DIFFRACTION6A61 - 72
7X-RAY DIFFRACTION7A73 - 84
8X-RAY DIFFRACTION8A85 - 89
9X-RAY DIFFRACTION9A90 - 99
10X-RAY DIFFRACTION10A100 - 104
11X-RAY DIFFRACTION11B17 - 27
12X-RAY DIFFRACTION12B28 - 38
13X-RAY DIFFRACTION13B39 - 46
14X-RAY DIFFRACTION14B47 - 66
15X-RAY DIFFRACTION15B67 - 83
16X-RAY DIFFRACTION16B84 - 96
17X-RAY DIFFRACTION17B97 - 112
18X-RAY DIFFRACTION18C61 - 141
19X-RAY DIFFRACTION19C142 - 193
20X-RAY DIFFRACTION20C194 - 209
21X-RAY DIFFRACTION21D1 - 45
22X-RAY DIFFRACTION22D46 - 55
23X-RAY DIFFRACTION23D56 - 65
24X-RAY DIFFRACTION24D66 - 72
25X-RAY DIFFRACTION25D73 - 79
26X-RAY DIFFRACTION26D80 - 84
27X-RAY DIFFRACTION27D85 - 89
28X-RAY DIFFRACTION28D90 - 94
29X-RAY DIFFRACTION29D95 - 104
30X-RAY DIFFRACTION30E17 - 27
31X-RAY DIFFRACTION31E28 - 38
32X-RAY DIFFRACTION32E39 - 45
33X-RAY DIFFRACTION33E46 - 66
34X-RAY DIFFRACTION34E67 - 83
35X-RAY DIFFRACTION35E84 - 96
36X-RAY DIFFRACTION36E97 - 112
37X-RAY DIFFRACTION37F61 - 97
38X-RAY DIFFRACTION38F98 - 121
39X-RAY DIFFRACTION39F122 - 135
40X-RAY DIFFRACTION40F136 - 146
41X-RAY DIFFRACTION41F147 - 169
42X-RAY DIFFRACTION42F170 - 193
43X-RAY DIFFRACTION43F194 - 206

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