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- PDB-9bdm: Cryo-EM structure of cardiac amyloid fibril from a variant ATTRV1... -

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Basic information

Entry
Database: PDB / ID: 9bdm
TitleCryo-EM structure of cardiac amyloid fibril from a variant ATTRV122delta, single filament morphology
ComponentsTransthyretin
KeywordsPROTEIN FIBRIL / ATTR / Systemic amyloidosis / V122delta / amyloid
Function / homology
Function and homology information


Defective visual phototransduction due to STRA6 loss of function / negative regulation of glomerular filtration / The canonical retinoid cycle in rods (twilight vision) / hormone binding / purine nucleobase metabolic process / Non-integrin membrane-ECM interactions / molecular sequestering activity / phototransduction, visible light / retinoid metabolic process / Retinoid metabolism and transport ...Defective visual phototransduction due to STRA6 loss of function / negative regulation of glomerular filtration / The canonical retinoid cycle in rods (twilight vision) / hormone binding / purine nucleobase metabolic process / Non-integrin membrane-ECM interactions / molecular sequestering activity / phototransduction, visible light / retinoid metabolic process / Retinoid metabolism and transport / hormone activity / azurophil granule lumen / Amyloid fiber formation / Neutrophil degranulation / protein-containing complex binding / protein-containing complex / extracellular space / extracellular exosome / extracellular region / identical protein binding
Similarity search - Function
Transthyretin, conserved site / Transthyretin signature 2. / Transthyretin, thyroxine binding site / Transthyretin signature 1. / Transthyretin / Transthyretin/hydroxyisourate hydrolase / Transthyretin/hydroxyisourate hydrolase domain / Transthyretin/hydroxyisourate hydrolase domain superfamily / HIUase/Transthyretin family
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.63 Å
AuthorsNguyen, B.A. / Ahmed, Y. / Saelices, L.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)DP2-HL163810 United States
American Heart Association847236 United States
Welch FoundationI-2121-20220331 United States
CitationJournal: To Be Published
Title: Cryo-EM structure of cardiac amyloid fibril from a variant ATTRV122delta, single filament morphology
Authors: Nguyen, B.A. / Ahmed, Y. / Saelices, L.
History
DepositionApr 12, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 15, 2025Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: Additional map / Part number: 1 / Data content type: Additional map / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: Mask / Part number: 1 / Data content type: Mask / Provider: repository / Type: Initial release
Revision 1.0Oct 15, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Transthyretin
B: Transthyretin
C: Transthyretin
D: Transthyretin
E: Transthyretin


Theoretical massNumber of molelcules
Total (without water)68,3915
Polymers68,3915
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein
Transthyretin / ATTR / Prealbumin / TBPA


Mass: 13678.229 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Organ: Heart
Variant: Fibrillation of transthyretin variant, deletion of V122
Tissue: Cardiac / References: UniProt: P02766
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: fibril of ATTRV122delta / Type: COMPLEX / Details: fibril extracted from cardiac tissue, postmortem. / Entity ID: all / Source: NATURAL
Source (natural)Organism: Homo sapiens (human) / Organ: Heart / Tissue: Cardiac
Buffer solutionpH: 7 / Details: ice-chilled water
Buffer componentConc.: 1 / Name: water / Formula: H2O
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: fibrils were extracted from cardiac tissue using a water-based extraction method.
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 298 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 900 nm
Image recordingAverage exposure time: 3.67 sec. / Electron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 5759

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Processing

EM software
IDNameVersionCategory
1RELION4particle selection
2EPU3.5image acquisition
4CTFFIND4.1CTF correction
7Coot0.9.8.1model fitting
9RELION4.1initial Euler assignment
10RELION4.1final Euler assignment
11RELION4classification
12RELION4.13D reconstruction
13PHENIX1.20.1model refinement
CTF correctionType: NONE
Helical symmertyAngular rotation/subunit: -1.27 ° / Axial rise/subunit: 4.9 Å / Axial symmetry: C1
Particle selectionNum. of particles selected: 498426
3D reconstructionResolution: 3.63 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 32315 / Num. of class averages: 1 / Symmetry type: HELICAL
Atomic model buildingB value: 117 / Protocol: RIGID BODY FIT / Space: REAL
Atomic model buildingPDB-ID: 8TDN

8tdn


Pdb chain-ID: A / Accession code: 8TDN / Source name: PDB / Type: experimental model
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0033640
ELECTRON MICROSCOPYf_angle_d0.4914950
ELECTRON MICROSCOPYf_dihedral_angle_d4.562490
ELECTRON MICROSCOPYf_chiral_restr0.048580
ELECTRON MICROSCOPYf_plane_restr0.006610

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